Modulating the biological function of protein by tailoring the adsorption orientation on nanoparticles

Jain, Akhil; Trindade, Gustavo F.; Hicks, Jacqueline M.; Potts, Jordan; Rahman, Ruman; Hague, Richard; Amabilino, David B.; Pérez-García, Lluïsa; Rawson, Frankie J.

doi:10.1016/j.jcis.2020.12.025

Cited by 20 publications

(19 citation statements)

References 80 publications

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“…Another approach is the advanced application of TRPBCs for temperature-controlled protein orientation. The orientation of proteins on the surface plays a crucial role in the binding of cell receptors and ultimately defines their targeting efficiency [ 26 ]. TRPBCs for temperature-controlled protein orientation, first, should not cause denaturation of the adsorbed proteins, and second, the temperature of the transition should be close to physiological (appropriate temperature).…”

Section: Introductionmentioning

confidence: 99%

Temperature-Responsive Polymer Brush Coatings for Advanced Biomedical Applications

et al. 2022

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Modern biomedical technologies predict the application of materials and devices that not only can comply effectively with specific requirements, but also enable remote control of their functions. One of the most prospective materials for these advanced biomedical applications are materials based on temperature-responsive polymer brush coatings (TRPBCs). In this review, methods for the fabrication and characterization of TRPBCs are summarized, and possibilities for their application, as well as the advantages and disadvantages of the TRPBCs, are presented in detail. Special attention is paid to the mechanisms of thermo-responsibility of the TRPBCs. Applications of TRPBCs for temperature-switchable bacteria killing, temperature-controlled protein adsorption, cell culture, and temperature-controlled adhesion/detachment of cells and tissues are considered. The specific criteria required for the desired biomedical applications of TRPBCs are presented and discussed.

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Section: Introductionmentioning

confidence: 99%

Temperature-Responsive Polymer Brush Coatings for Advanced Biomedical Applications

et al. 2022

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“…The UV-VIS spectrum of d -/ l -Au NPs compared with Au NPs in the absorption maximum shifts from 520 to 522 nm ( Figure 1 A). Circular dichroism (CD) was used to determine the stereo configuration of chiral Au NPs [ 47 ]. As shown in Figure 1 B, l -Au NPs and d -Au NPs had an opposite characteristic peak in the wavelength range of 20–250 nm, which were consistent with L-cysteine and d -cysteine, respectively.…”

Section: Resultsmentioning

confidence: 99%

Plasmon-Enhanced Antibacterial Activity of Chiral Gold Nanoparticles and In Vivo Therapeutic Effect

Wang

Zhang

et al. 2021

Nanomaterials

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d-cysteine (d-cys) has been demonstrated to possess an extraordinary antibacterial activity because of its unique steric configuration. However, inefficient antibacterial properties seriously hinder its wide applications. Here, cysteine-functionalized gold nanoparticles (d-/l-Au NPs) were prepared by loading d-/l-cysteine on the surface of gold nanoparticles for the effective inhibition of Escherichia coli (E. coli) in vitro and in vivo, and the effects on the intestinal microflora in mice were explored during the treatment of E. coli infection in the gut. We found that the antibacterial activity of d-/l-Au NPs was more than 2–3 times higher than pure d-cysteine, l-cysteine and Au NPs. Compared with l-Au NPs, d-Au NPs showed the stronger antibacterial activity, which was related to its unique steric configuration. Chiral Au NPs showed stronger destructive effects on cell membrane compared to other groups, which further leads to the leakage of the cytoplasm and bacterial cell death. The in vivo antibacterial experiment illustrated that d-Au NPs displayed impressive antibacterial activity in the treatment of E. coli-infected mice comparable to kanamycin, whereas they could not affect the balance of intestinal microflora. This work is of great significance in the development of an effective chiral antibacterial agent.

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“…Anionic ligands on AuNPs disrupt the tertiary structure of cyt c, while cationic and neutral ligands maintain cyt c structure. 153 Furthermore, the surface charge of AuNPs determines the apoptotic and peroxidase activity of cyt c, which is governed by the accessibility of its heme ring in relation to its structure and orientation on AuNPs. Secondary structural changes of HSA adsorbed onto AuNPs depend on the curvature of the AuNPs, the type of surface ligands, and the pH of the medium.…”

Section: Role Of Nanoparticles In Protein Corona Conformationmentioning

confidence: 99%

Nanoparticle protein corona: from structure and function to therapeutic targeting

et al. 2023

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Modulating the biological function of protein by tailoring the adsorption orientation on nanoparticles

Cited by 20 publications

References 80 publications

Temperature-Responsive Polymer Brush Coatings for Advanced Biomedical Applications

Temperature-Responsive Polymer Brush Coatings for Advanced Biomedical Applications

Plasmon-Enhanced Antibacterial Activity of Chiral Gold Nanoparticles and In Vivo Therapeutic Effect

Nanoparticle protein corona: from structure and function to therapeutic targeting

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