Modifiers of mesenchymal stem cell quantity and quality

Russell, Athena; Lefavor, Rebecca; Durand, Nisha; Glover, Loren E.; Zubair, Abba C.

doi:10.1111/trf.14597

Cited by 50 publications

(44 citation statements)

References 30 publications

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“…Donor-dependent precondition of the nasal tissue has to be considered to explain these differences in demands on the differentiation medium. Primary cell differentiation, especially using respiratory epithelium, often suffers from variable outcomes with high donor-to-donor variability 34,35 . Donor-dependent variation was also observed in ELISA evaluation resulting in rather high standard deviations.…”

Section: Discussionmentioning

confidence: 99%

Choosing the Right Differentiation Medium to Develop Mucociliary Phenotype of Primary Nasal Epithelial Cells In Vitro

Luengen

Kniebs

Buhl

et al. 2020

Sci Rep

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In vitro differentiation of airway epithelium is of interest for respiratory tissue engineering and studying airway diseases. Both applications benefit from the use of primary cells to maintain a mucociliated phenotype and thus physiological functionality. Complex differentiation procedures often lack standardization and reproducibility. To alleviate these shortfalls, we compared differentiation behavior of human nasal epithelial cells in four differentiation media. Cells were differentiated at the air-liquid interface (ALI) on collagen-coated inserts. Mucociliary differentiation status after five weeks was analyzed by electron microscopy, histology and immunohistochemistry. The amount of ciliation was estimated and growth factor concentrations were evaluated using ELISA. We found that retinoic-acidsupplemented mixture of DMEM and Airway Epithelial Cell Growth Medium gave most promising results to obtain ciliated and mucus producing nasal epithelium in vitro. We discovered the balance between retinoic acid (RA), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF) and fibroblast growth factor β (FGF-β) to be relevant for differentiation. We could show that low VEGF, EGF and FGF-β concentrations in medium correspond to absent ciliation in specific donors. Therefore, our results may in future facilitate donor selection and non-invasive monitoring of ALI cultures and by this contribute to improved standardization of epithelial in vitro culture.

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Section: Discussionmentioning

confidence: 99%

Choosing the Right Differentiation Medium to Develop Mucociliary Phenotype of Primary Nasal Epithelial Cells In Vitro

Luengen

Kniebs

Buhl

et al. 2020

Sci Rep

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“…Furthermore, it should be considered that the possibility of donor variability for MSCs remains. 36 Human capillaries have an average diameter between 5-10 µm depending on the tissue they are located in. In both of our tested hydrogels, mean diameters of the formed structures are in the same range for all supporting cell types despite BM MSCs For these, the diameter was much smaller and the structures have to be considered nonfunctional.…”

Section: Discussionmentioning

confidence: 99%

Influence of Different Cell Types and Sources on Pre-Vascularisation in Fibrin and Agarose–Collagen Gels

et al. 2019

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Vascularisation is essential for the development of tailored, tissue-engineered organs and tissues due to diffusion limits of nutrients and the lack of the necessary connection to the cardiovascular system. To pre-vascularize, endothelial cells and supporting cells can be embedded in the scaffold to foster an adequate nutrient and oxygen supply after transplantation. This technique is applied for tissue engineering of various tissues, but there have been few studies on the use of different cell types or cells sources. We compare the effect of supporting cells from different sources on vascularisation. Fibrin gels and agarose-collagen hydrogels were used as scaffolds. The supporting cells were primary human dermal fibroblasts (HDFs), human nasal fibroblasts (HNFs), human mesenchymal stem cells from umbilical cord's Wharton's jelly (WJ MSCs), adipose-derived MSCs (AD MSCs) and femoral bone marrow-derived MSCs (BM MSCs). The tissue constructs were incubated for 14 days and analyzed by twophoton laser scanning microscopy. Vascularisation was supported by all cell types, forming branched networks of tubular vascular structures in both hydrogels. In general, fibrin gels present a higher angiogenic promoting environment compared to agarose-collagen hydrogels and fibroblasts show a high angiogenic potential in co-culture with endothelial cells. In agarose-collagen hydrogels, vascular structures supported by AD MSCs were comparable to our HDF control in terms of volume, area and length. BM MSCs formed a homogeneous network of smaller structures in both hydrogels. This study provides data toward understanding the pre-vascularisation properties of different supporting cell types and sources for tissue engineering of different organs and tissues.

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“…First, isolation of MSCs from adult sources involves invasive and often painful procedures with possible donor site morbidity (Hass, Kasper, Böhm, & Jacobs, 2011). Second, they have variable proliferation and differentiation capabilities, which are further reduced upon passaging in vitro (Russell, Lefavor, Durand, Glover, & Zubair, 2018). Third, the self-renewal and differentiation potential of MSCs is severely affected by donor age, genetics, and exposure to environmental stress (D'Ippolito, Schiller, Ricordi, Roos, & Howard, 1999;Malgieri, Kantzari, Patrizi, & Gambardella, 2010;Mattiucci, Maurizi, Leoni, & Poloni, 2018).…”

mentioning

confidence: 99%

Mesenchymal stem cells: Cell therapy and regeneration potential

Brown

McKee

Bakshi

et al. 2019

J Tissue Eng Regen Med

448

310

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Rapid advances in the isolation of multipotent progenitor cells, routinely called mesenchymal stromal/stem cells (MSCs), from various human tissues and organs have provided impetus to the field of cell therapy and regenerative medicine. The most widely studied sources of MSCs include bone marrow, adipose, muscle, peripheral blood, umbilical cord, placenta, fetal tissue, and amniotic fluid. According to the standard definition of MSCs, these clonal cells adhere to plastic, express cluster of differentiation (CD) markers such as CD73, CD90, and CD105 markers, and can differentiate into adipogenic, chondrogenic, and osteogenic lineages in vitro. However, isolated MSCs have been reported to vary in their potency and self‐renewal potential. As a result, the MSCs used for clinical applications often lead to variable or even conflicting results. The lack of uniform characterization methods both in vitro and in vivo also contributes to this confusion. Therefore, the name “MSCs” itself has been increasingly questioned lately. As the use of MSCs is expanding rapidly, there is an increasing need to understand the potential sources and specific potencies of MSCs. This review discusses and compares the characteristics of MSCs and suggests that the variations in their distinctive features are dependent on the source and method of isolation as well as epigenetic changes during maintenance and growth. We also discuss the potential opportunities and challenges of MSC research with the hope to stimulate their use for therapeutic and regenerative medicine.

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Modifiers of mesenchymal stem cell quantity and quality

Abstract: MSC physiology is significantly influenced by donor variability and tissue of origin and this should be considered when designing clinical trials.

Cited by 50 publications

References 30 publications

Choosing the Right Differentiation Medium to Develop Mucociliary Phenotype of Primary Nasal Epithelial Cells In Vitro

Choosing the Right Differentiation Medium to Develop Mucociliary Phenotype of Primary Nasal Epithelial Cells In Vitro

Influence of Different Cell Types and Sources on Pre-Vascularisation in Fibrin and Agarose–Collagen Gels

Mesenchymal stem cells: Cell therapy and regeneration potential

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