Modular Redesign of a Cationic Lytic Peptide To Promote the Endosomal Escape of Biomacromolecules

Azuma, Yusuke; Imai, Hidenori; Kawaguchi, Yusuke; Nakase, Ikuhiko; Kimura, Hiroshi; Futaki, Shiroh

doi:10.1002/ange.201807534

Cited by 6 publications

(1 citation statement)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This determined selective membrane lytic activity toward endosomal membranes and not cellular ones. 102 All the aforementioned endocytic processes can easily determine the internalization of larger cargos, such as proteins or siRNAs. The appealing possibility of vehiculating big biomolecules inside the cell opened up a vast research field on synthetic peptides that could either undergo endocytosis themselves, promote the endocytic process for other peptides, 103 or induce endosomal escape.…”

Section: Endosomal Escapementioning

confidence: 99%

Artificial peptides to induce membrane denaturation and disruption and modulate membrane composition and fusion

Lāce

Cotroneo

Hesselbarth

et al. 2022

Journal of Peptide Science

View full text Add to dashboard Cite

Membranes consisting of phospholipid bilayers are an essential constituent of eukaryotic cells and their compartments. The alteration of their composition, structure, and morphology plays an important role in modulating physiological processes, such as transport of molecules, cell migration, or signaling, but it can also lead to lethal effects. The three main classes of membrane‐active peptides that are responsible for inducing such alterations are cell‐penetrating peptides (CPPs), antimicrobial peptides (AMPs), and fusion peptides (FPs). These peptides are able to interact with lipid bilayers in highly specific and tightly regulated manners. They can either penetrate the membrane, inducing nondestructive, transient alterations, or disrupt, permeabilize, or translocate through it, or induce membrane fusion by generating attractive forces between two bilayers. Because of these properties, membrane‐active peptides have attracted the attention of the pharmaceutical industry, and naturally occurring bioactive structures have been used as a platform for synthetic modification and the development of artificial analogs with optimized therapeutic properties to transport biologically active cargos or serve as novel antimicrobial agents. In this review, we focus on synthetic membrane interacting peptides with bioactivity comparable with their natural counterparts and describe their mechanism of action.

show abstract

Section: Endosomal Escapementioning

confidence: 99%

Artificial peptides to induce membrane denaturation and disruption and modulate membrane composition and fusion

Lāce

Cotroneo

Hesselbarth

et al. 2022

Journal of Peptide Science

View full text Add to dashboard Cite

show abstract

A Multifunctional Peptide‐Conjugated AIEgen for Efficient and Sequential Targeted Gene Delivery into the Nucleus

et al. 2019

View full text Add to dashboard Cite

Gene therapy has immense potential as a therapeutic approach to serious diseases. However, efficient delivery and real‐time tracking of gene therapeutic agents have not been solved well for successful gene‐based therapeutics. Herein we present a versatile gene‐delivery strategy for efficient and visualized delivery of therapeutic genes into the targeted nucleus. We developed an integrin‐targeted, cell‐permeable, and nucleocytoplasmic trafficking peptide‐conjugated AIEgen named TDNCP for the efficient and sequential targeted delivery of an antisense single‐stranded DNA oligonucleotide (ASO) and tracking of the delivery process into the nucleus. As compared with TDNCP/siRNA‐NPs (siRNA functions mainly in the cytoplasm), TDNCP/ASO‐NPs (ASO functions mainly in the nucleus) exhibited a better interference effect, which further indicates that TDNCP is a nucleus‐targeting vector. Moreover, TDNCP/ASO‐NPs showed a favorable tumor‐suppressive effect in vivo.

show abstract

Cell‐Penetrating Dynamic‐Covalent Benzopolysulfane Networks

et al. 2019

View full text Add to dashboard Cite

Cyclic oligochalcogenides (COCs) are emerging as promising systems to penetrate cells.C learly better than and different to the reported diselenolanes and epidithiodiketopiperazines,w ei ntroduce the benzopolysulfanes (BPS), which show efficient delivery,i nsensitivity to inhibitors of endocytosis,and compatibility with substrates as large as proteins.This high activity coincides with high reactivity,s electively toward thiols,e xceeding exchange rates of disulfides under tension. The result is ad ynamic-covalent network of extreme sulfur species,i ncluding cyclic oligomers,from dimers to heptamers, with up to nineteen sulfurs in the ring. Selection from this unfolding adaptive network then yields the reactivities and selectivities needed to access new uptake pathways.Contrary to other COCs,B PS show high retention on thiol affinity columns.T he identification of new modes of cell penetration is important because they promise new solutions to challenges in delivery and beyond.

show abstract

Modular Redesign of a Cationic Lytic Peptide To Promote the Endosomal Escape of Biomacromolecules

Cited by 6 publications

References 47 publications

Artificial peptides to induce membrane denaturation and disruption and modulate membrane composition and fusion

Artificial peptides to induce membrane denaturation and disruption and modulate membrane composition and fusion

A Multifunctional Peptide‐Conjugated AIEgen for Efficient and Sequential Targeted Gene Delivery into the Nucleus

Cell‐Penetrating Dynamic‐Covalent Benzopolysulfane Networks

Contact Info

Product

Resources

About