Molecular and Immunological test for detection of Toxoplasma gondii in pregnant women and patients with prostate cancer

Abstract

A cross-sectional study had achieved in Baghdad government from March 2016 to March 2017". 100 patients with prostatic cancer were involved in this study. The age of the patients was between (30-75) years, control bunch who were coordinated to the patients, including100 pregnant patients and 100 solid people (blood givers). Five ml of blood have gathered from every patient, and the control gathering took a crack at this examination. Blood tests were set into two cylinders; one of the tubes was contained anticoagulant EDTA for an atomic trial of Toxoplasma gondii, another tube was 2 ml have centrifuged. "The obtained sera was aspirated, and stored for identification of specific Toxoplasma gondii IgM and IgG by using ELISA technique". "The study found that 40% of prostate cancer patients were positive to T. gondii by RT-PCR compared with 30% in pregnant patients and 13% of non-diseased (control). The study revealed that 55% of patients with cancer of prostate with result +ve ELISA was also +ve by PCR compared with 0% of patients with -ve ELISA results, Current study that appearance 60% of cancer of prostate patients with T. gondii IgM+ IgG+ and 58.33% IgM+ IgG-antibodies were positive by PCR. "Our study revealed that the high rate of T. gondii infection" "was founded among patients with prostatic cancer older than 70 years (53.86%) and low rate" (20%) was within patients between (30-39) year and we were observed, "That Toxoplasma gondii infection increased proportionally with increase of patients age. In conclusion, a high rate of prevalence of T. gondii DNA (as detected by RT-PCR), as well as its antibodies, have observed in prostate cancer and RT PCR was the precise manner in the detection of T.gondii.