Molecular and Microbial Signatures Predictive of Prebiotic Action of Neoagarotetraose in a Dextran Sulfate Sodium-Induced Murine Colitis Model

Abstract

<jats:p>Neoagarotetraose (NT), a hydrolytic product of agar by β-agarase, is known to possess bioactive properties. However, the mechanisms via which NT alleviates intestinal inflammation remain unknown. In this study, a dextran sulfate sodium (DSS)-induced murine model was developed to evaluate the effect of NT on gut microbiome and microbial metabolism using 16S rRNA gene sequencing and untargeted metabolomics. Our data demonstrate that NT ingestion improved gut integrity and inflammation scores. NT reversed the abundance of Proteobacteria from an elevated level induced by DSS and significantly increased the abundance of Verrucomicrobia. Further, NT significantly increased the abundance of Akkermansia and Lactobacillus and concomitantly decreased that of Sutterella, which were among the important features identified by random forests analysis contributing to classification accuracy for NT supplementation. A microbial signature consisting of Adlercreutzia (denominator) and Turicibacter (numerator) predicted the NT supplementation status. Moreover, NT significantly modulated multiple gut metabolites, particularly those related to histidine, polyamine and tocopherol metabolism. Together, our findings provided novel insights into the mechanisms by which NT modulated the gut microbiome and metabolome and should facilitate the development of NT as a potent prebiotic for colitis management.</jats:p>

Shifts in Intestinal Metabolic Profile Among Kidney Transplantation Recipients with Antibody-Mediated Rejection

“…There is growing evidence of roles of intestinal metabolites as biomarkers of human diseases such as colorectal cancer, 28 colitis, 29 irritable bowel syndrome, 30 and Parkinson’s disease. 31 In this study, N-Palmitoylsphingosine, Methylguanidine and Erucamide were found to have satisfactory discriminative power to distinguish the KT-AMR and ESRD groups.…”

Non-targeted metabolomics of cooked cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan) from Ghana using two distinct and complementary analytical platforms

“…N,N-dimethylalanine is another pigeon pea metabolite that has not been extensively characterized, but this compound had been previously investigated to characterize the mechanism in which neoagarotetraose alleviates intestinal inflammation. The N,N-dimethylalanine pathway was suggested to play a role in the prebiotic actions of neoagarotetraose ( Liu et al, 2020 ). We put forth that pigeon pea derived metabolites in the diet do merit evaluation for actions in the human gut.…”

Fructooligosaccharide supplementation alleviated the pathological immune response and prevented the impairment of intestinal barrier in DSS-induced acute colitis mice

“…Many studies suggested the signatures of micriobial composition and metabolites might be used to predict the action mechanisms of prebiotic or probiotic. 24,[35][36][37][38] The presenting results suggested that FOS supplementation might prevent the DSS-induced dysbiosis of gut microbiota, by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum and Bifidobacterium) and decreasing the abundance of potentially harmful bacteria (e.g., Mucispirillum), but the mechanisms need to be further explored.…”