Modulating Effects of Pycnogenol® on Oxidative Stress and DNA Damage Induced by Sepsis in Rats

Taner, Gökçe; Aydın, Sevtap; Bacanlı, Merve; Sarıgöl, Zehra; Sahin, Tevfik Tolga; Başaran, Ahmet; Başaran, Nurşen

doi:10.1002/ptr.5184

Cited by 48 publications

(38 citation statements)

References 56 publications

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“…The severity of tissue injury induced by sepsis is correlated with TNF-alpha level and there is a relation between inflammation and oxidative stress (26). In our study, as in our previous data (13)(14)(15), plasma TNF-alpha levels significantly increased in the septic rats, indicating an inflammatory response to sepsis. Single and double DNA strand breaks, base modifications, fragmentation of deoxyribose, formation of DNA-protein cross-links as well as abasic sites may occur as a result of oxidative DNA damage.…”

Section: Discussionsupporting

confidence: 86%

“…The decreases in GSH and increases in MDA levels were determined in the hepatic and renal tissues of septic rats (25). In our study, as for our previous data (13)(14)(15), the increases in MDA levels and decreases in GSH levels and SOD and GPX activities in the liver and kidney were shown in the septic rats compared to the sham group.…”

Section: Discussionsupporting

confidence: 84%

“…At the studied dose, 100% of the animals remained alive. In our previous studies with the phenolic compounds pycnogenol®, ferulic acid, and rosmarinic acid, the dosages of 100 mg/kg bw ip were also used in the experimental sepsis model (13)(14)(15). In these studies evaluated independently form the same local animal ethic approval to reduce animal numbers according to the principles of 3Rs, sham and sepsis groups were common; thus the data from sham and sepsis groups were the same in these studies carried out at the same time (13)(14)(15)(16).…”

Section: Experimental Designmentioning

confidence: 99%

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Resveratrol Protects Sepsis-Induced Oxidative DNA Damage in Liver and Kidney of Rats

Aydın¹,

Şahin²,

Bacanlı³

et al. 2016

Balkan Med J

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Background:The increases of free radicals have been proposed to be involved in the pathogenesis of sepsis, which leads to multiple-organ dysfunction syndromes. The uses of antioxidants as a complementary tool in the medical care of oxidative stress-related diseases have attracted attention of researchers. Resveratrol (RV) has suggested being antioxidant, anti-proliferative, and anti-inflammatory effects in various experimental models and clinical settings. Aims: This study was undertaken to evaluate the protective effects of RV on oxidative DNA damage induced by sepsis in the liver and kidney tissues of Wistar albino rats. Study Design: Animal experimentation. Methods: Four experimental groups consisting of eight animals for each was created using a total of thirty-two male Wistar albino rats. Sham group was given 0.5 mL of saline intra-peritoneal (ip) only following laparatomy. Sepsis group was given 0.5 mL saline ip only following the induction of sepsis. RV-treated group was given a dose of 100 mg/kg ip RV in 0.5 mL saline following laparatomy. RVtreated sepsis group was given 100 mg/kg ip RV in 0.5 mL saline following the induction of sepsis. A model of sepsis was created by cecal ligation and puncture technique. In the liver and kidney tissues, oxidative stress parameters (malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPX)) and a proinflammatory cytokine (tumor necrosis factor alpha (TNF-alpha)), were evaluated spectrophotometrically and DNA damage was determined by the alkaline single cell gel electrophoresis (comet assay) technique using formamidopyrimidine DNA glycosylase protein. Results:In the RV-treated sepsis group, the levels of MDA and TNF-alpha were lower and GSH levels, SOD and GPX activities were higher than in the septic rats (p<0.05). RV treatment significantly reduced the sepsis-induced oxidative DNA damage in the liver and kidney cells (p<0.05). Conclusion: It is suggested that RV treatment might reduce the sepsis-induced oxidative DNA damages in sepsis-related diseases; however, there is a need for more studies to clear up the protective mechanisms of RV against sepsis.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 84%

Section: Experimental Designmentioning

confidence: 99%

See 1 more Smart Citation

Resveratrol Protects Sepsis-Induced Oxidative DNA Damage in Liver and Kidney of Rats

Aydın¹,

Şahin²,

Bacanlı³

et al. 2016

Balkan Med J

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“…In a study conducted on the efficacy of pycnogenol in wound healing in diabetic rats, it was reported that Pycnogenol is a feasible option to accelerate wound healing 23 . In a study using an experimental sepsis model in rats, it was reported that the production of free oxygen radicals in rats treated with Pycnogenol was significantly reduced 24 . In a study conducted in rats, it was reported that Pycnogenol facilitated the healing of colon anastomoses 25 .…”

Section: ■ Discussionmentioning

confidence: 99%

The effect of pycnogenol on lymphatic nodes and adhesion during in a peritoneal adhesion model in rats

Göret¹,

Göret

Kiraz

et al. 2018

Acta Cir. Bras.

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Purpose: To investigate the effects of pycnogenol on peritoneal adhesions and additionally to investigate the immunohistochemical effects of free oxygen radicals and reactive lymph nodes detected in the adhesive tissue that was sampled surrounding the cecum on intraabdominal adhesions. Methods: Twenty-seven Wistar Albino rats were divided into three groups. In group 1 (sham), laparotomy was performed and stitched up. In group 2 (control), after laparotomy was performed, punctate hemorrhage was induced by cecal abrasion in the cecum and each rat was intraperitoneally administered 2 cc of saline. In group 3 (experimental), after laparotomy was performed, punctate hemorrhage was induced by cecal abrasion in the cecum and each rat was intraperitoneally administered a sterile Pycnogenol derivative. The rats in all groups were re-laparotomized on postoperative day 7; samples were obtained from the peritoneal tissue surrounding the cecum, and the rats were sacrificed. Results: In group 3, there was a statistically significant difference in terms of inflammation, lymph node size, and free oxygen radicals; these parameters tended to increase. In terms of fibrosis evaluated using H&E and MT, there was no significant difference between groups 2 and 3. Conclusions: No positive outcomes indicating that pycnogenol reduces intra-abdominal adhesions were obtained. However, it caused severe inflammation in the tissue. Moreover, a significant increase in lymph node size was detected secondary to inflammation. Additionally, in immunohistochemical analyses conducted to detect oxidative stress, pycnogenol increased the production of free oxygen radicals in the tissue.

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“…The dose of PYC ® was selected according to our previous study. 18 After the end of this procedure, blood samples obtained through cardiac puncture were collected into preservative-free heparin tubes for the biochemical and the DNA damage evaluations.…”

Section: Experimental Protocolmentioning

confidence: 99%

The Ameliorative Effects of Pycnogenol<sup>®</sup> on Liver Ischemia-Reperfusion Injury in Rats

Tokaç¹,

Bacanlı²,

Dumlu³

et al. 2017

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Modulating Effects of Pycnogenol® on Oxidative Stress and DNA Damage Induced by Sepsis in Rats

Cited by 48 publications

References 56 publications

Resveratrol Protects Sepsis-Induced Oxidative DNA Damage in Liver and Kidney of Rats

Resveratrol Protects Sepsis-Induced Oxidative DNA Damage in Liver and Kidney of Rats

The effect of pycnogenol on lymphatic nodes and adhesion during in a peritoneal adhesion model in rats

The Ameliorative Effects of Pycnogenol<sup>®</sup> on Liver Ischemia-Reperfusion Injury in Rats

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