Modulating the catalytic activity of enzyme-like nanoparticles through their surface functionalization

Cao‐Milán, Roberto; He, Luke D.; Shorkey, Spencer A.; Tonga, Gülen Yesilbag; Wang, Lisheng; Zhang, Xianzhi; Uddin, Imad; Das, Rahul; Sulak, Mine; Rotello, Vincent M.

doi:10.1039/c7me00055c

Cited by 38 publications

(31 citation statements)

References 24 publications

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“…These results confirm that by combining immobilized laccase with the nanocomposites, the percentage of dye decolorization increased with respect to free laccase and just immobilized laccase in the filter. This synergistic behavior has been previously described for systems where immobilized lipase enzyme from Candida rugosa was applied for the lipolytic activity in conjunction with gold nanoparticles [ 57 , 58 ]. In this study, the improved behavior was attributed to the hydrophobic/hydrophilic interaction of the nanoparticles [ 57 ].…”

Section: Discussionmentioning

confidence: 69%

Congo Red Decolorization Using Textile Filters and Laccase-Based Nanocomposites in Continuous Flow Bioreactors

et al. 2020

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Removal of azo and diazo dye content from textile industry wastewaters is crucial due to their environmental impact. Here, we report on the use of the fungal laccase from Pycnoporus sanguineus CS43 immobilized on silica nanoparticles and entrapped in textile-based filters for the degradation of Congo Red. Laccase immobilization and synthesis of the nanocomposites were carried out by two different methods, one in the presence of acetone and the second using water as solvent. This led to a change in the hydrophobicity of the obtained biofilters. Successful preparation of the nanocomposites was confirmed via FTIR spectroscopy. Changes in the secondary structure of the enzyme were inspected through the second derivative of the FTIR spectra. Six different types of filter were fabricated and tested in a continuous flow bioreactor in terms of their decolorization capabilities of Congo Red. The results indicate removal efficiencies that approached 40% for enzymes immobilized on the more hydrophobic supports. Backscattered electron (BSE) images of the different filters were obtained before and after the decolorization process. Percentage of decolorization and activity loss were determined as a function of time until a plateau in decolorization activity was reached. Experimental data was used to recreate the decolorization process in COMSOL Multiphysics® (Stockholm, Sweden). These simulations were used to determine the proper combination of parameters to maximize decolorization. Our findings suggest that the treatment of textile-based filters with immobilized laccase in conjunction with hydrophobic nanocomposites provides a suitable avenue to achieve more efficient laccase dye decolorization (39%) than that obtained with similar filters treated only with free laccase (8%). Filters treated with silica-based nanocomposites and immobilized laccases showed an increase in their decolorization capability, probably due to changes in their wetting phenomena.

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Section: Discussionmentioning

confidence: 69%

Congo Red Decolorization Using Textile Filters and Laccase-Based Nanocomposites in Continuous Flow Bioreactors

et al. 2020

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“…Development of new nanocatalysts, including NZs, with higher catalytic activity expands their applications in bioanalytics. As a rule, the activity of NZs is lower than that of enzymes and their catalytic performance strongly depends on: (1) the method of synthesis (pH, reaction time and temperature) [25]; the composition of the nanomaterial [16,65]; the shape, size, dispersity and final morphology [66]; the mass ratio of the components in the nanocomposite [67]; and surface functionalization [40,[68][69][70].…”

Section: Catalytic Performance Of Nanozymesmentioning

confidence: 99%

Synthesis, Catalytic Properties and Application in Biosensorics of Nanozymes and Electronanocatalysts: A Review

Stasyuk

Smutok

Demkiv

et al. 2020

Sensors

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The current review is devoted to nanozymes, i.e., nanostructured artificial enzymes which mimic the catalytic properties of natural enzymes. Use of the term “nanozyme” in the literature as indicating an enzyme is not always justified. For example, it is used inappropriately for nanomaterials bound with electrodes that possess catalytic activity only when applying an electric potential. If the enzyme-like activity of such a material is not proven in solution (without applying the potential), such a catalyst should be named an “electronanocatalyst”, not a nanozyme. This paper presents a review of the classification of the nanozymes, their advantages vs. natural enzymes, and potential practical applications. Special attention is paid to nanozyme synthesis methods (hydrothermal and solvothermal, chemical reduction, sol-gel method, co-precipitation, polymerization/polycondensation, electrochemical deposition). The catalytic performance of nanozymes is characterized, a critical point of view on catalytic parameters of nanozymes described in scientific papers is presented and typical mistakes are analyzed. The central part of the review relates to characterization of nanozymes which mimic natural enzymes with analytical importance (“nanoperoxidase”, “nanooxidases”, “nanolaccase”) and their use in the construction of electro-chemical (bio)sensors (“nanosensors”).

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“…The catalytic and enzymatic properties of NP‐based biosensing platforms have also been highlighted due to their rapid sensing process, visualization by the naked eye and high sensitivity . In particular, since MeNPs possess enzyme‐like activities, they can be utilized in the place of an enzyme in an enzyme‐linked immunosorbent assay (ELISA) to catalyze and activate the visualization reagent .…”

Section: Plasmonic and Catalytic Nanoparticle‐based Biosensing Systemsmentioning

confidence: 99%

High‐Performance Biosensing Systems Based on Various Nanomaterials as Signal Transducers

Lee

Adegoke

Park

2018

Biotechnology Journal

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Recently, highly sensitive and selective biosensors have become necessary for improving public health and well-being. To fulfill this need, high-performance biosensing systems based on various nanomaterials, such as nanoparticles, carbon nanomaterials, and hybrid nanomaterials, are developed. Numerous nanomaterials show excellent physical properties, including plasmonic, magnetic, catalytic, mechanical and fluorescence properties and high electrical conductivities, and these unique and beneficial properties have contributed to the fabrication of high-performance biosensors with various applications, including in optical, electrical, and electrochemical detection platforms. In addition, these properties can be transformed to signals for the detection of biomolecules. In this review, various types of nanomaterial-based biosensors are introduced, and they show high sensitivity and selectivity. In addition, the potential applications of these sensors on the biosensing of several types of biomolecules are also discussed. These nanomaterials-based biosensing systems provide a significant improvement on healthcare including rapid monitoring and early detection of infectious disease for public health.

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Modulating the catalytic activity of enzyme-like nanoparticles through their surface functionalization

Cited by 38 publications

References 24 publications

Congo Red Decolorization Using Textile Filters and Laccase-Based Nanocomposites in Continuous Flow Bioreactors

Congo Red Decolorization Using Textile Filters and Laccase-Based Nanocomposites in Continuous Flow Bioreactors

Synthesis, Catalytic Properties and Application in Biosensorics of Nanozymes and Electronanocatalysts: A Review

High‐Performance Biosensing Systems Based on Various Nanomaterials as Signal Transducers

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