Molecular and biochemical rhythms in dihydroflavonol 4-reductase- mediated regulation of leucoanthocyanidin biosynthesis in Carthamus tinctorius L

Ahmad, Naveed; Li, Tian; Liu, Yueyang; Hoang, Nguyen Quoc Viet; Ma, Xintong; Zhang, Xinyue; Liu, Jianyu; Yao, Na; Liu, Xiuming; Li, Haiyan

doi:10.1016/j.indcrop.2020.112838

Cited by 24 publications

(10 citation statements)

References 48 publications

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“…Further classifications revealed that most of the CtF3'H proteins shared an identical pattern of evolutionary pattern with S. miltiorrhiza and V. vinifera, P. hybrida and I. purpurea. These findings were consistent with evolution of plant P450 family as desribed by (Ahmad et al, 2020;Barvkar et al, 2012).…”

Section: Discussionsupporting

confidence: 89%

Genome-wide identification and expression profiling of duplicated flavonoid 3'-hydroxylase gene family in Carthamus tinctorius L.

Hoang

Kong

Ahmad

et al. 2021

Not Bot Horti Agrobo

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Flavonoid 3′-hydroxylase (F3’H) enzyme is essential in determining the flavonoids B-ring hydroxylation pattern. It is mainly implicated in the biosynthetic pathway of cyaniding-based anthocyanins, flavonols, and flavan-3-ols. However, the evolution and regulatory mechanism of these important flavonoid hydroxylases have not been systematically investigated in safflower (Carthamus tinctorius L.). In this study, we identified 22 duplicatedCtF3'H-encoding genes from safflower through genome-wide prediction and conservation analysis. Phylogenetic analysis revealed the pattern of conservation and divergence of CtF3'Hs encoding proteins and their homologs from different plant species. The distribution of conserved protein motifs and cis-regulatory units suggested several structural components that could be crucial in deciphering the final function of CtF3'H proteins. Furthermore, the results of RNA-seq and qRT-PCR assay in different flowering tissues suggested differential expression level of CtF3’H genes during flower development. Based on the unique homology of CtF3’H5 with flavonoid 3’ hydroxylases from other plant species, further validation of CtF3’H5 was carried out. The transient expression of CtF3’H5 in onion epidermal cells implied that the subcellular localization of the fusion construct containing CtF3’H5 and GFP was predominantly detected in the plasma membrane. Similarly, the prokaryotic expression and western blot hybridization of CtF3’H5 demonstrated the detection of a stable 50.3kD target protein. However, more efforts are needed to further extend the functional validation of CtF3’H5 in safflower. This study provides a fundamental gateway for future functional studies and understanding the genetic evolution of F3'Hs in plants.

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Section: Discussionsupporting

confidence: 89%

Genome-wide identification and expression profiling of duplicated flavonoid 3'-hydroxylase gene family in Carthamus tinctorius L.

Hoang

Kong

Ahmad

et al. 2021

Not Bot Horti Agrobo

Self Cite

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“…A previous study suggested that petunia flowers turn red when DFR enzyme catalyze the synthesis of pelargonin [ 51 ]. Recently, the positive role of DFR enzyme has been shown during the effective production of leucoanthocyanidin biosynthesis in safflower [ 52 ]. Pelargonin is accumulated in the petals of transgenic plants when maize DFR gene is introduced into white petunias, resulting in red petals [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptional networks orchestrating red and pink testa color in peanut

Naveed

Zhang

et al. 2023

BMC Plant Biol

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Background Testa color is an important trait of peanut (Arachis hypogaea L.) which is closely related with the nutritional and commercial value. Pink and red are main color of peanut testa. However, the genetic mechanism of testa color regulation in peanut is not fully understood. To elucidate a clear picture of peanut testa regulatory model, samples of pink cultivar (Y9102), red cultivar (ZH12), and two RNA pools (bulk red and bulk pink) constructed from F4 lines of Y9102 x ZH12 were compared through a bulk RNA-seq approach. Results A total of 2992 differential expressed genes (DEGs) were identified among which 317 and 1334 were up-regulated and 225 and 1116 were down-regulated in the bulk red-vs-bulk pink RNA pools and Y9102-vs-ZH12, respectively. KEGG analysis indicates that these genes were divided into significantly enriched metabolic pathways including phenylpropanoid, flavonoid/anthocyanin, isoflavonoid and lignin biosynthetic pathways. Notably, the expression of the anthocyanin upstream regulatory genes PAL, CHS, and CHI was upregulated in pink and red testa peanuts, indicating that their regulation may occur before to the advent of testa pigmentation. However, the differential expression of down-stream regulatory genes including F3H, DFR, and ANS revealed that deepening of testa color not only depends on their gene expression bias, but also linked with FLS inhibition. In addition, the down-regulation of HCT, IFS, HID, 7-IOMT, and I2’H genes provided an alternative mechanism for promoting anthocyanin accumulation via perturbation of lignin and isoflavone pathways. Furthermore, the co-expression module of MYB, bHLH, and WRKY transcription factors also suggested a fascinating transcriptional activation complex, where MYB-bHLH could utilize WRKY as a co-option during the testa color regulation by augmenting anthocyanin biosynthesis in peanut. Conclusions These findings reveal candidate functional genes and potential strategies for the manipulation of anthocyanin biosynthesis to improve peanut varieties with desirable testa color.

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“…Van Boeckel et al [ 1 ] conducted an analysis of antimicrobial consumption in animal production systems, and observed significant differences in average usage rates across species. Specifically, the mean amount of antimicrobial agents consumed per kilogram of animal produced was estimated to be 45 mg/kg for cattle, 148 mg/kg for chickens, and 172 mg/kg for pigs [ 35 ]. Furthermore, antimicrobial dosage pattern dependent on animal life stage.…”

Section: Antibiotics: Types and Concentrations In Livestock Manurementioning

confidence: 99%

The Antibiotics Degradation and Its Mechanisms during the Livestock Manure Anaerobic Digestion

Zubair

Zhu

et al. 2023

Molecules

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Antibiotics are administered to livestock at subtherapeutic levels to promote growth, and their degradation in manure is slow. High antibiotic concentrations can inhibit bacterial activity. Livestock excretes antibiotics via feces and urine, leading to their accumulation in manure. This can result in the propagation of antibiotic-resistant bacteria and antibiotic resistance genes (ARGs). Anaerobic digestion (AD) manure treatment technologies are gaining popularity due to their ability to mitigate organic matter pollution and pathogens, and produce methane-rich biogas as renewable energy. AD is influenced by multiple factors, including temperature, pH, total solids (TS), substrate type, organic loading rate (OLR), hydraulic retention time (HRT), intermediate substrates, and pre-treatments. Temperature plays a critical role, and thermophilic AD has been found to be more effective in reducing ARGs in manure compared to mesophilic AD, as evidenced by numerous studies. This review paper investigates the fundamental principles of process parameters affecting the degradation of ARGs in anaerobic digestion. The management of waste to mitigate antibiotic resistance in microorganisms presents a significant challenge, highlighting the need for effective waste management technologies. As the prevalence of antibiotic resistance continues to rise, urgent implementation of effective treatment strategies is necessary.

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Molecular and biochemical rhythms in dihydroflavonol 4-reductase- mediated regulation of leucoanthocyanidin biosynthesis in Carthamus tinctorius L

Cited by 24 publications

References 48 publications

Genome-wide identification and expression profiling of duplicated flavonoid 3'-hydroxylase gene family in Carthamus tinctorius L.

Genome-wide identification and expression profiling of duplicated flavonoid 3'-hydroxylase gene family in Carthamus tinctorius L.

Transcriptional networks orchestrating red and pink testa color in peanut

The Antibiotics Degradation and Its Mechanisms during the Livestock Manure Anaerobic Digestion

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