Molecular approaches to enhance astaxanthin biosynthesis; future outlook: engineering of transcription factors in
            <i>Haematococcus pluvialis</i>

Kayani, Sadaf‐Ilyas; Rahman, Saeed-ur; Shen, Qian; Cui, Yi; Liu, Wei; Hu, Xinjuan; Zhu, Feifei; Huo, Shuhao

doi:10.1080/07388551.2023.2208284

Cited by 5 publications

(3 citation statements)

References 136 publications

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“…Although the TFE has been widely applied in genetic engineering modification of yeast and microalgae, various TFs regulating microbial astaxanthin synthesis have been continuously being discovered; the genetic modification of astaxanthin biosynthesis based on TFE technology has not been reported ( Grama et al, 2022 ). The potential of TFE to increase astaxanthin productivity in H. pluvialis has been recognized and proposed ( Kayani et al, 2023 ). In this study, a biochemical and genetic strategy based on hormone treatment and TFE is applied in the astaxanthin-producing yeast P. rhodozyma for the first time and simultaneously increased its cell growth and astaxanthin synthesis, confirming that our strategy is efficient and lay the foundation for microbial astaxanthin production.…”

Section: Discussionmentioning

confidence: 99%

The enhancement of astaxanthin production in Phaffia rhodozyma through a synergistic melatonin treatment and zinc finger transcription factor gene overexpression

Jia,

Chen,

et al. 2024

Front. Microbiol.

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Astaxanthin has multiple physiological functions and is applied widely. The yeast Phaffia rhodozyma is an ideal source of microbial astaxanthin. However, the stress conditions beneficial for astaxanthin synthesis often inhibit cell growth, leading to low productivity of astaxanthin in this yeast. In this study, 1 mg/L melatonin (MT) could increase the biomass, astaxanthin content, and yield in P. rhodozyma by 21.9, 93.9, and 139.1%, reaching 6.9 g/L, 0.3 mg/g DCW, and 2.2 mg/L, respectively. An RNA-seq-based transcriptomic analysis showed that MT could disturb the transcriptomic profile of P. rhodozyma cell. Furthermore, differentially expressed gene (DEG) analysis show that the genes induced or inhibited significantly by MT were mainly involved in astaxanthin synthesis, metabolite metabolism, substrate transportation, anti-stress, signal transduction, and transcription factor. A mechanism of MT regulating astaxanthin synthesis was proposed in this study. The mechanism is that MT entering the cell interacts with components of various signaling pathways or directly regulates their transcription levels. The altered signals are then transmitted to the transcription factors, which can regulate the expressions of a series of downstream genes as the DEGs. A zinc finger transcription factor gene (ZFTF), one of the most upregulated DEGs, induced by MT was selected to be overexpressed in P. rhodozyma. It was found that the biomass and astaxanthin synthesis of the transformant were further increased compared with those in MT-treatment condition. Combining MT-treatment and ZFTF overexpression in P. rhodozyma, the biomass, astaxanthin content, and yield were 8.6 g/L, 0.6 mg/g DCW, and 4.8 mg/L and increased by 52.1, 233.3, and 399.7% than those in the WT strain under MT-free condition. In this study, the synthesis and regulation theory of astaxanthin is deepened, and an efficient dual strategy for industrial production of microbial astaxanthin is proposed.

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Section: Discussionmentioning

confidence: 99%

The enhancement of astaxanthin production in Phaffia rhodozyma through a synergistic melatonin treatment and zinc finger transcription factor gene overexpression

Jia,

Chen,

et al. 2024

Front. Microbiol.

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“…However, the regulatory mechanism of carotenoid metabolism in algae is still seriously lacking and even in some carotenoidrich algae such as the β-carotene-accumulating D. salina 6 and the astaxanthin-accumulating H. pluvialis. 134 The current understanding of the functions of regulators including transcription factors and regulatory proteins in algae is still limited, mainly because the genetic background of most algae, especially eukaryotic algae, is not well understood. For the genetic manipulation of algae at present, very few algal species can perform genetic manipulation efficiently, except for the model species C. reinhardtii.…”

Section: Future Challenges and Prospectsmentioning

confidence: 99%

“…salina and the astaxanthin-accumulating H. pluvialis . The current understanding of the functions of regulators including transcription factors and regulatory proteins in algae is still limited, mainly because the genetic background of most algae, especially eukaryotic algae, is not well understood.…”

Section: Future Challenges and Prospectsmentioning

confidence: 99%

Involvement of Transcription Factors and Regulatory Proteins in the Regulation of Carotenoid Accumulation in Plants and Algae

Liang,

2023

J. Agric. Food Chem.

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Efficient astaxanthin production: Advanced strategies to improve microbial fermentation

Zhou,

Yang,

Jiang

et al. 2024

Biofuels Bioprod Bioref

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Astaxanthin is a very valuable chemical with strong antioxidant effects, including anti‐cancer, anti‐inflammatory, eye protection, and other properties. The rapid development of synthetic biology has facilitated microbial astaxanthin production, offering environmental benefits, mild reaction conditions, and alignment with consumer demand for natural compounds. Accordingly, this review introduces the latest progress in the production of astaxanthin using different microorganisms including native microbes like Haematococcus pluvialis and Xanthophyllomyces dendrorhous, as well as engineered microbes like Yarrowia lipolytica and Escherichia coli. Methods for improving astaxanthin production through fermentation process regulation and metabolic engineering are reviewed and directions for future work are proposed.

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Molecular approaches to enhance astaxanthin biosynthesis; future outlook: engineering of transcription factors in Haematococcus pluvialis

Cited by 5 publications

References 136 publications

The enhancement of astaxanthin production in Phaffia rhodozyma through a synergistic melatonin treatment and zinc finger transcription factor gene overexpression

The enhancement of astaxanthin production in Phaffia rhodozyma through a synergistic melatonin treatment and zinc finger transcription factor gene overexpression

Involvement of Transcription Factors and Regulatory Proteins in the Regulation of Carotenoid Accumulation in Plants and Algae

Efficient astaxanthin production: Advanced strategies to improve microbial fermentation

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