Modularly assembled multiplex prime editors for simultaneous editing of agronomically important genes in rice

Gupta, Ajay; Liu, Bo; Raza, Saad; Chen, Qi-Jun; Yang, Bing

doi:10.1016/j.xplc.2023.100741

Cited by 9 publications

(2 citation statements)

References 36 publications

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“…Most of these mutations showed reliable tolerance to aryloxyphenoxypropionate herbicides, and some mutations, such as the I1879S, P1927Y, and W2097G mutations, were identified for the first time in plants. Gupta et al (2023) developed a modular assembly‐based multiple PE system in rice, in which four genes can be simultaneously edited in a single transformation experiment. This study employed the editing system to a construct that included four genes, two herbicide‐tolerance genes ( OsEPSPS and OsALS ) and two Xoo resistance genes ( TFIIAγ5 and OsSWEET11a ).…”

Section: Technologies To Induce Herbicide Resistance In Ricementioning

confidence: 99%

“…Sixteen different types of amino acid substitution mutations were found to be closely related to herbicide resistance. Most of these mutations showed reliable tolerance to aryloxyphenoxypropionate herbicides, and some mutations, such as the I1879S, P1927Y, and W2097G mutations, were identified for the first time in plants Gupta et al (2023). developed a modular assembly-based multiple PE system in rice, in which four genes can be simultaneously edited in a single transformation experiment.…”

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A Critical Review of Conventional and Modern Approaches to Develop Herbicide‐Resistance in Rice

Sang,

Wang,

Yao

et al. 2024

Physiologia Plantarum

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Together with rice, weeds strive for nutrients and space in farmland, resulting in reduced rice yield and quality. Planting herbicide‐resistant rice varieties is one of the effective ways to control weeds. In recent years, a series of breakthroughs have been made to generate herbicide‐resistant germplasm, especially the emergence of biotechnological tools such as gene editing, which provides an inherent advantage for the knock‐out or knock‐in of the desired genes. In order to develop herbicide‐resistant rice germplasm resources, gene manipulation has been conducted to enhance the herbicide tolerance of rice varieties through the utilization of techniques such as physical and chemical mutagenesis, as well as genome editing. Based on the current research and persisting problems in rice paddy fields, research on the generation of herbicide‐resistant rice still needs to explore genetic mechanisms, stacking multiple resistant genes in a single genotype, and transgene‐free genome editing using the CRISPR system. Current rapidly developing gene editing technologies can be used to mutate herbicide target genes, enabling targeted genes to maintain their biological functions, and reducing the binding ability of target gene encoded proteins to corresponding herbicides, ultimately resulting in herbicide‐resistant crops. In this review article, we have summarized the utilization of conventional and modern approaches to develop herbicide‐resistant cultivars in rice as an effective strategy for weed control in paddy fields, and discussed the technology and research directions for creating herbicide‐resistant rice in the future.

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Section: Technologies To Induce Herbicide Resistance In Ricementioning

confidence: 99%

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confidence: 99%