Molecular analysis and bioinformatic characterization of cooper, zinc-superoxide dismutase (Cu/Zn-sod) gene of Caiman latirostris

Paravani, Enrique V.; Odetti, Lucía Magdalena; Simoniello, Ma. Fernanda; Poletta, Gisela Laura

doi:10.1007/s11033-020-05937-y

Cited by 9 publications

(3 citation statements)

References 44 publications

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“…The antioxidative enzyme system (including T-SOD, CuZn-SOD, GSH-Px, CAT, and T-AOC) acts as the first line of defense against antioxidant [ 18 ]. Altering the activity of these enzymes can change the balance between ROS production and the antioxidant system [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Mechanisms Underlying the Protective Effect of Maternal Zinc (ZnSO4 or Zn-Gly) against Heat Stress-Induced Oxidative Stress in Chicken Embryo

Zhang¹,

Xie

Ding

et al. 2022

Antioxidants

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Environmental factors such as high temperature can cause oxidative stress and negatively affect the physiological status and meat quality of broiler chickens. The study was conducted to evaluate the effects of dietary maternal Zn-Gly or ZnSO4 supplementation on embryo mortality, hepatocellular mitochondrial morphology, liver antioxidant capacity and the expression of related genes involved in liver oxidative mechanisms in heat-stressed broilers. A total of 300 36-week-old Lingnan Yellow broiler breeders were randomly divided into three treatments: (1) control (basal diet, 24 mg zinc/kg); (2) inorganic ZnSO4 group (basal diet +80 mg ZnSO4/kg); (3) organic Zn-Gly group (basal diet +80 mg Zn-Gly/kg). The results show that maternal zinc alleviated heat stress-induced chicken embryo hepatocytes’ oxidative stress by decreasing the content of ROS, MDA, PC, 8-OHdG, and levels of HSP70, while enhancing T-SOD, T-AOC, CuZn-SOD, GSH-Px, CTA activities and the content of MT. Maternal zinc alleviated oxidative stress-induced mitochondrial damage in chick embryo hepatocytes by increasing mitochondrial membrane potential and UCP gene expression; and Caspase-3-mediated apoptosis was alleviated by increasing CuZn-SOD and MT gene expression and decreasing Bax gene expression and reducing the activity of caspase 3. Furthermore, maternal zinc treatment significantly increased Nrf2 gene expression. The results above suggest that maternal zinc can activate the Nrf2 signaling pathway in developing chick embryos, enhance its antioxidant function and reduce the apoptosis-effecting enzyme caspase-3 activities, thereby slowing oxidative stress injury and tissue cell apoptosis.

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Section: Discussionmentioning

confidence: 99%

Mechanisms Underlying the Protective Effect of Maternal Zinc (ZnSO4 or Zn-Gly) against Heat Stress-Induced Oxidative Stress in Chicken Embryo

Zhang¹,

Xie

Ding

et al. 2022

Antioxidants

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show abstract

“…To solve those issues, researchers have attempted to modify or mutate SODs, or search for SODs with excellent performance. SODs with superior properties have been successfully characterized from various organisms, such as the SODs of Caiman latirostris [ 13 ], Zostera marina [ 14 ], and Acipenser baerii [ 15 ]. In recent years, deep-sea biomes have attracted the attention of researchers.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus)

Chen

Zhang

et al. 2023

Antioxidants

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At present, deep-sea enzymes are a research hotspot. In this study, a novel copper–zinc superoxide dismutase (CuZnSOD) was successfully cloned and characterized from a new species of sea cucumber Psychropotes verruciaudatus (PVCuZnSOD). The relative molecular weight of the PVCuZnSOD monomer is 15 kDa. The optimum temperature of PVCuZnSOD is 20 °C, and it maintains high activity in the range of 0–60 °C. It also has high thermal stability when incubated at 37 °C. PVCuZnSOD has a maximum activity of more than 50% in the pH range of 4–11 and a high activity at pH 11. In addition, PVCuZnSOD has strong tolerance to Ni2+, Mg2+, Ba2+, and Ca2+, and it can withstand chemical reagents, such as Tween20, TritonX-100, ethanol, glycerol, isopropanol, DMSO, urea, and GuHCl. PVCuZnSOD also shows great stability to gastrointestinal fluid compared with bovine SOD. These characteristics show that PVCuZnSOD has great application potential in medicine, food, and other products.

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“…There are some antioxidant systems that maintain dynamic balance of ROS in aquatic species. Superoxide dismutase (SOD) family, the rst line of defense to eliminate ROS, can catalyze ROS into O 2 and H 2 O 2 [11,12]. Then, H 2 O 2 is further catalyzed to H 2 O and O 2 by peroxiredoxin, catalases and peroxidases [13].…”

Section: Introductionmentioning

confidence: 99%

An extracellular Cu/Zn superoxide dismutase from Neocaridina denticulata sinensis: cDNA cloning, mRNA expression and characterizations of recombinant protein

Feng¹,

Gao²,

Kong³

et al. 2022

Fish & Shellfish Immunology

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Molecular analysis and bioinformatic characterization of cooper, zinc-superoxide dismutase (Cu/Zn-sod) gene of Caiman latirostris

Cited by 9 publications

References 44 publications

Mechanisms Underlying the Protective Effect of Maternal Zinc (ZnSO4 or Zn-Gly) against Heat Stress-Induced Oxidative Stress in Chicken Embryo

Mechanisms Underlying the Protective Effect of Maternal Zinc (ZnSO4 or Zn-Gly) against Heat Stress-Induced Oxidative Stress in Chicken Embryo

Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus)

An extracellular Cu/Zn superoxide dismutase from Neocaridina denticulata sinensis: cDNA cloning, mRNA expression and characterizations of recombinant protein

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