Modulation of LPS-induced nitric oxide production in intestinal cells by hydroxytyrosol and tyrosol metabolites: Insight into the mechanism of action

Serreli, Gabriele; Melis, Maria Paola; Corona, Giulia; Deiana, Monica

doi:10.1016/j.fct.2019.01.039

Cited by 41 publications

(41 citation statements)

References 67 publications

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“…Recently, our research group showed, in Caco-2 differentiated as normal enterocytes, that glucuronide and sulfate metabolites of HT and Tyr, together with the free forms, were capable to counteract the LPS-induced release of NO. All the tested metabolites suppressed NO release, functioning as inhibitors of iNOS expression following inhibition of NF-κB activation through IkBα phosphorylation and modulation of MAPKs [88]. In the same cell model, we also observed that EVOO phenolic extracts were able to limit oxysterols-mediated NO and cytokines overproduction, by modulating MAPK-NF-κB pathway and consequently iNOS expression [99].…”

Section: Inos Modulationsupporting

confidence: 52%

“…These effects have been noticed in particular in those cells treated with oleuropein aglycone [105], Tyr [98,106], and HT [104], as well as complex phenolic extracts [107,108]. At the intestinal level, the breakdown of the NF-κB inhibitor IkBα was counteracted by EVOO phenolic extracts or by simple phenols HT and Tyr glucuronide and sulfates in intestinal Caco-2 cells, where NF-κB was experimentally activated by a mixture of dietary oxysterols [99] or by LPS [88]. Pretreatment of colon cancer HT-29 cells with HT was also successful in the decrease of NF-kB expression by modulating Akt/PKB and ERK 1/2, which was induced by TNF-α [103].…”

Section: Modulatory Function Of Evoo Polyphenols On Nf-κb Expression mentioning

confidence: 98%

“…Moreover, the catecholic structure of some of them (namely oleuropein, HT and derivatives, for example) may also prevent reactive species formation via metal chelation features. They may counteract the formation of a broad range of ROS (O 2 − above all) and RNS, like the peroxynitrite anion (ONOO − ) [87][88][89]. Beyond direct oxidant effects, ROS play an essential role in intracellular signaling pathways modulation and activity of manifold transcription factors, including NF-κB and activator protein 1 (AP-1) and hypoxia-inducible factor-1α (HIF-1α) [90].…”

Section: Modulatory Function Of Evoo Polyphenols On Nf-κb Expression mentioning

confidence: 99%

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Extra Virgin Olive Oil Polyphenols: Modulation of Cellular Pathways Related to Oxidant Species and Inflammation in Aging

Serreli

Deiana

2020

Cells

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The olive-oil-centered Mediterranean diet has been associated with extended life expectancy and a reduction in the risk of age-related degenerative diseases. Extra virgin olive oil (EVOO) itself has been proposed to promote a “successful aging”, being able to virtually modulate all the features of the aging process, because of its great monounsaturated fatty acids content and its minor bioactive compounds, the polyphenols above all. Polyphenols are mostly antioxidant and anti-inflammatory compounds, able to modulate abnormal cellular signaling induced by pro-inflammatory stimuli and oxidative stress, as that related to NF-E2-related factor 2 (Nrf-2) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), which have been identified as important modulators of age-related disorders and aging itself. This review summarizes existing literature about the interaction between EVOO polyphenols and NF-κB and Nrf-2 signaling pathways. Reported studies show the ability of EVOO phenolics, mainly hydroxytyrosol and tyrosol, to activate Nrf-2 signaling, inducing a cellular defense response and to prevent NF-κB activation, thus suppressing the induction of a pro-inflammatory phenotype. Literature data, although not exhaustive, indicate as a whole that EVOO polyphenols may significantly help to modulate the aging process, so tightly connected to oxidative stress and chronic inflammation.

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Section: Inos Modulationsupporting

confidence: 52%

Section: Modulatory Function Of Evoo Polyphenols On Nf-κb Expression mentioning

confidence: 98%

Section: Modulatory Function Of Evoo Polyphenols On Nf-κb Expression mentioning

confidence: 99%

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Extra Virgin Olive Oil Polyphenols: Modulation of Cellular Pathways Related to Oxidant Species and Inflammation in Aging

Serreli

Deiana

2020

Cells

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“…As to the anti-inflammatory activity, 3-epilupeol (3) shows a marked inhibition of the edema induced by 12- O -tetradecanoylphorbol-13-acetate (TPA) in mice and exhibits nitric oxide (NO) production inhibitory activity in LPS-activated macrophages [ 54 , 55 , 56 , 57 ]. Nitric oxide (NO) is a short-lived bioactive molecule that plays an important role in the host defense response against various pathogens, such as bacteria, viruses, fungi, and parasites, and, also, participates in pathophysiological processes such as neuronal communication, vasodilatation, and neurotoxicity [ 58 , 59 ].…”

Section: Resultsmentioning

confidence: 99%

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Sánchez-Ramos

Álvarez

Romero-Estrada

et al. 2020

Plants

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Ageratina pichinchensis (Kunth) is a plant used in traditional Mexican medicine to treat multiple ailments. However, there have not been biotechnological studies on producing compounds in in vitro cultures. The aim of this study was to establish a cell suspension culture of A. pichinchensis, quantify the anti-inflammatory constituents 2,3-dihydrobenzofuran (2) and 3-epilupeol (3), evaluate the anti-inflammatory potential of its extracts, and perform a phytochemical analysis. Cell suspension cultures were established in a MS culture medium of 30-g L−1 sucrose, 1.0-mg L−1 α-naphthaleneacetic acid, and 0.1-mg L−1 6-furfurylaminopurine. The ethyl acetate extract of the cell culture analyzed by gas chromatography (GC) revealed that the maximum production of anti-inflammatory compounds 2 and 3 occurs on days eight and 16, respectively, improving the time and previously reported yields in callus cultures. The anti-inflammatory activity of these extracts exhibited a significant inhibition of nitric oxide (NO) production. Furthermore, a phytochemical study of the ethyl acetate (EtOAc) and methanol (MeOH) extracts from day 20 led to the identification of 17 known compounds. The structures of the compounds were assigned by an analysis of 1D and 2D NMR data and the remainder by GC–MS. This is the first report of the production of (-)-Artemesinol, (-)-Artemesinol glucoside, encecalin, and 3,5-diprenyl-acetophenone by a cell suspension culture of A. pichinchensis.

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“…Extra virgin olive oil (EVOO), the major source of fatty acids in the traditional Mediterranean diet, is rich in phenolic compounds, such as secoiridoid derivatives of 2‐(3,4‐dihydroxyphenyl)ethanol (hydroxytyrosol, HTy) and hydroxytyrosyl acetate (HTy‐Ac), which have a wide range of anti‐inflammatory, anti‐oxidant, and chemopreventive function in vitro . These beneficial properties were confirmed in vivo in animal models of autoimmunity …”

Section: Introductionmentioning

confidence: 97%

Olive‐Oil‐Derived Polyphenols Effectively Attenuate Inflammatory Responses of Human Keratinocytes by Interfering with the NF‐κB Pathway

Aparicio‐Soto

Redhu

Sánchéz-Hidalgo

et al. 2019

Molecular Nutrition Food Res

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Scope: Extra virgin olive oil (EVOO) is rich in phenolic compounds, including hydroxytyrosol (HTy) and hydroxytyrosyl acetate (HTy-Ac), which have presented multiple beneficial properties. Their impact on inflammatory responses in human keratinocytes and modes of action have not been addressed yet. Methods and results: Primary human keratinocytes are pretreated with HTy-Ac or HTy for 30 min and stimulated with IL-1 or Toll-like receptor 3 ligand (TLR3-l). Thymic stromal lymphopoietin (TSLP), measured by ELISA, is attenuated by both polyphenols in a dose-dependent manner. The expression of several inflammation-related genes, including distinct TSLP isoforms and IL-8, are assessed by quantitative RT-PCR and likewise inhibited by HTy-Ac/HTy. Mechanistically, EVOO phenols counteracts I B degradation and translocation of NF-B to the nucleus, a transcription factor of essential significance to TSLP and IL-8 transcriptional activity; this is evidenced by immunoblotting. Accordingly, NF-B recruitment to critical binding sites in the TSLP and IL-8 promoter is impeded in the presence of HTy-Ac/HTy, as demonstrated by chromatin immunoprecipitation. Promoter reporter assays finally reveal that the neutralizing effect on NF-B induction has functional consequences, resulting in reduced NF-B-directed transcription.

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Modulation of LPS-induced nitric oxide production in intestinal cells by hydroxytyrosol and tyrosol metabolites: Insight into the mechanism of action

Cited by 41 publications

References 67 publications

Extra Virgin Olive Oil Polyphenols: Modulation of Cellular Pathways Related to Oxidant Species and Inflammation in Aging

Extra Virgin Olive Oil Polyphenols: Modulation of Cellular Pathways Related to Oxidant Species and Inflammation in Aging

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Olive‐Oil‐Derived Polyphenols Effectively Attenuate Inflammatory Responses of Human Keratinocytes by Interfering with the NF‐κB Pathway

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