Modulation of Nuclear Factor E2-related Factor-2 (Nrf2) Activation by the Stress Response Gene Immediate Early Response-3 (IER3) in Colonic Epithelial Cells

Stachel, Imke; Geismann, Claudia; Aden, Konrad; Deisinger, Florian; Rosenstiel, Philip; Schreiber, Stefan; Sebens, Susanne; Arlt, Alexander; Schäfer, Heiner

doi:10.1074/jbc.m113.490920

Cited by 49 publications

(24 citation statements)

References 57 publications

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“…IER3-deficient NCM460 cells exhibited reduced reactive oxygen species levels, indicating increased antioxidative protection [50]. In our study, IER3 was down regulated in the VE group suggesting an increase in antioxidative protection.…”

Section: Discussionmentioning

confidence: 47%

Genome-wide expression profiling in muscle and subcutaneous fat of lambs in response to the intake of concentrate supplemented with vitamin E

et al. 2017

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BackgroundThe objective of this study was to acquire a broader, more comprehensive picture of the transcriptional changes in the L. Thoracis muscle (LT) and subcutaneous fat (SF) of lambs supplemented with vitamin E. Furthermore, we aimed to identify novel genes involved in the metabolism of vitamin E that might also be involved in meat quality. In the first treatment, seven lambs were fed a basal concentrate from weaning to slaughter (CON). In the second treatment, seven lambs received basal concentrate from weaning to 4.71 ± 2.62 days and thereafter concentrate supplemented with 500 mg dl-α-tocopheryl acetate/kg (VE) during the last 33.28 ± 1.07 days before slaughter.ResultsThe addition of vitamin E to the diet increased the α-tocopherol muscle content and drastically diminished the lipid oxidation of meat. Gene expression profiles for treatments VE and CON were clearly separated from each other in the LT and SF. Vitamin E supplementation had a dramatic effect on subcutaneous fat gene expression, showing general up-regulation of significant genes, compared to CON treatment. In LT, vitamin E supplementation caused down-regulation of genes related to intracellular signaling cascade. Functional analysis of SF showed that vitamin E supplementation caused up-regulation of the lipid biosynthesis process, cholesterol, and sterol and steroid biosynthesis, and it down-regulated genes related to the stress response.ConclusionsDifferent gene expression patterns were found between the SF and LT, suggesting tissue specific responses to vitamin E supplementation. Our study enabled us to identify novel genes and metabolic pathways related to vitamin E metabolism that might be implicated in meat quality. Further exploration of these genes and vitamin E could lead to a better understanding of how vitamin E affects the oxidative process that occurs in manufactured meat products.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3405-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 47%

Genome-wide expression profiling in muscle and subcutaneous fat of lambs in response to the intake of concentrate supplemented with vitamin E

et al. 2017

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“…Furthermore, a short pilot study was done on 12 children treated with IFN leading to small but significant increase of FXN expression and enhancement of the Friedreich Ataxia Rating Scale after 12 weeks of treatment [49]. Interestingly, IER3, another interferon-stimulated genes, found to be down-regulated in our gene list, has been shown to modulate Nrf2 expression in stress response [50]. Nrf2, anti-oxidative transcription factor, has been reported to be impaired in FRDA patient cells thus leading to cell hypersensitivity and oxidative insults [51].…”

Section: Accepted Manuscriptmentioning

confidence: 94%

Characterization of frataxin gene network in Friedreich's ataxia fibroblasts using the RNA-Seq technique

et al. 2016

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“…Another immune modulator is the immediate early response protein IER3, being associated with inflammatory stress and inflammatory diseases. 45,46 In addition, it is conserved in rodents and humans, where it enhances apoptosis upon DNA damage, p53 activation or diverse stimuli such as TNFα, TRAIL or irradiation.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive identification of genes driven by ERV9-LTRs reveals TNFRSF10B as a re-activatable mediator of testicular cancer cell death

Beyer

Krönung

Leha³

et al. 2015

Cell Death Differ

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The long terminal repeat (LTR) of human endogenous retrovirus type 9 (ERV9) acts as a germline-specific promoter that induces the expression of a proapoptotic isoform of the tumor suppressor homologue p63, GTAp63, in male germline cells. Testicular cancer cells silence this promoter, but inhibitors of histone deacetylases (HDACs) restore GTAp63 expression and give rise to apoptosis. We show here that numerous additional transcripts throughout the genome are driven by related ERV9-LTRs. 3' Rapid amplification of cDNA ends (3'RACE) was combined with next-generation sequencing to establish a large set of such mRNAs. HDAC inhibitors induce these ERV9-LTR-driven genes but not the LTRs from other ERVs. In particular, a transcript encoding the death receptor DR5 originates from an ERV9-LTR inserted upstream of the protein coding regions of the TNFRSF10B gene, and it shows an expression pattern similar to GTAp63. When treating testicular cancer cells with HDAC inhibitors as well as the death ligand TNF-related apoptosis-inducing ligand (TRAIL), rapid cell death was observed, which depended on TNFRSF10B expression. HDAC inhibitors also cooperate with cisplatin (cDDP) to promote apoptosis in testicular cancer cells. ERV9-LTRs not only drive a large set of human transcripts, but a subset of them acts in a proapoptotic manner. We propose that this avoids the survival of damaged germ cells. HDAC inhibition represents a strategy of restoring the expression of a class of ERV9-LTR-mediated genes in testicular cancer cells, thereby re-enabling tumor suppression.

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Modulation of Nuclear Factor E2-related Factor-2 (Nrf2) Activation by the Stress Response Gene Immediate Early Response-3 (IER3) in Colonic Epithelial Cells

Cited by 49 publications

References 57 publications

Genome-wide expression profiling in muscle and subcutaneous fat of lambs in response to the intake of concentrate supplemented with vitamin E

Genome-wide expression profiling in muscle and subcutaneous fat of lambs in response to the intake of concentrate supplemented with vitamin E

Characterization of frataxin gene network in Friedreich's ataxia fibroblasts using the RNA-Seq technique

Comprehensive identification of genes driven by ERV9-LTRs reveals TNFRSF10B as a re-activatable mediator of testicular cancer cell death

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