Molecular and next-generation sequencing analysis of tick-borne pathogens of Rhipicephalus ticks (Acari: Ixodidae) in cattle and dogs

Intirach, Jitrawadee; Lv, Xin; Sutthanont, Nataya; Cai, Benchi; Champakaew, Danita; Chen, Tao; Han, Qian; Lv, Zhiyue

doi:10.1016/j.actatropica.2024.107138

Cited by 3 publications

(3 citation statements)

References 61 publications

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“…In this study, T. annulata was only detected in H. asiaticum , and T. orientalis was only identified in R. microplus ticks. However, the vector roles of both H. asiaticum and R. microplus are still to be determined, despite the frequent reports of Theileria in them [ 27 – 31 ]. For T. orientalis , although its DNA has been detected in eggs of R. microplus tick [ 32 ], suggesting the existence of transovarial transmission, a study performed by Ghafar et al [ 33 ] indicated that R. microplus fails to transmit T. orientalis .…”

Section: Discussionmentioning

confidence: 99%

Investigation of Babesia spp. and Theileria spp. in ticks from Western China and identification of a novel genotype of Babesia caballi

Zhang,

Gao

et al. 2024

BMC Vet Res

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Babesia spp. and Theileria spp. are tick-borne protozoan parasites with veterinary importance. In China, epidemiological and genetic investigations on many Babesia and Theileria species were still absent in many areas and many tick species. From Aug 2021 to May 2023, 645 ticks were collected from the body surface of domestic animals (camels, goats, sheep, and cattle) using tweezers in seven counties in three provinces including Xinjiang (Qitai, Mulei, Hutubi, and Shihezi counties), Chongqing (Youyang and Yunyang counties), and Qinghai (Huangzhong county). Three tick species were morphologically and molecularly identified (334 Hyalomma asiaticum from Xinjiang, 245 Rhipicephalus microplus from Chongqing, and 66 Haemaphysalis qinghaiensis from Qinghai). A total of three Babesia species and two Theileria species were detected targeting the 18S gene. The COI and cytb sequences were also recovered from Babesia strains for further identification. In R. microplus from Chongqing, Babesia bigemina, the agent of bovine babesiosis, was detected. Notably, in H. asiaticum ticks from Xinjiang, a putative novel genotype of Babesia caballi was identified (0.90%, 3/334), whose COI and cytb genes have as low as 85.82% and 90.64–90.91% nucleotide identities to currently available sequences. It is noteworthy whether the sequence differences of its cytb contribute to the drug resistance of this variant due to the involvement of cytb in the drug resistance of Babesia. In addition, Theileria orientalis and Theileria annulata were detected in R. microplus from Chongqing (12.20%, 31/245) and H. asiaticum from Xinjiang (1.50%, 5/334), respectively. These results suggest that these protozoan parasites may be circulating in domestic animals in these areas. The pathogenicity of the novel genotype of B. caballi also warrants further investigation.

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Section: Discussionmentioning

confidence: 99%

Investigation of Babesia spp. and Theileria spp. in ticks from Western China and identification of a novel genotype of Babesia caballi

Zhang,

Gao

et al. 2024

BMC Vet Res

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“…This is not the only research using NGS on tick-borne pathogens. Intirach et al also obtained interesting results [ 77 ]. They performed tick’s pathogens screening based on PCR and NGS.…”

Section: Infectious Diseases In Animalsmentioning

confidence: 99%

“…Illumina NovaSeq sequencing showed a unique dominance of Bacteroidota in Rhipicephalus spp. [ 77 ]. This approach shed light into tick-borne illnesses.…”

Section: Infectious Diseases In Animalsmentioning

confidence: 99%

Application of Next-Generation Sequencing (NGS) Techniques for Selected Companion Animals

Domrazek,

Jurka

2024

Animals

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Next-Generation Sequencing (NGS) techniques have revolutionized veterinary medicine for cats and dogs, offering insights across various domains. In veterinary parasitology, NGS enables comprehensive profiling of parasite populations, aiding in understanding transmission dynamics and drug resistance mechanisms. In infectious diseases, NGS facilitates rapid pathogen identification, characterization of virulence factors, and tracking of outbreaks. Moreover, NGS sheds light on metabolic processes by elucidating gene expression patterns and metabolic pathways, essential for diagnosing metabolic disorders and designing tailored treatments. In autoimmune diseases, NGS helps identify genetic predispositions and molecular mechanisms underlying immune dysregulation. Veterinary oncology benefits from NGS through personalized tumor profiling, mutation analysis, and identification of therapeutic targets, fostering precision medicine approaches. Additionally, NGS plays a pivotal role in veterinary genetics, unraveling the genetic basis of inherited diseases and facilitating breeding programs for healthier animals. Physiological investigations leverage NGS to explore complex biological systems, unraveling gene–environment interactions and molecular pathways governing health and disease. Application of NGS in treatment planning enhances precision and efficacy by enabling personalized therapeutic strategies tailored to individual animals and their diseases, ultimately advancing veterinary care for companion animals.

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Update on tick-borne pathogens detection methods within ticks

Krupa,

Dziedziech,

Paul

et al. 2024

Current Research in Parasitology & Vector-Borne Diseases

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Molecular and next-generation sequencing analysis of tick-borne pathogens of Rhipicephalus ticks (Acari: Ixodidae) in cattle and dogs

Cited by 3 publications

References 61 publications

Investigation of Babesia spp. and Theileria spp. in ticks from Western China and identification of a novel genotype of Babesia caballi

Investigation of Babesia spp. and Theileria spp. in ticks from Western China and identification of a novel genotype of Babesia caballi

Application of Next-Generation Sequencing (NGS) Techniques for Selected Companion Animals

Update on tick-borne pathogens detection methods within ticks

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