Molecular barcoding reveals unexpected diversity in eastern North American stitchworts (Caryophyllaceae)

Abstract

Generic delimitation in Caryophyllaceae has been challenging and has been informed most recently by use of molecular phylogenetic data. In this study, analysis of 29 samples from the small segregate Mononeuria using nuclear ITS and plastid rps16 data revealed it to be polyphyletic. The type species, (M. patula) and two other species (M. muscorum and M. paludicola) were shown to belong to the Sabulina clade. The remaining species formed a clade that also included the previously monotypic Geocarpon and was sister to a heterogeneous group that included the Hawaiian Schiedea and three other monotypic genera, Honckenya, Wilhelmsia and Triplateia. Although several nomenclatural options are available, we propose to place the species from this clade into a single genus, Geocarpon, which basically follows the most recent treatment after exclusion of Sabulina spp., but with the necessary new genus placements. New combinations are proposed: Sabulina muscorum; Sabulina paludicola; Geocarpon carolinianum; Geocarpon cumberlandensis; Geocarpon glabrum; Geocarpon groendlandicum; Geocarpon nuttallii and Geocarpon uniflorum. Analysis of the sequence data revealed remarkable variability among populations of Sabulina patula (formerly Mononeuria patula), Sabulina paludicola (formerly Mononeuria paludicola) and Geocarpon groenlandicum (formerly Mononeuria groenlandica), suggesting that cryptic species may be present. The data also suggested that broader sampling of Sabulina and Geocarpon could lead to increased understanding of the timing and origins of occupation of calcareous glades and rock outcrop habitats in eastern North America.

Resolving evolutionary relationships in the groundsels: phylogenomics, divergence time estimates, and biogeography ofPackera(Asteraceae: Senecioneae)

“…However, our results suggest P. schweinitziana originated 8.4MY (min: 8.1MY, max: 8.7MY; treePL) or 7.5MY (min: 3.8MY, max: 14.9MY; RelTime) (Supplemental Materials), almost three to four times as old as Barkley's prediction. Even so, our estimated dates are consistent with research into other high-elevation rock outcrop species, similar to P. schweinitziana, that predict dates later in the Miocene (Meseguer et al, 2015;Quinlan et al, 2020;Stubbs et al, 2020;Schilling et al, 2022).…”

Utilizing herbarium specimens to assist with the listing of rare plants

“…Having a clear understanding of the nomenclature and the changes it has undergone in the past is also key to the legal protection of any rare species (Mace, 2004), including plants (Standley, 1992;Schatz, 2002). For example, if an SSA was to be developed for Minuartia patula, which has undergone multiple changes of nomenclature and delimitation (Kartesz, 1994;Kartesz, 1999;Dillenberger and Kadereit, 2014;Schilling et al, 2022), under which sense [or taxon concept (Franz et al, 2008;Sigovini et al, 2016)] would it be recognized? In cases such as these, consulting herbarium specimens, taxonomic databases and literature, or even taxonomic experts to determine the taxonomic unit of conservation is prudent.…”