Molecular analysis and genetic diversity of
            <i>Aedes albopictus</i>
            (Diptera, Culicidae) from China

Zhang, Ruiling; PeiEn, Leng; Wang, Xuejun; Zhong, Zhang

doi:10.1080/24701394.2017.1325481

Cited by 14 publications

(14 citation statements)

References 28 publications

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“…The same result for Chinese Ae. albopictus populations was also observed by both Guo et al and Zhang et al [79,80]. Guo dispersal from neighboring Southeast Asian countries may be the reason for the higher diversity of the Ae.…”

Section: Discussionsupporting

confidence: 53%

Temporal genetic variation and dispersal patterns of Aedes albopictus (Diptera: Culicidae) in three different climatic regions of China

Zhang¹,

X²,

Xing³

et al. 2020

Preprint

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Background:Aedes albopictus is an indigenous primary vector for Dengue and Zika viruses in China. Compared with its insecticide resistance, biology, and vector competence, little is known about its genetic variation, which corresponds to environmental variations. Thus, the present study examines how Ae. albopictus varies among different climatic regions in China and deciphers its potential dispersal patterns.Methods:The genetic variation and population structure of 17 Ae. albopictus populations collected from three climatic regions of China were investigated with 11 microsatellite loci and the mitochondrial COI gene.Results:Of 44 isolated microsatellite markers, 11 pairs were chosen for genotyping analysis and had an average PIC value of 0.713, representing high polymorphism. The number of alleles was high in each population, with the ne value increasing from the temperate region (3.876) to the tropical region (4.144). Twenty-five COI haplotypes were detected, and the highest diversity was observed in the tropical region. The mean Ho value (ca. 0.557) of all the regions was significantly lower than the mean He value (ca. 0.684), with nearly all populations significantly departing from HWE and displaying significant population expansion (p-value < 0.05). Two genetically isolated groups and three haplotype clades were evaluated via STRUCTURE and haplotype phylogenetic analyses, and the tropical populations were significantly isolated from those in the other regions. Most genetic variation in Ae. albopictus was detected within populations and individuals at 31.40% and 63.04%, respectively, via the AMOVA test, and a relatively significant positive correlation was observed among only the temperate populations via IBD analysis (R2 = 0.6614, p = 0.048). Recent dispersions were observed among different Ae. albopictus populations, and four major migration trends with high gene flow (Nm>0.4) were reconstructed between the tropical region and the other two regions. Environmental factors, especially temperature and rainfall, may be the leading causes of genetic diversity in different climatic regions.Conclusions:Continuous dispersion contributes to the similarity of Ae. albopictus populations across different climatic regions, and environmental factors, especially temperature and rainfall, may be the leading causes of genetic variation.

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Section: Discussionsupporting

confidence: 53%

Temporal genetic variation and dispersal patterns of Aedes albopictus (Diptera: Culicidae) in three different climatic regions of China

Zhang¹,

X²,

Xing³

et al. 2020

Preprint

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“…The same result for Chinese Ae. albopictus populations was also observed by both Guo et al and Zhang et al [79,80]. Guo et al identi ed three predominant haplotypes in Ae.…”

Section: Discussionmentioning

confidence: 64%

Dispersal patterns and population structure of Aedes albopictus (Diptera: Culicidae) in three different climatic regions of China

Gao

Zhang

Guo

et al. 2020

Preprint

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Background:Aedes albopictus is an indigenous primary vector for Dengue and Zika viruses in China. Compared with its insecticide resistance, biology, and vector competence, little is known about its genetic variation, which corresponds to environmental variations. Thus, the present study examines how Ae. albopictus varies among different climatic regions in China and deciphers its potential dispersal patterns.Methods:The genetic variation and population structure of 17 Ae. albopictus populations collected from three climatic regions of China were investigated with 11 microsatellite loci and the mitochondrial coxI gene.Results:Of 44 isolated microsatellite markers, 11 pairs were chosen for genotyping analysis and had an average PIC value of 0.713, representing high polymorphism. The number of alleles was high in each population, with the ne value increasing from the temperate region (3.876) to the tropical region (4.144). Twenty-five coxI haplotypes were detected, and the highest diversity was observed in the tropical region. The mean Ho value (ca. 0.557) of all the regions was significantly lower than the mean He value (ca. 0.684), with nearly all populations significantly departing from HWE and displaying significant population expansion (p-value < 0.05). Two genetically isolated groups and three haplotype clades were evaluated via STRUCTURE and haplotype phylogenetic analyses, and the tropical populations were significantly isolated from those in the other regions. Most genetic variation in Ae. albopictus was detected within populations and individuals at 31.40% and 63.04%, respectively, via the AMOVA test, and a relatively significant positive correlation was observed among only the temperate populations via IBD analysis (R2 = 0.6614, p = 0.048). Recent dispersions were observed among different Ae. albopictus populations, and four major migration trends with high gene flow (Nm>0.4) were reconstructed between the tropical region and the other two regions. Environmental factors, especially temperature and rainfall, may be the leading causes of genetic diversity in different climatic regions.Conclusions:Continuous dispersion contributes to the genetic communication of Ae. albopictus populations across different climatic regions, and environmental factors, especially temperature and rainfall, may be the leading causes of genetic variation.

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“…Few studies have reported the population structure and genetic diversity of Ae. albopictus in China, and they have focused on using mitochondrial DNA markers to analyze genetic diversity, regional structure, mainly in populations in southern China, or they have used a limited sampling size [31, 55]. For example, Guo et al [31] sequenced the mitochondrial DNA cytochrome c oxidase subunit 1 ( cox 1) gene of 140 Ae.…”

Section: Discussionmentioning

confidence: 99%

“…For example, Guo et al [31] sequenced the mitochondrial DNA cytochrome c oxidase subunit 1 ( cox 1) gene of 140 Ae. albopictus from 14 populations in southern China, and Zhang et al [55] sequenced cox 1 of 119 Ae. albopictus from 17 populations in China.…”

Section: Discussionmentioning

confidence: 99%

Patterns of spatial genetic structures in Aedes albopictus (Diptera: Culicidae) populations in China

Wang

Song

et al. 2019

Parasites Vectors

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BackgroundThe Asian tiger mosquito, Aedes albopictus, is one of the 100 worst invasive species in the world and the vector for several arboviruses including dengue, Zika and chikungunya viruses. Understanding the population spatial genetic structure, migration, and gene flow of vector species is critical to effectively preventing and controlling vector-borne diseases. Little is known about the population structure and genetic differentiation of native Ae. albopictus in China. The aim of this study was to examine the patterns of the spatial genetic structures of native Ae. albopictus populations, and their relationship to dengue incidence, on a large geographical scale.MethodsDuring 2016–2018, adult female Ae. albopictus mosquitoes were collected by human landing catch (HLC) or human-bait sweep-net collections in 34 localities across China. Thirteen microsatellite markers were used to examine the patterns of genetic diversity, population structure, and gene flow among native Ae. albopictus populations. The correlation between population genetic indices and dengue incidence was also examined.ResultsA total of 153 distinct alleles were identified at the 13 microsatellite loci in the tested populations. All loci were polymorphic, with the number of distinct alleles ranging from eight to sixteen. Genetic parameters such as PIC, heterozygosity, allelic richness and fixation index (FST) revealed highly polymorphic markers, high genetic diversity, and low population genetic differentiation. In addition, Bayesian analysis of population structure showed two distinct genetic groups in southern-western and eastern-central-northern China. The Mantel test indicated a positive correlation between genetic distance and geographical distance (R2 = 0.245, P = 0.01). STRUCTURE analysis, PCoA and GLS interpolation analysis indicated that Ae. albopictus populations in China were regionally clustered. Gene flow and relatedness estimates were generally high between populations. We observed no correlation between population genetic indices of microsatellite loci in Ae. albopictus populations and dengue incidence.ConclusionStrong gene flow probably assisted by human activities inhibited population differentiation and promoted genetic diversity among populations of Ae. albopictus. This may represent a potential risk of rapid spread of mosquito-borne diseases. The spatial genetic structure, coupled with the association between genetic indices and dengue incidence, may have important implications for understanding the epidemiology, prevention, and control of vector-borne diseases.

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Molecular analysis and genetic diversity of Aedes albopictus (Diptera, Culicidae) from China

Cited by 14 publications

References 28 publications

Temporal genetic variation and dispersal patterns of Aedes albopictus (Diptera: Culicidae) in three different climatic regions of China

Temporal genetic variation and dispersal patterns of Aedes albopictus (Diptera: Culicidae) in three different climatic regions of China

Dispersal patterns and population structure of Aedes albopictus (Diptera: Culicidae) in three different climatic regions of China

Patterns of spatial genetic structures in Aedes albopictus (Diptera: Culicidae) populations in China

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