Modulation of cellular membrane properties as a potential therapeutic strategy to counter lipointoxication in obstructive pulmonary diseases

Kadri, L.; Ferru-Clément, Romain; Bâcle, Amélie; Payet, Laurie-Anne; Cantereau, Anne; Hélye, Reynald; Becq, Frédéric; Jayle, Christophe; Vandebrouck, Clarisse; Ferreira, Thierry

doi:10.1016/j.bbadis.2018.06.021

Cited by 12 publications

(28 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1A). PC species were analyzed in positive ion mode after the addition of 0.1% (v/v) formic acid as already described (10) (Supplementary Fig. 2A).…”

Section: Introductionmentioning

confidence: 99%

A comprehensive study of phospholipid fatty acid rearrangements in metabolic syndrome: correlations with organ dysfunction

Bâcle

Kadri

Khoury

et al. 2020

Disease Models &Amp; Mechanisms

Self Cite

View full text Add to dashboard Cite

The balance within phospholipids (PL) between Saturated Fatty Acids (SFA) and mono-or poly-Unsaturated Fatty Acids (UFA), is known to regulate the biophysical properties of cellular membranes. As a consequence, perturbating this balance alters crucial cellular processes in many cell types, such as vesicular budding and the trafficking/function of membrane-anchored proteins. The worldwide spreading of the Western-diet, which is specifically enriched in saturated fats, has been clearly correlated with the emergence of a complex syndrome, known as the Metabolic Syndrome (MetS), which is defined as a cluster of risk factors for cardiovascular diseases, type 2 diabetes and hepatic steatosis. However, no clear correlations between diet-induced fatty acid redistribution within cellular PL, the severity/chronology of the symptoms associated to MetS and the function of the targeted organs have been established. In an attempt to fill this gap, we analyzed in the present study PL remodeling in rats exposed during 15 weeks to a High Fat/High Fructose diet (HFHF) in several organs, including known MetS targets. We show that fatty acids from the diet can distribute within PL in a very selective way, with PhosphatidylCholine being the preferred sink for this distribution. Moreover, in the HFHF rat model, most organs are protected from this redistribution, at least during the early onset of MetS, at the exception of the liver and skeletal muscles. Interestingly, such a redistribution correlates with clear-cut alterations in the function of these organs.

show abstract

“…1A). PC species were analyzed in positive ion mode after the addition of 0.1% (v/v) formic acid as already described (10) (Supplementary Fig. 2A).…”

Section: Introductionmentioning

confidence: 99%

A comprehensive study of phospholipid fatty acid rearrangements in metabolic syndrome: correlations with organ dysfunction

Bâcle

Kadri

Khoury

et al. 2020

Disease Models &Amp; Mechanisms

Self Cite

View full text Add to dashboard Cite

show abstract

“…Each frozen sample was first submitted to three rounds of grinding using a Precellys Evolution homogenizer (Bertin Technologies, Montigny-le-Bretonneux, France) and resuspended into 1 ml of water before tranfer into glass tubes containing 500 μL of glass beads (diameter 0.3–0.4 mm; Sigma-Aldrich, Saint-Louis, Missouri, USA). Lipids were extracted using chloroform/methanol (2:1, v/v) and shaking with an orbital shaker (IKAH VXR basic VibraxH - Sigma-Aldrich, Saint-Louis, Missouri, USA) at 1500 rpm during at least 1 h, as already described elsewhere ( 10 ). The final organic phase was evaporated and dissolved in 100 μL dichloromethane for purification of Phospholipids (PL) on a silica column (Bond ELUT-SI - Agilent Technologies, Santa Clara, California, USA).…”

Section: Methodsmentioning

confidence: 99%

A comprehensive study of Phospholipid fatty acid rearrangements in the early onset of the metabolic syndrome: correlations to organ dysfunction

Bâcle

Kadri

Khoury

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

1The balance within phospholipids (PL) between Saturated Fatty Acids (SFA) and mono-or poly-2 Unsaturated Fatty Acids (UFA), is known to regulate the biophysical properties of cellular membranes. 3As a consequence, perturbating this balance alters crucial cellular processes in many cell types, such 4 as vesicular budding and the trafficking/function of membrane-anchored proteins. The worldwide 5 spreading of the Western-diet, which is specifically enriched in saturated fats, has been clearly 6 correlated with the emergence of a complex syndrome, known as the Metabolic Syndrome (MetS), 7 which is defined as a cluster of risk factors for cardiovascular diseases, type 2 diabetes and hepatic 8 steatosis. However, no clear correlations between diet-induced fatty acid redistribution within cellular 9 PL, the severity/chronology of the symptoms associated to MetS and the function of the targeted 10 organs, particularly in the early onset of the disease, have been established. In an attempt to fill this 11 gap, we analyzed in the present study PL remodeling in rats exposed during 15 weeks to a High Fat/High 12 Fructose diet (HFHF) in several organs, including known MetS targets. We show that fatty acids from 13 the diet can distribute within PL in a very selective way, with PhosphatidylCholine being the preferred 14 sink for this distribution. Moreover, in the HFHF rat model, most organs are protected from this 15 redistribution, at least during the early onset of MetS, at the exception of the liver and skeletal muscles. 16Interestingly, such a redistribution correlates with clear-cut alterations in the function of these organs. 17 18

show abstract

“…First, in vitro , one should keep in mind the culture conditions can greatly influence the fatty acid signature of the cells. For example, knowing that the main fatty acid desaturases are strictly oxygen-dependent (see above), oxygen scarcity, which is encountered in patient lungs suffering CF [16] , results in an increase in SFA-enriched PLs in bronchial epithelial cells at the expense of MUFA-containing ones [ 17 , 18 ]. Interestingly, most experiments performed on CF bronchial epithelial cells are generally performed under normoxia, therefore leading to higher MUFA/SFA ratios than encountered in vivo .…”

Section: Constant Dysregulation Of Fatty Acid Profile In Cfmentioning

confidence: 99%

Glued in lipids: Lipointoxication in cystic fibrosis

Vandebrouck

Ferreira

2020

EBioMedicine

Self Cite

View full text Add to dashboard Cite

Cystic Fibrosis (CF) is an autosomal recessive disease caused by mutations in the CF transmembrane regulator (CFTR) gene, which encodes a chloride channel located at the apical surface of epithelial cells. Unsaturated Fatty Acid (UFA) deficiency has been a persistent observation in tissues from patients with CF. However, the impacts of such deficiencies on the etiology of the disease have been the object of intense debates. The aim of the present review is first to highlight the general consensus on fatty acid dysregulations that emerges from, sometimes apparently contradictory, studies. In a second step, a unifying mechanism for the potential impacts of these fatty acid dysregulations in CF cells, based on alterations of membrane biophysical properties (known as lipointoxication), is proposed. Finally, the contribution of lipointoxication to the progression of the CF disease and how it could affect the efficacy of current treatments is also discussed.

show abstract

Modulation of cellular membrane properties as a potential therapeutic strategy to counter lipointoxication in obstructive pulmonary diseases

Cited by 12 publications

References 65 publications

A comprehensive study of phospholipid fatty acid rearrangements in metabolic syndrome: correlations with organ dysfunction

A comprehensive study of phospholipid fatty acid rearrangements in metabolic syndrome: correlations with organ dysfunction

A comprehensive study of Phospholipid fatty acid rearrangements in the early onset of the metabolic syndrome: correlations to organ dysfunction

Glued in lipids: Lipointoxication in cystic fibrosis

Contact Info

Product

Resources

About