Modulation of cell communication and carcinogenesis.

Kanno, Yoshinobu

doi:10.2170/jjphysiol.35.693

Cited by 59 publications

(11 citation statements)

References 56 publications

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“…In 1980, Schenk [21] examined the expression of gap junctions in laryngeal carcinomas and observed a downregulation of the gap junctions in comparison to the normal epithelium. Furthermore, some authors assume a correlation between the loss of GJIC and an increase in the metastatic potential of a cell population [8,22,23].…”

Section: Discussionmentioning

confidence: 99%

Expression of Gap Junction Proteins (Connexin 26, 30, 32, 43) in Normal Mucosa, Hyperkeratosis and Carcinoma of the Human Larynx

Schneider¹,

Teschner²,

Sudermann

et al. 2002

ORL

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Introduction: Gap junction proteins (connexins = Cx) form transmembrane channels and mediate the transfer of small molecules and ions between the cytoplasm of adjacent cells. Most tissues express several Cx isoforms. The precise combination might play an important role in the maintenance of cell differentiation. Human carcinogenesis is accompanied by aberrant expression and function of Cx. While the larynx is a target organ for many tumor promoters, no data on Cx expression in laryngeal mucosa are available. The goal of the study was to observe the expression of different Cx (Cx26, -30, -32 and -43) in the normal mucosa, hyperkeratoses and carcinomas of the human larynx. Method: The immunofluorescence method was performed in normal (n = 7) and dysplastic (n = 6) laryngeal mucosa and in squamous cell carcinoma (n = 7) using affinity-purified polyclonal rabbit antibodies against the 4 Cx isoforms and FITC-conjugated secondary antibodies. Results: The immunofluorescence staining of the normal human vocal fold’s epithelium showed the expression of Cx26 and Cx30 in the parabasal and intermediate layers, whereas Cx43 was localized in the basal, parabasal and lower intermediate layers. Cx epitopes could not be found in the upper layers. The precanceroses showed a similar expression of the Cx compared to normal laryngeal epithelium. Due to the higher degree of staining observed in dysplastic specimens, a hyperexpression of Cx26, -30 and -43 could be assumed. The squamous cell caricnomas were characterized by inhomogeneous staining for Cx26, -30 and -43. Regions of intensive expression alternated with regions of no expression. Cx32 could not be observed by immunofluorescence staining in laryngeal tissue. Conclusion: In immunohistochemical terms, there was no alteration of the expression of Cx isoforms during carcinogenesis in the laryngeal epithelium. These results do not exclude a loss of functional intercellular gap junction communication by posttranslational modifications of Cx isoforms or disturbed Cx integration into the gap junction channel. Further studies should investigate potential defective gap junctional intercellular communication in cancer cells based on molecular studies.

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Section: Discussionmentioning

confidence: 99%

Expression of Gap Junction Proteins (Connexin 26, 30, 32, 43) in Normal Mucosa, Hyperkeratosis and Carcinoma of the Human Larynx

Schneider¹,

Teschner²,

Sudermann

et al. 2002

ORL

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“…A few lipid droplets are present in the cytoplasm but they are In many types of tissue and cultured cells, the interiors of adjacent cells communicate with each other through cell-to-cell channels [26] . The fine structure of the cellto-cell channel has been well studied and defined as a gap junction [26] . Cell communication is proposed to play an important role in cell growth and differentiation [26] .…”

Section: Fine Structure Of Newborn Mfe and Vfmmentioning

confidence: 99%

“…The fine structure of the cellto-cell channel has been well studied and defined as a gap junction [26] . Cell communication is proposed to play an important role in cell growth and differentiation [26] . The VFSCs in the newborn MFe may communicate with each other for their growth and differentiation.…”

Section: Fine Structure Of Newborn Mfe and Vfmmentioning

confidence: 99%

Functional Histology of the Macula Flava in the Human Vocal Fold – Part 2: Its Role in the Growth and Development of the Vocal Fold

Sato

Umeno²,

Nakashima³

2010

Folia Phoniatr Logop

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Objective: This study aims to clarify the role of the maculae flavae (MFe) during growth and development of the human vocal fold mucosa (VFM). Methods: Our current results concerning the MFe in the human newborn, infant, and child VFM are summarized. Results: Newborns already had immature MFe at the same sites as adults. They were composed of dense masses of vocal fold stellate cells (VFSCs), whereas extracellular matrix components were sparse. VFSCs in the newborn MFe had already started synthesizing extracellular matrices (EM). During infancy, the EM synthesized in the MFe appeared in the VFM to initiate the formation of the three-dimensional extracellular matrix structure of the human VFM. During childhood, MFe including VFSCs continued to synthesize EM such as collagenous, reticular, and elastic fibers, and hyaluronic acid (glycosaminoglycan), which are essential for the human VFM as a vibrating tissue. The MFe in newborns, infants and children were related to the growth and development of the human VFM. Conclusion: Human MFe including VFSCs were inferred to be involved in the metabolism of EM, essential for the viscoelasticity of the human VFM, and are considered to be an important structure in the growth and development of the human VFM.

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“…39 -41 The absence or reduced number of gap junctions and gap-junctional intercellular communication have been observed in a large number of human and animal tumor cell lines. 39,42 The aim of the present study was to investigate which pathways may play a role in Ca 2ϩ signaling in normal and NF1 keratinocytes. The cells were stimulated mechanically in the presence of inhibitors of gap-junctional or ATP-mediated communication to determine which pathways are operative in Ca 2ϩ signaling between these cells.…”

mentioning

confidence: 99%

Altered Calcium-Mediated Cell Signaling in Keratinocytes Cultured from Patients with Neurofibromatosis Type 1

Korkiamäki¹,

Ylä‐Outinen²,

Koivunen³

et al. 2002

The American Journal of Pathology

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Capacitative calcium entry and calcium wave propagation were studied in keratinocytes cultured from control persons and patients with type 1 neurofibromatosis. The cells were stimulated mechanically in the presence of inhibitors of gap-junctional or ATPmediated communication to determine which pathways are operative in Ca 2؉ signaling between these cells. Keratinocytes cultured from patients with type 1 neurofibromatosis (NF1) had a tendency to form cultures with markedly altered calcium-related signaling characteristics. Specifically, the resting Ca 2؉ levels, intracellular Ca 2؉ stores, capacitative calcium influx, and gap-junctional signal transduction were defective in NF1 keratinocytes. Western transfer analysis revealed apparently equal connexin 43 protein levels in normal control and in NF1 keratinocytes. Indirect immunofluorescence, however, demonstrated that connexin 43 was relatively evenly distributed in NF1 cells and did not form typical gap-junctional plaques between keratinocytes. Furthermore, the speed of the calcium wave was reduced in NF1 cells compared to normal keratinocytes. The results demonstrate that keratinocytes cultured from patients with NF1 display altered calcium-mediated signaling between cells.

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Modulation of cell communication and carcinogenesis.

Cited by 59 publications

References 56 publications

Expression of Gap Junction Proteins (Connexin 26, 30, 32, 43) in Normal Mucosa, Hyperkeratosis and Carcinoma of the Human Larynx

Expression of Gap Junction Proteins (Connexin 26, 30, 32, 43) in Normal Mucosa, Hyperkeratosis and Carcinoma of the Human Larynx

Functional Histology of the Macula Flava in the Human Vocal Fold – Part 2: Its Role in the Growth and Development of the Vocal Fold

Altered Calcium-Mediated Cell Signaling in Keratinocytes Cultured from Patients with Neurofibromatosis Type 1

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