Molecular assay optimized by Taguchi experimental design method for venous thromboembolism investigation

Souza, Helder Jose Celani de; Moysés, Cinthia Bachir; Pontes, Fabrício José; Duarte, Roberto N.; Silva, Carlos Eduardo Sanches da; Alberto, Fernando; Ferreira, Ubirajara Rocha; Silva, Messias Borges

doi:10.1016/j.mcp.2011.08.001

Cited by 16 publications

(12 citation statements)

References 11 publications

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“…As an alternative approach to reduce time and effort, the Taguchi method [21], [22], [23], was first applied to molecular biological methodologies for the optimization of traditional end point PCR in 1994 [24]. The Taguchi method has also been used for DNA amplification fingerprinting [25], end-point multiplex PCR [26], [27], [28], real-time quantitative PCR using SYBR Green chemistry [29] and fluorescence resonance energy transfer (FRET) probes [30]. Although this method has been effective for several molecular assays, it has yet to be demonstrated for AS-PCR [31].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Specificity of TPMT*2 Genotyping Using Unidirectional Wild-Type and Mutant Allele-Specific Scorpion Primers in a Single Tube

et al. 2014

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Genotyping of thiopurine S-methyltransferase (TPMT) is recommended for predicting the adverse drug response of thiopurines. In the current study, a novel version of allele-specific PCR (AS-PCR), termed competitive real-time fluorescent AS-PCR (CRAS-PCR) was developed to analyze the TPMT*2 genotype in ethnic Chinese. This technique simultaneously uses wild-type and mutant allele-specific scorpion primers in a single reaction. To determine the optimal conditions for both traditional AS-PCR and CRAS-PCR, we used the Taguchi method, an engineering optimization process that balances the concentrations of all components using an orthogonal array rather than a factorial array. Instead of running up to 264 experiments with the conventional factorial method, the Taguchi method achieved the same optimization using only 16 experiments. The optimized CRAS-PCR system completely avoided non-specific amplification occurring in traditional AS-PCR and could be performed at much more relaxed reaction conditions at 1% sensitivity, similar to traditional AS-PCR. TPMT*2 genotyping of 240 clinical samples was consistent with published data. In conclusion, CRAS-PCR is a novel and robust genotyping method, and the Taguchi method is an effective tool for the optimization of molecular analysis techniques.

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Section: Introductionmentioning

confidence: 99%

Enhanced Specificity of TPMT*2 Genotyping Using Unidirectional Wild-Type and Mutant Allele-Specific Scorpion Primers in a Single Tube

et al. 2014

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“…The most appropriate statistical methods for investigating influential variables in processes is the design of experiments (DOE). Design of Experiments can be used in process development and process troubleshooting, to improve your performance or to achieve a robust process (Souza et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

Application of Principal Component Analysis and Response Surface Methodology in the Process of Steel Wire Tempering

Pimenta¹,

Silva²,

Campos³

et al. 2019

IJSP

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The purpose of this work was the creation of a statistical modeling capable of replacing the process used to set up of the ovens of the quenching and tempering who is traditionally accomplished through adjustments made based on the results of mechanical properties tested in laboratory and required in customer specifications. This study seeks to understand the influence of input variables (factors) on the tensile strength limit, in SAE 9254 draw steel wires, with diameters 2.00 mm and 6.50 mm, used in the manufacture of valve springs and clutch springs for automobiles. The process input variables were investigated: steel wire diameter, processing speed, temper temperature and liquid polymer concentration (which is the tempering medium). Methodologies were used where design of experiments, multiple regression and quadratic regression, principal components analysis (multivariate statistical) and response surface methodology. Results revealed which variables are significant in the process. Also models obtained were validated using appropriate statistical methods. If this study is used, it can provide the automation of this process. It’s important to point out that it could impact the increase in productivity and quality of product.

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“…The Taguchi Method therefore has been utilized for improving the industry and optimizing parameter design. For instance, Su et al [10] effectively enhanced optical whiteness with Taguchi's Dynamic Approach, Hong [11] identified the key factors in the market segment with the Taguchi Method, and Souza et al [12] applied the Taguchi Method to improving the quality of hemodialysis. By computing the target function and transforming it into a Signal-to-Noise ratio, the Signal-to-Noise ratio of the quality characteristics is divided into the Larger the Better, Nominal the Better, and the Smaller the Better in the definition of Taguchi Method.…”

Section: Taguchi Methodsmentioning

confidence: 99%

“…The total variability of the S/N ratio was measured by the sum of the squared deviations from the total mean S/N ratio. Su et al [10], Chen and Ou [24], and Souza et al [12] applied P test values to proceed with the decision-making process. The P value was calculated for each design parameter.…”

Section: Anovamentioning

confidence: 99%

Integrating the Taguchi Method and Response Surface Methodology for Process Parameter Optimization of the Injection Molding

Jou¹,

Lin²,

Lee³

et al. 2014

Appl. Math. Inf. Sci.

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Abstract:The outer coating of an optical fiber cable protects the optical fiber, and the contraction rate of the outer coating can affect the communication capacity and distance, so the contraction rate is often regarded as the key factor in defining the quality of an optical fiber cable. For this reason, using the example of the circular single-pin optical fiber cable manufacturing process of the largest optical communication manufacturer in the world, the Taguchi Method (TM) is used to screen the variables that have significant effects on the contraction rate of the outer coating. Furthermore, the optimization engineering of Response Surface Methodology (RSM) is utilized for the empirical research to acquire a prediction model that can be used to optimize the optical fiber outer coating injection molding process. The research results show that the contraction rate predicted by the integration of the Taguchi Method and RSM is 2.28%. The empirical results reveal that the contraction rate of the outer coating indeed decreased from the original 3.83% down to 2.16%, indicating that the integration of the Taguchi Method and RSM could effectively improve the quality.

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Molecular assay optimized by Taguchi experimental design method for venous thromboembolism investigation

Cited by 16 publications

References 11 publications

Enhanced Specificity of TPMT*2 Genotyping Using Unidirectional Wild-Type and Mutant Allele-Specific Scorpion Primers in a Single Tube

Enhanced Specificity of TPMT*2 Genotyping Using Unidirectional Wild-Type and Mutant Allele-Specific Scorpion Primers in a Single Tube

Application of Principal Component Analysis and Response Surface Methodology in the Process of Steel Wire Tempering

Integrating the Taguchi Method and Response Surface Methodology for Process Parameter Optimization of the Injection Molding

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