Modulating oxidative stress counteracts specific antigen‐induced regulatory T‐cell apoptosis in mice

Abstract

Treg are known to have a central role in orchestrating immune responses, but less is known about the destiny of Treg after being activated by specific Ags. This study aimed to investigate the role of superoxide dismutase, an active molecule in the regulation of oxidative stress in the body, in the prevention of Treg apoptosis induced by specific Ags. Ag‐specific Tregs were isolated from the DO11.10 mouse intestine. A food allergy mouse model was developed with ovalbumin as the specific Ag and here, we observed that exposure to specific Ag induced Treg apoptosis through converting the precursor of TGF‐β to its mature form inside the Tregs. Oxidative stress was induced in Tregs upon exposure to specific Ags, in which Smad3 bound the latency‐associated peptide to induce its degradation, converting the TGF‐β precursor to its mature form, TGF‐β. Suppressing oxidative stress in Tregs alleviated the specific Ag‐induced Treg apoptosis in in vitro experiments and suppressed experimental food allergy by preventing the specific Ag‐induced Treg apoptosis in the intestine. In conclusion, exposure to specific Ags induces Treg apoptosis and it can be prevented by upregulating superoxide dismutase or suppressing reactive oxidative species in Tregs.

Psychological stress to ovalbumin peptide‐specific T‐cell receptor transgenic mice impairs the suppressive ability of type 1 regulatory T cell

“…The general characteristic of Tr1 cells is its secretion of interleukin-10 (IL-10) and the absence of the expression Foxp3 [1,2]. Tr1 cells have attracted greater attention because of their role in peripheral immune tolerance [3,4]. Published data indicate that the regulatory T cell dysfunction is involved in ovalbumin (OVA)-induced allergy responses, psychological stress, oxidative stress, and immunological peripheral tolerance [3][4][5].…”

“…Tr1 cells have attracted greater attention because of their role in peripheral immune tolerance [3,4]. Published data indicate that the regulatory T cell dysfunction is involved in ovalbumin (OVA)-induced allergy responses, psychological stress, oxidative stress, and immunological peripheral tolerance [3][4][5]. Tr1 cells are considered a potential tool for the treatment of autoimmune diseases, cancer and organ transplant rejection [5].…”

Ozone Pollution, Oxidative Stress, Regulatory T Cells and Antioxidants

“…For example, hydrogen peroxide (H 2 O 2 ) secreted by tumor-associated macrophages promotes tumorigenesis by reducing the activity of cytotoxic T cells and NK cells [ 49 ]. This suggests that ROS modulate the behavior of cancer cells and T cells, so that according to their increase or decrease in the tumor microenvironment, they can promote or slow down tumor progression [ 56 ].…”

From waste to energy storage: post-consumer waste expanded polystyrene/rGO composite as a high performance self-standing electrode for coin cell supercapacitors

“…14 Similar to this, polystyrene graed on rGO via an emulsion polymerization method showed improved dielectric behavior of the composite. 15 An analytical device fabricated with a polystyrene@rGO composite prepared via an electrostatic interaction process showed promising performance for the application of biosensors. 16 The mechanical mixing of the EPS with the rGO at high rpm can prevent the agglomeration of the rGO over EPS which is advantageous for the continuous interconnected network.…”