2009
DOI: 10.3103/s1068367409050085
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Molecular approaches in identifying leaf rust resistance genes in Russian wheat varieties

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Cited by 22 publications
(10 citation statements)
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“…DNA quality was verified by electrophoresis in 0.8% agarose gel, stained with ethidium bromide, visualized under UV light and compared with ladder Lambda DNA/HindIII (Fermentas, Vilnius, Lithuania). The genes Lr10, Lr24, Lr26, Lr28, Lr34, and Lr37 were tested by PCR with published primers marking these genes according to de Froidmont (1998), Helguera et al (2003), Cherukuri et al (2005), Gupta et al (2006), Lagudah et al (2006), Gultyaeva et al (2009). The reactions were carried out in the Veriti thermal cycler (Applied Biosystems, Foster City, USA).…”
Section: Methodsmentioning
confidence: 99%
“…DNA quality was verified by electrophoresis in 0.8% agarose gel, stained with ethidium bromide, visualized under UV light and compared with ladder Lambda DNA/HindIII (Fermentas, Vilnius, Lithuania). The genes Lr10, Lr24, Lr26, Lr28, Lr34, and Lr37 were tested by PCR with published primers marking these genes according to de Froidmont (1998), Helguera et al (2003), Cherukuri et al (2005), Gupta et al (2006), Lagudah et al (2006), Gultyaeva et al (2009). The reactions were carried out in the Veriti thermal cycler (Applied Biosystems, Foster City, USA).…”
Section: Methodsmentioning
confidence: 99%
“…DNA quality was verified by electrophoresis in 0.8% agarose gel, stained with ethidium bromide, visualized under UV light and compared with ladder Lambda DNA/ HindIII (Fermentas). The genes Lr10, Lr34, Lr26 and Lr37 were identified with the use of PCR with published primers marking these genes (de Froidmont et al 1998;Helguera et al 2003;Lagudah et al 2006;Gultyaeva et al 2009). Genes Lr26 and Lr37 were detected using multiplex PCR in one reaction.…”
Section: Methodsmentioning
confidence: 99%
“…Исследования включали три этапа: первый этап -для подтверждения наличия чужеродного материала у исследуемых почти изогенных линий Саратовская 68*4//TcLr28 (С68 Lr28) была проведена идентификация Lr-генов с использованием молекулярного маркера Lr28 (SCS421) [8]. ПЦР-анализ был выполнен по ранее опубликованной методике [9]. Продукты амплификации были выявлены в ультрафиолетовом свете на 1,5 % агарозном геле, содержащем этидиум бромид.…”
Section: материалы и методы исследованияunclassified