Modulation of gene expression in eicosapentaenoic acid and docosahexaenoic acid treated human colon adenoma cells

Habermann, Nina; Lund, Elizabeth K.; Pool‐Zobel, Beatrice L.; Glei, Michael

doi:10.1007/s12263-009-0112-y

Cited by 13 publications

(8 citation statements)

References 10 publications

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“…Secondly, quercetin was found to reduce GSTA1 mRNA expression after 2 h of incubation of differentiated Caco-2 cells at concentrations of 25 µM (48), whereas in our study 50 µM of quercetin showed a nonsignificant increase of GSTA1 protein expression in nondifferentiated cells after 24 h incubation period. Lastly, we found a decrease in GSTP1 expression in LT97 cells after incubation with 10 µM EPA, whereas in a recent study analyzing the gene expression of LT97 cells under the influence of 50 µM EPA, a modulation of the GSTP1 gene was not reported (52). However, a time-dependent effect of EPA on this colorectal adenoma cell line was clearly present, with more upregulated genes after 24 h, as compared to 10 h of incubation.…”

Section: Discussioncontrasting

confidence: 78%

The Influence of Curcumin, Quercetin, and Eicosapentaenoic Acid on the Expression of Phase II Detoxification Enzymes in the Intestinal Cell Lines HT-29, Caco-2, HuTu 80, and LT97

Odenthal

Heumen

Roelofs

et al. 2012

Nutrition and Cancer

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Curcumin, quercetin, and eicosapentaenoic acid (EPA) are 3 natural compounds with the capacity to reduce adenoma burden in patients with familial adenomatous polyposis (FAP). The mechanistic basis of this anticarcinogenic capacity is largely unknown, but it was suggested that induction of detoxification enzymes is involved. Therefore, the effects of low-dose curcumin, quercetin, and EPA on phase II detoxification enzymes UDP-glucuronosyltransferase (UGT), glutathione S-transferase (GST), as well as on glutathione (GSH) content were analyzed in 4 cell line models of intestinal carcinogenesis. HT-29, HuTu 80, and Caco-2 intestinal cancer cells and LT97 colon adenoma cells from a patient with FAP were treated with low-dose noncytotoxic concentrations of curcumin, quercetin, and EPA. GST enzyme activity was measured by spectrophotometry, and expression of GSTA1, GSTM1, GSTP1, GSTT1, and UGT1 by Western blotting. Cytosolic GSH levels were determined by high performance liquid chromatography. An inducing effect of curcumin and quercetin on GST or UGT was seen in Caco-2, LT97, and HuTu 80 cells. GSH levels were reduced by quercetin and EPA in HT-29 cells and induced by curcumin in Caco-2 cells. In LT97 cells, GST activity and expression was reduced, but UGT1 expression was induced by curcumin and quercetin; whereas EPA only decreased GST or UGT levels. In summary, enhancement of the detoxification capacity by low dose of the potential anticarcinogens curcumin, quercetin, or EPA seems only a minor factor in explaining their anticarcinogenic properties.

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Section: Discussioncontrasting

confidence: 78%

The Influence of Curcumin, Quercetin, and Eicosapentaenoic Acid on the Expression of Phase II Detoxification Enzymes in the Intestinal Cell Lines HT-29, Caco-2, HuTu 80, and LT97

Odenthal

Heumen

Roelofs

et al. 2012

Nutrition and Cancer

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“…For this study, relevant concentrations of nonesterified FAs dissolved in ethanol as suitable solvent for in vitro ‐studies [39] were used and it was shown, that LT97 and HT29 cells are able to accumulate FAs, as the amount of FAs measurable within the cells increased (Table 1). This is an important finding as the modification of cell FA composition has been shown to affect signaling pathways [40], lipid peroxidation and oxidative stress [41], gene expression [42], and eicosanoid synthesis [43]. The minor differences of measured FAs can be explained by different utilization and metabolism of the FAs in the cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of fatty acids on metabolism and cell growth of human colon cell lines of different transformation state

et al. 2009

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Epidemiological studies suggest that high fish intake is associated with a decreased risk of colorectal cancer which has been linked to the high content of the n - 3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in some fish. In this study, two different cell lines are compared in relation to their response to EPA and DHA versus the plant derived PUFAs, linoleic acid (LA), gamma-linolenic acid (GLA), and alpha-linolenic acid (ALA) and to the ubiquitous arachidonic acid (ARA). The uptake of 100 microM of each fatty acid (FA) was determined using GC. The 4',6-diamidino-2-phenylindole assay for DNA quantification and the Cell-Titer-Blue assay were used to determine cell survival and metabolic activity at 2-72 h after treatment. All FAs were utilized more efficiently by the human colon adenoma cell line LT97 than by the adenocarcinoma cell line HT29. LT97 were more susceptible than HT29 cells to the growth inhibitory activities of all FAs except for DHA where both were equally sensitive. Inhibition of survival and metabolic activity by EPA and DHA increased with treatment time in both cell lines. ALA or GLA were less growth inhibitory than EPA or DHA and ARA had intermediary activity. The data show that the tested FAs are incorporated into colon cells. Furthermore, adenoma cells are more susceptible than the adenocarcinoma cells.

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“…For these reasons, n-3 PUFAs, particularly DHA, are important in primary health prevention for the general population. Their intake should be in accord with the suggestions based on epidemiological studies 32,33 . Differences in these recommendations reflect distinct experimental approaches, characterization and evaluation of experimental groups.…”

Section: Dietary Intake and Health Benefitsmentioning

confidence: 94%

“…Similarly, cDNA arrays after EPA and DHA treatment using a colon adenoma cell line revealed changes in expression of several gene types, such as those of detoxification, cell cycle control, signalling pathways, apoptosis and inflammation, confirming the chemoprotective effects of these PUFAs (ref. 32 ). Both EPA and DHA decreased the growth of colon tumours in nude mice by reducing vascular endothelial growth factor (VEGF) and COX-2 expression through inhibition of ERK1/2 phosphorylation and hypoxia-induced factor (HIF)-1 α protein expression 108 .…”

Section: Regulation Of Specific Transcription Factors and Dha Effectsmentioning

confidence: 99%

Docosahexaenoic fatty acid (DHA) in the regulation of colon cell growth and cell death: A review

Skender¹,

Vaculová²,

Hofmanová³

2012

BIOMED PAP

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Background. Experimental, epidemiological and clinical data substantiate the beneficial role of n-3 polyunsaturated fatty acids (PUFAs) in preventing inflammation and cancer of the colon. This review covers the unsaturated docosahexaenoic fatty acid (DHA), describes some of its important cellular and molecular mechanisms, its interaction with another dietary lipid, butyrate and with endogenous apoptotic regulators of the tumour necrosis factor (TNF) family. We also discuss the clinical impact of this knowledge and the use of these lipids in colon cancer prevention and treatment. Results. From the literature, DHA has been shown to suppress the growth, induce apoptosis in colon cancer cells in vitro and decrease the incidence and growth of experimental tumours in vivo. Based on these data and our own experimental results, we describe and discuss the possible mechanisms of DHA anticancer effects at various levels of cell organization. We show that DHA can sensitize colon cancer cells to other chemotherapeutic/chemopreventive agents and affect the action of physiological apoptotic regulators of the TNF family. Conclusion. Use of n-3 PUFAs could be a relatively non-toxic form of supportive therapy for improving colon cancer treatment and slowing down or preventing its recurrence. However, it is necessary to use them with caution, based on solid scientific evidence of their mechanisms of action from the molecular to the cellular and organism levels.

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Modulation of gene expression in eicosapentaenoic acid and docosahexaenoic acid treated human colon adenoma cells

Cited by 13 publications

References 10 publications

The Influence of Curcumin, Quercetin, and Eicosapentaenoic Acid on the Expression of Phase II Detoxification Enzymes in the Intestinal Cell Lines HT-29, Caco-2, HuTu 80, and LT97

The Influence of Curcumin, Quercetin, and Eicosapentaenoic Acid on the Expression of Phase II Detoxification Enzymes in the Intestinal Cell Lines HT-29, Caco-2, HuTu 80, and LT97

Effects of fatty acids on metabolism and cell growth of human colon cell lines of different transformation state

Docosahexaenoic fatty acid (DHA) in the regulation of colon cell growth and cell death: A review

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