Molecular and cellular events during the yeast to mycelium transition in Sporothrix schenckii

Betancourt, Soraya S.; Torres-Bauza, L J; Valle, Nuri Rodrı́guez-del

doi:10.1080/00362178585380311

Cited by 22 publications

(24 citation statements)

References 17 publications

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“…A prerequisite for this transition process is a nuclear division; afterwards, a germ tube is originated from the parental yeast cell and a septum is formed at mother cell-germ tube formation (25). It is interesting to note that yeast cells can be maintained at 25°C if cultured in liquid media with glucose and with the pH around 7.2 (195,260).…”

Section: Vol 24 2011mentioning

confidence: 99%

Sporothrix schenckii and Sporotrichosis

Barros

Paes²,

Schubach³

2011

Clin Microbiol Rev

532

677

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SUMMARY Sporotrichosis, which is caused by the dimorphic fungus Sporothrix schenckii , is currently distributed throughout the world, especially in tropical and subtropical zones. Infection generally occurs by traumatic inoculation of soil, plants, and organic matter contaminated with the fungus. Certain leisure and occupational activities, such as floriculture, agriculture, mining, and wood exploitation, are traditionally associated with the mycosis. Zoonotic transmission has been described in isolated cases or in small outbreaks. Since the end of the 1990s there has been an epidemic of sporotrichosis associated with transmission by cats in Rio de Janeiro, Brazil. More than 2,000 human cases and 3,000 animal cases have been reported. In humans, the lesions are usually restricted to the skin, subcutaneous cellular tissue, and adjacent lymphatic vessels. In cats, the disease can evolve with severe clinical manifestations and frequent systemic involvement. The gold standard for sporotrichosis diagnosis is culture. However, serological, histopathological, and molecular approaches have been recently adopted as auxiliary tools for the diagnosis of this mycotic infection. The first-choice treatment for both humans and cats is itraconazole.

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Section: Vol 24 2011mentioning

confidence: 99%

Sporothrix schenckii and Sporotrichosis

Barros

Paes²,

Schubach³

2011

Clin Microbiol Rev

532

677

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“…These cells were induced to form germ tubes by inoculating them into 50 ml flasks containing 20 ml of basal medium with glucose at pH 4.0 (containing 4 #M calcium) at an initial cell concentration of 2 x 105 cells m1-1 followed by incubation at 25 °C. Previous reports have shown that these culture conditions induce the yeast to mycelium transition with reproducible kinetics in approximately 100% of the cells, 12 h after inoculation [ 3 ]. For experiments requiring the maintenance of the yeast morphology (non-induced yeast cells), yeast cells were inoculated as described above but at pH 7.2 and 35 °C with aeration.…”

Section: Methodsmentioning

confidence: 99%

“…Yeast cells of S. schenckii (ATCC strain number 58251) were harvested, grown and selected by filtration as described previously [3 ]. These cells were induced to form germ tubes by inoculating them into 50 ml flasks containing 20 ml of basal medium with glucose at pH 4.0 (containing 4 #M calcium) at an initial cell concentration of 2 x 105 cells m1-1 followed by incubation at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Sporothrix schenckii is a pathogenic dimorphic fungus that grows in the yeast or mycelium form, depending on the culture conditions [ 1,3 ]. These dimorphic transitions are reversible and offer a suitable model for the study of differentiation in a lower eukaryote.…”

Section: Introductionmentioning

confidence: 99%

“…This process constitutes the first visible morphological evidence of the yeast to mycelium transition. Nevertheless, it is preceded by RNA synthesis, DNA synthesis and nuclear division [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Calcium uptake and efflux during the yeast to mycelium transition inSporothrix schenckii

Serrano

Valle

1990

Mycopathologia

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A study was made of calcium metabolism during germ tube formation in Sporothrix schenckii yeast cells. A net efflux of calcium was observed very early in the transformation process and remained constant thereafter. The efflux of calcium in yeast cells induced to form germ tubes was twice that observed in yeast cells not induced to form germ tubes. Two peaks of calcium uptake were observed in germ tube forming yeast cells at 30 and 300 minutes following inoculation, while non-induced yeast cells, a continuous increase in uptake was observed which ultimately reached higher values than the ones obtained in germ tube forming cells. Substances which affect calcium metabolism in other cells such as cobalt ions, ionophore A23187 and compound R24571 were observed to inhibit germ tube formation and calcium uptake. In addition, ionophore A23187 was found to increase calcium efflux to approximately twice the control values. The inhibition of germ tube formation brought about by substances which inhibit calcium uptake or increase efflux suggests that the intracellular calcium concentration in these cells must be precisely regulated for the yeast to mycelium transition to occur.

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Studies on Phase Transitions in Sporothrix Schenckii: Possible Involvement of Protein Kinase C

Colon-Colon

Valle

1993

Dimorphic Fungi in Biology and Medicine

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Molecular and cellular events during the yeast to mycelium transition in Sporothrix schenckii

Cited by 22 publications

References 17 publications

Sporothrix schenckii and Sporotrichosis

Sporothrix schenckii and Sporotrichosis

Calcium uptake and efflux during the yeast to mycelium transition inSporothrix schenckii

Studies on Phase Transitions in Sporothrix Schenckii: Possible Involvement of Protein Kinase C

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