Modulation of autophagy by melatonin via sirtuins in stroke: From mechanisms to therapies

Kazemnejad, Somaieh; Tavakol, Shima; Ketabforoush, Arsh Haj Mohamad Ebrahim; Khazaei, Ghasem; Bakhtazad, Atefeh; Mousavizadeh, Kazem; Joghataei, Mohammad Taghi

doi:10.1016/j.lfs.2022.120870

Cited by 10 publications

(6 citation statements)

References 144 publications

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“…Melatonin can modulate autophagy by various pathways. For example, melatonin and its metabolites adjust various sirtuin pathways related to mitochondrial function and autophagy in the case of stroke [50] . In addition, melatonin-based therapeutics modulated mitophagy in macrophages to ameliorate atherosclerosis [51] .…”

Section: Discussionmentioning

confidence: 99%

Melatonin protects TEGDMA-induced preodontoblast mitochondrial apoptosis via the JNK/MAPK signaling pathway

Yu,

Hua,

Zhao

et al. 2024

ABBS

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Resin monomer-induced dental pulp injury presents a pathology related to mitochondrial dysfunction. Melatonin has been regarded as a strong mitochondrial protective bioactive compound from the pineal gland. However, it remains unknown whether melatonin can prevent dental pulp from resin monomer-induced injury. The aim of this study is to investigate the effects of melatonin on apoptosis of mouse preodontoblast cells (mDPC6T) induced by triethylene glycol dimethacrylate (TEGDMA), a major component in dental resin, and to determine whether the JNK/MAPK signaling pathway mediates the protective effect of melatonin. A well-established TEGDMA-induced mDPC6T apoptosis model is adopted to investigate the preventive function of melatonin by detecting cell viability, apoptosis rate, expressions of apoptosis-related proteins, mitochondrial ROS (mtROS) production, mitochondrial membrane potential (MMP) and adenosine triphosphate (ATP) level. Inhibitors of MAPKs are used to explore which pathway is involved in TEGDMA-induced apoptosis. Finally, the role of the JNK/MAPK pathway is verified using JNK agonists and antagonists. Our results show that melatonin attenuates TEGDMA-induced mDPC6T apoptosis by reducing mtROS production and rescuing MMP and ATP levels. Furthermore, mitochondrial dysfunction and apoptosis are alleviated only by the JNK/MAPK inhibitor SP600125 but not by other MAPK inhibitors. Additionally, melatonin downregulates the expression of phosphorylated JNK and counteractes the activating effects of anisomycin on the JNK/MAPK pathway, mimicking the effects of SP600125. Our findings demonstrate that melatonin protects mDPC6T cells against TEGDMA-induced apoptosis partly through JNK/MAPK and the maintenance of mitochondrial function, offering a novel therapeutic strategy for the prevention of resin monomer-induced dental pulp injury.

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Section: Discussionmentioning

confidence: 99%

Melatonin protects TEGDMA-induced preodontoblast mitochondrial apoptosis via the JNK/MAPK signaling pathway

Yu,

Hua,

Zhao

et al. 2024

ABBS

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“…25,26 Fibroblasts synthesize various growth factors and cytokines that play a vital role in wound healing, stimulation, dermal milieu augmentation, epidermal differentiation process, dermal regeneration, and matrix deposition. [27][28][29][30][31][32] Isolating the fibroblast cells is a simple procedure that requires a small biopsy at a non-visible site, typically the retro-auricular area. It has virtually zero risk of hypersensitivity reaction.…”

Section: Discussionmentioning

confidence: 99%

“…Fibroblasts synthesize various growth factors and cytokines that play a vital role in wound healing, stimulation, dermal milieu augmentation, epidermal differentiation process, dermal regeneration, and matrix deposition. 27 – 32 …”

Section: Discussionmentioning

confidence: 99%

Efficacy of fibroblast transplantation in the healing of cutaneous leishmaniasis scar: A case report

Faghihi,

Larijani,

Mohebbi

et al. 2023

SAGE Open Medical Case Reports

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Autologous fibroblast transplantation has been proven to be a promising method in wound healing with no side effects. This is the first study aimed to determine the efficacy and safety of autologous fibroblast cell injection to the atrophic scar caused by cutaneous leishmaniasis as an endemic disease in many middle-eastern countries. It causes chronic skin lesions and permanently disfiguring scars. Autologous fibroblasts were obtained from the patient’s ear skin and were injected intradermally twice at 2-month intervals. Outcomes were measured using ultrasonography, VisioFace, and Cutometer. No adverse reaction was observed. The results showed improvements in epidermal thickness and density, melanin level, and skin lightening. Moreover, the skin elasticity in the scar area increased after the second transplantation. No improvement was observed in dermal thickness and density. A longer follow-up with more patients is recommended to investigate the effectiveness of fibroblast transplantation better.

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“…The link between antioxidants and cardiovascular physiology has a rather long history, but only recently has more space been given to the clinical meaning and relevance that this relationship may have. Specifically, among the antioxidants, special attention was given to melatonin, according to its antihypertensive, antioxidant, and vasoprotective effects [40,41]. The main point was the possibility of acting on the morphostructural alterations induced by EH, improving in the short and long term the expectation and quality of life of the patients involved [5,42,43].…”

Section: Discussionmentioning

confidence: 99%

Essential Hypertension and Oxidative Stress: Novel Future Perspectives

Franco

Sciatti

Favero

et al. 2022

IJMS

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Among cardiovascular diseases, hypertension is one of the main risk factors predisposing to fatal complications. Oxidative stress and chronic inflammation have been identified as potentially responsible for the development of endothelial damage and vascular stiffness, two of the primum movens of hypertension and cardiovascular diseases. Based on these data, we conducted an open-label randomized study, first, to evaluate the endothelial damage and vascular stiffness in hypertense patients; second, to test the effect of supplementation with a physiological antioxidant (melatonin 1 mg/day for 1 year) in patients with essential hypertension vs. hypertensive controls. Twenty-three patients of either gender were enrolled and randomized 1:1 in two groups (control and supplemented group). The plasmatic total antioxidant capacity (as a marker of oxidative stress), blood pressure, arterial stiffness, and peripheral endothelial function were evaluated at the beginning of the study and after 1 year in both groups. Our results showed that arterial stiffness improved significantly (p = 0.022) in supplemented patients. The endothelial function increased too, even if not significantly (p = 0.688), after 1 year of melatonin administration. Moreover, the supplemented group showed a significative reduction in TAC levels (p = 0.041) correlated with the improvement of arterial stiffness. These data suggest that melatonin may play an important role in reducing the serum levels of TAC and, consequently, in improving arterial stiffness.

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Modulation of autophagy by melatonin via sirtuins in stroke: From mechanisms to therapies

Cited by 10 publications

References 144 publications

Melatonin protects TEGDMA-induced preodontoblast mitochondrial apoptosis via the JNK/MAPK signaling pathway

Melatonin protects TEGDMA-induced preodontoblast mitochondrial apoptosis via the JNK/MAPK signaling pathway

Efficacy of fibroblast transplantation in the healing of cutaneous leishmaniasis scar: A case report

Essential Hypertension and Oxidative Stress: Novel Future Perspectives

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