Molecular analysis of clinical Citrobacter spp. isolates: Acquisition of the Yersinia high-pathogenicity island mediated by ICEkp in C. freundii

Zhang, Guangcun; Zhao, Qiang; Ye, Kun; Ye, Liyan; Ma, Yue; Yang, Jun

doi:10.3389/fmicb.2023.1056790

Cited by 3 publications

(2 citation statements)

References 28 publications

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“…These studies and our data indicate that the underlying molecular epidemiology and OXA-48 plasmid architecture differs between C. freundii and C. portucalensis. However, C. portucalensis is commonly misidentified as C. freundii by Matrix-assisted laser desorption/ionization (MALDI-TOF) mass spectrometry and is likely underreported for this reason [33]. In one C. portucalensis isolate, we identified a chromosomal copy of bla OXA-48 in addition to an IncL-localised copy.…”

Section: Discussionmentioning

confidence: 93%

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Emergence of OXA-48-like producing Citrobacter species, Germany, 2011 to 2022

Sommer,

Reiter,

Sattler

et al. 2024

Eurosurveillance

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Background Carbapenemase-producing Enterobacterales are a public health threat worldwide and OXA-48 is the most prevalent carbapenemase in Germany and western Europe. However, the molecular epidemiology of OXA-48 in species other than Escherichia coli and Klebsiella pneumoniae remains poorly understood. Aim To analyse the molecular epidemiology of OXA-48 and OXA-48-like carbapenemases in Citrobacter species (spp.) in Germany between 2011 and 2022. Methods Data of 26,822 Enterobacterales isolates sent to the National Reference Centre (NRC) for Gram-negative bacteria were evaluated. Ninety-one Citrobacter isolates from 40 German hospitals harbouring bla OXA-48/OXA-48‑like were analysed by whole genome sequencing and conjugation experiments. Results The frequency of OXA-48 in Citrobacter freundii (CF) has increased steadily since 2011 and is now the most prevalent carbapenemase in this species in Germany. Among 91 in-depth analysed Citrobacter spp. isolates, CF (n = 73) and C. koseri (n = 8) were the most common species and OXA-48 was the most common variant (n = 77), followed by OXA-162 (n = 11) and OXA‑181 (n = 3). Forty percent of the isolates belonged to only two sequence types (ST19 and ST22), while most other STs were singletons. The plasmids harbouring bla OXA‑48 and bla OXA-162 belonged to the plasmid types IncL (n = 85) or IncF (n = 3), and plasmids harbouring bla OXA‑181 to IncX3 (n = 3). Three IncL plasmid clusters (57/85 IncL plasmids) were identified, which were highly transferable in contrast to sporadic plasmids. Conclusion In CF in Germany, OXA-48 is the predominant carbapenemase. Dissemination is likely due to distinct highly transmissible plasmids harbouring bla OXA‑48 or bla OXA-48-like and the spread of the high-risk clonal lineages ST19 and ST22.

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Section: Discussionmentioning

confidence: 93%

“…Given the increase of Citrobacter spp. involved in the carbapenemase dissemination with distinct epidemiological pattern, future surveys should include methods that can distinguish C. portucalensis [32,33].…”

Section: Discussionmentioning

confidence: 99%

Emergence of OXA-48-like producing Citrobacter species, Germany, 2011 to 2022

Sommer,

Reiter,

Sattler

et al. 2024

Eurosurveillance

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AI-2 quorum sensing signal disrupts coral symbiotic homeostasis and induces host bleaching

Han,

Cheng,

Wang

et al. 2024

Environment International

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Multi-Lasso Peptide-Based Synergistic Nanocomposite: A High-Stability, Broad-Spectrum Antimicrobial Agent with Potential for Combined Antibacterial Therapy

Li,

Zhang,

Wei

et al. 2024

ACS Nano

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Lasso peptides, natural biological microcins composed of small molecules, have demonstrated efficient bactericidal activity. However, a single lasso peptide is characterized by a narrow and targeted bactericidal spectrum. In this study, a chitosan (CN) derivative-based polymer nanomaterial incorporating three lasso peptides (MccY, MccJ25, and Klebsidin) was designed and synthesized to broaden its antimicrobial spectrum. To enhance resistance to acid and alkali conditions, arginine was appended to the terminus of conjugates, resulting in Chitosan-Lasso-Peptides-Arg (CN-LPs-Arg), and the nanomaterial biocompatibility and bactericidal activity were characterized. Chemical stability test results demonstrate that CN-LPs-Arg effectively buffered the acid−base effect of the compound. Notably, CN-LPs-Arg extended the antimicrobial spectrum of Gram-negative and Grampositive strains including Klebsiella, Salmonella, and Staphylococcus (MIC = 0.01−1.0 μM). CN-LPs-Arg exerts its destructive effects on bacteria via a series of mechanisms; it adheres to and then penetrates the membrane, causes rupture, and leads to bacterial death. Transcriptomic data revealed that CN-LPs-Arg produced a distinct inhibitory effect on ribosomal protein subunits synthesis pathways and membrane metabolic inhibition. Furthermore, CN-LPs-Arg was nontoxic to cells and exhibited excellent biocompatibility. CN-LPs-Arg reduced bacterial burden in organs and the levels of inflammatory factors IL-6, IL-8, and TNF-α in tissues of mice with acute bacterial infections. Furthermore, it promoted the recovery of Klebsiellainfected C57BL/6 mice, demonstrating a favorable therapeutic effect in vivo. The multilasso peptide-based synergistic nanocomposite of CN-LPs-Arg exhibited high stability as a broad-spectrum antimicrobial agent with potential for combined antibacterial therapy and utilization in the fields of food, biomedicine, and public health.

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Molecular analysis of clinical Citrobacter spp. isolates: Acquisition of the Yersinia high-pathogenicity island mediated by ICEkp in C. freundii

Cited by 3 publications

References 28 publications

Emergence of OXA-48-like producing Citrobacter species, Germany, 2011 to 2022

Emergence of OXA-48-like producing Citrobacter species, Germany, 2011 to 2022

AI-2 quorum sensing signal disrupts coral symbiotic homeostasis and induces host bleaching

Multi-Lasso Peptide-Based Synergistic Nanocomposite: A High-Stability, Broad-Spectrum Antimicrobial Agent with Potential for Combined Antibacterial Therapy

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