Modulatory effects of persimmon vinegar and its fractions on hepatic immune response and CYP2E1 activation in chronically alcohol drinking rats (649.4)

Abstract

Our previous study has shown that persimmon vinegar (PV) had more potent effects on the prevention of the alcohol‐induced liver damage in vivo than any other vinegars tested. Here, we investigated the efficacy of PV and its fractions on hepatic immune response and alcohol metabolism in chronically alcohol drinking rats. Forty male Wistar rats were divided to 5 groups and fed each diet for 4 weeks; normal control, ethanol control (EC), PV, high molecular weight fraction (HPV), and low molecular weight fraction (LPV) group. Hepatic mRNA expressions of Interlekin‐1β, Interlekin‐12β, Toll‐like receptor 4 ,cyclooxygenase‐2 and protein expression of CYP2E1 were significantly decreased in PV, HPV and LPV compared to EC. (p=0.0076,p=0.0009, p=0.0142, p=0.0003, and p=0.0438, respectively). However, there was no significant difference among all treatment groups for all biomarkers with an exception of CYP2E1. The PV had significantly higher potency in suppressing CYP2E1 protein expression than the others. Taken together, the results of this study support the previous observations on the protective effect of PV against alcohol‐induced liver damage. Our findings also suggest that the effect of PV might be caused by more than two components, which are different with their molecular weight range. Grant Funding Source: This study was supported by the Ministry of Education, Science, and Technology (Brain Korea 21 PLUS: