Modulation of the immune response using Rapana thomasiana hemocyanin

Enhanced structural stability of Concholepas hemocyanin increases its immunogenicity and maintains its non‐specific immunostimulatory effects

“…However, our studies evaluating the immunosti-mulatory properties of the isolated subunits of CCH have shown that the large size and the complex quaternary structure do not determine the immunogenicity and antitumor effects of hemo-cyanins in a mouse model of SBC [12]. This observation has been supported by another report that assessed the immunogenicity of subunits isolated from R. thomasiana hemocyanin [13].…”

Marine gastropod hemocyanins as adjuvants of non-conjugated bacterial and viral proteins

“…In previous experiments we have used higher doses of RtH for immunization (250 mg/mouse) and we observed the animals for six months [19]. There weren't any side effects, IgE synthesis or life span changes detected (data not shown).…”

“…In our previous studies on the hemocyanin isolated from Rapana thomasiana e a marine gastropod living along the west coast of the Black Sea e we demonstrated its high immunogenicity as a single model antigen and also its properties as a strong protein carrier for viral peptides from Influenza hemagglutinin [19]. These results suggested a potential role of RtH as an acceptable compound needed for adjuvanicity of standard vaccines without covalent binding to the antigen.…”

Purification of Hemocyanin from Marine GastropodRapana Thomasianausing Ammonium Sulfate Precipitation Method

“…Rapana thomasiana hemocyanin (Rth) has been structurally well characterized (2,3,6,7,8,9,10,12,14,15,16). Recently, we demonstrated the high immunogenicity of Rth as a model antigen and also its properties as a strong protein carrier for viral peptides from Influenza hemagglutinin (17). The results obtained suggested a potential role of Rth as an acceptable compound needed for adjuvanicity for standard vaccines.…”