Modular instrumentation for capillary electrophoresis with laser induced fluorescence detection using plug-and-play microfluidic, electrophoretic and optic modules

Liénard--Mayor, Théo; Furter, Jasmine S.; Taverna, Myriam; Pham, Hung Viet; Hauser, Peter C.; Duc, Thanh

doi:10.1016/j.aca.2020.08.025

Cited by 21 publications

(3 citation statements)

References 37 publications

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“…LC is an analytical separation technology based on the analyte's interactions with a mobile and a stationary phase. Various separation regimes have been proven successful for glycan analysis with specified stationary phases [39, 150]. Briefly, RPLC separates based on the hydrophobic interaction between glycan/derivatized glycan and alkyl chains bonded on silica particles.…”

Section: Nanoflow Lcmentioning

confidence: 99%

Recent advances in microscale separation techniques for glycome analysis

Liu,

Otsuka,

Kawai

2024

J of Separation Science

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The glycomic analysis holds significant appeal due to the diverse roles that glycans and glycoconjugates play, acting as modulators and mediators in cellular interactions, cell/organism structure, drugs, energy sources, glyconanomaterials, and more. The glycomic analysis relies on liquid‐phase separation technologies for molecular purification, separation, and identification. As a miniaturized form of liquid‐phase separation technology, microscale separation technologies offer various advantages such as environmental friendliness, high resolution, sensitivity, fast speed, and integration capabilities. For glycan analysis, microscale separation technologies are continuously evolving to address the increasing challenges in their unique manners. This review discusses the fundamentals and applications of microscale separation technologies for glycomic analysis. It covers liquid‐phase separation technologies operating at scales generally less than 100 µm, including capillary electrophoresis, nanoflow liquid chromatography, and microchip electrophoresis. We will provide a brief overview of glycomic analysis and describe new strategies in microscale separation and their applications in glycan analysis from 2014 to 2023.

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Section: Nanoflow Lcmentioning

confidence: 99%

Recent advances in microscale separation techniques for glycome analysis

Liu,

Otsuka,

Kawai

2024

J of Separation Science

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“…However, commercial fluorescence detectors, which can be connected to already present instruments, are also available. For FITC, those instruments typically have an LOD in the nanomolar range [313,[549][550][551]. Research on home-built LIF and LED-induced fluorescence detectors is also an ongoing topic.…”

Section: 1mentioning

confidence: 99%

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

et al. 2023

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This review is in support of the development of selective, precise, fast, and validated capillary electrophoresis (CE) methods. It follows up a similar article from 1998, Wätzig H, Degenhardt M, Kunkel A. “Strategies for capillary electrophoresis: method development and validation for pharmaceutical and biological applications,” pointing out which fundamentals are still valid and at the same time showing the enormous achievements in the last 25 years. The structures of both reviews are widely similar, in order to facilitate their simultaneous use. Focusing on pharmaceutical and biological applications, the successful use of CE is now demonstrated by more than 600 carefully selected references. Many of those are recent reviews; therefore, a significant overview about the field is provided. There are extra sections about sample pretreatment related to CE and microchip CE, and a completely revised section about method development for protein analytes and biomolecules in general. The general strategies for method development are summed up with regard to selectivity, efficiency, precision, analysis time, limit of detection, sample pretreatment requirements, and validation.

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“…Most studies of TAs in the DBS not only require the extraction of arsenic using the expensive and bulky instruments such as centrifuges and microwave digesters but also require the pretreatment for 90 min or more, which may be detrimental to the study of the unstable arsenic species in air. − ,, Currently, a few small mixing and centrifugation units have been designed to simplify the analytical workflows, including egg beaters, paper gyroscopes, , fidget spinners, , centrifugal microfluidic devices, and ultrasonic devices, , opening up a new idea for the pretreatment of complex samples. The Lego-based devices have the advantages of small size, low cost, and flexible configuration and can be designed for different analysis tasks such as microscope and capillary electrophoresis. − Therefore, it is promising to develop a Lego-based pretreatment device for the extraction and separation of arsenic species in the DBS through the rational configuration of Lego components, so as to achieve the fast and efficient analysis of arsenic species in the DBS by IC-ICP-MS.…”

mentioning

confidence: 99%

Facile Lego-Spinner Pretreatment Device for Analysis of Arsenic Species in Dried Blood Spots by Ion Chromatography-Inductively Coupled Plasma-Mass Spectrometry

et al. 2023

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Dried blood spot (DBS) detection has the advantages of small blood collection, convenience, and reliability, which provides a possibility for large-scale evaluation of arsenic exposure in human population. Herein, a facile Lego-spinner pretreatment device is rationally designed for speciation analysis of arsenic in DBSs by ion chromatographyinductively coupled plasma-mass spectrometry (IC-ICP-MS). In the mixing mode of the Lego-spinner, the magnetic stir bar in the centrifuge tube rotates under a magnetic field to assist the dispersive extraction of arsenic species in the DBS with reagents. In the centrifugation mode of the Legospinner, the arsenic extract is separated from the blood matrix for the subsequent IC-ICP-MS analysis. For the DBS prepared from 80 μL of whole blood, the whole pretreatment operation can be completed within 25 min. The detection limits of arsenobetaine, arsenite, dimethylarsenate, monomethylarsonate, and arsenate in the DBS are 0.09−0.15 μg L −1 , and precisions are <11%. The concentrations of these five arsenic species are highly correlated between whole blood and the DBS (r 2 > 0.97), and Bland−Altman analysis indicates that the concentration difference of arsenic species between whole blood and the DBS is within ±20%. The DBS sampling approach can effectively preserve arsenic species for at least 30 days at 4 °C, and the contents of arsenic species in the DBS prepared from capillary blood are in a reasonable agreement with those of venous whole blood (gold standard). This Lego-spinner provides a handy and efficient tool for fast extraction of arsenic species in DBSs, facilitating the in-depth study of arsenic migration and transformation in the human body.

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Modular instrumentation for capillary electrophoresis with laser induced fluorescence detection using plug-and-play microfluidic, electrophoretic and optic modules

Cited by 21 publications

References 37 publications

Recent advances in microscale separation techniques for glycome analysis

Recent advances in microscale separation techniques for glycome analysis

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

Facile Lego-Spinner Pretreatment Device for Analysis of Arsenic Species in Dried Blood Spots by Ion Chromatography-Inductively Coupled Plasma-Mass Spectrometry

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