Modulation by heme and zinc protoporphyrin of colonic heme oxygenase-1 and experimental inflammatory bowel disease in the rat

Heme oxygenase-1 induction by (S)-enantiomer of YS-51 (YS-51S), a synthetic isoquinoline alkaloid, inhibits nitric oxide production and nuclear factor-κB translocation in ROS 17/2.8 cells activated with inflammatory stimulants

“…An HO-1 inhibitor ZnPPIX, reversed both of the effects of YS-51S, suppression of iNOS expression and anti-DNA degradation, induced by MIX, indicating that up-regulation of HO-1 is strongly associated with the action of YS-51S. Recently we and others reported that ZnPPIX reduces not only activity but also expression of HO-1 in vivo [39,40]. Indeed, YS-51 concentration-and time-dependently increased HO-1 induction in ROS 17/28 cells.…”

Heme Oxygenase-1 Ameliorates Dextran Sulfate Sodium-induced Acute Murine Colitis by Regulating Th17/Treg Cell Balance

“…HO-1 Activity Assay-HO-1 enzyme activity was assessed by measuring bilirubin production as described previously with modifications (36). The colons were homogenized in 10 mmol/ liter HEPES, 32 mmol/liter sucrose, 1 mmol/liter dithiotreitol (DTT), 0.1 mmol/liter EDTA, 10 g/ml soybean trypsin inhibitor, 10 g/ml leupeptin, and 2 g/ml aprotinin and centrifuged at 18,000 ϫ g for 30 min at 4°C.…”

Heme Oxygenase-1 Exerts a Protective Role in Ovalbumin-induced Neutrophilic Airway Inflammation by Inhibiting Th17 Cell-mediated Immune Response

“…10 Mice-Previously, we found that up-regulation of HO-1 could protect against OVA-sensitized/challenged eosinophilic airway inflammation (16). Thus, we investigated the effect of HO-1 on neutrophilic airway inflammation in DO11.10 mice.…”