Molecular Basis of Interactions between the Antibiotic Nitrofurantoin and Human Serum Albumin: A Mechanism for the Rapid Drug Blood Transportation

Calderaro, Antonella; Maugeri, Alessandro; Magazù, Salvatore; Laganà, Giuseppina; Navarra, Michele; Barreca, Davide

doi:10.3390/ijms22168740

Cited by 12 publications

(7 citation statements)

References 47 publications

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“…Therefore, to ensure the accuracy and reliability of chromosome preparation, it is generally recommended to perform cell culture and chromosome preparation as soon as possible after blood sample collection [20]. However, in clinical practice, blood samples may need to be stored for 24 hours or longer, or even transported to external laboratories, which can take several days [16,21]. To ensure an adequate number and vitality of cells for chromosome preparation and analysis, considering a higher injection blood volume is a viable solution.…”

Section: Discussionmentioning

confidence: 99%

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Wei

2024

Am J Transl Res

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Objective: This study aims to investigate the impact of refrigeration time and blood volume on the success rate of peripheral blood chromosomal analysis using response surface methodology (RSM). Methods: Peripheral blood samples from 30 volunteers were subjected to chromosomal analysis under different refrigeration duration periods (≤7 days, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 14 days) along with different blood volumes (0.2 mL, 0.3 mL, 0.4 mL, 0.5 mL, 0.6 mL, 0.7 mL, and 0.8 mL). The effects of refrigeration time and blood volume on the success rate of peripheral blood chromosomal analysis were determined using the Chi-square test for trend, followed with Spearman's rank correlation coefficient, and RSM analysis to identify the optimal combination of refrigeration time and blood volume. Results: The refrigeration time within 10 days had a minor impact on the success rate, while refrigeration time more than 11 days significantly decreased the success rate. An increase in blood volume slightly improved the success rate. The success rate showed both linear and nonlinear changes with refrigeration time, while the effect of blood volume was primarily linear. The highest success rate was observed at a refrigeration time of ≤7 days and a blood volume of 0.8 mL. The interaction between refrigeration time and blood volume had a significant impact on the success rate. Conclusion: It is recommended to keep the refrigeration time of blood samples within 7 days and control the blood volume at 0.8 mL to maximize the success rate of chromosomal analysis.

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Section: Discussionmentioning

confidence: 99%

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Wei

2024

Am J Transl Res

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“…where k 1 and k 2 are the retention factors of the first-and second-eluting enantiomer, respectively. The k values were used to calculate the albumin-bound percentage (b%) using Equation ( 7) by zonal elution chromatography [12]:…”

Section: Hplcmentioning

confidence: 99%

“…Since at least 90% of drugs bind to plasma proteins, a high-throughput in vivo transport protein binding result is particularly important, as HSA binding highly affects the pharmacokinetics and bioavailability of a drug [9,10]. HSA is a globular protein, and its heart-shaped structure is composed of three main domains (I-III), each containing two subdomains (A and B), characterized by the presence of α-helices in the structure, with multiple ligand-binding sites localized in each of these subdomains [11,12]. According to Sudlow's classification, there are two main binding sites for drug ligands of HSA, namely, Sudlow's site I, positioned in subdomain IIA, and site II, positioned in subdomain IIIA.…”

Section: Introductionmentioning

confidence: 99%

Enantioselective Human Serum Albumin Binding of Apremilast: Liquid Chromatographic, Fluorescence and Molecular Docking Study

Dombi

Horváth

Fiser

et al. 2023

IJMS

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The interaction between human serum albumin (HSA) and apremilast (APR), a novel antipsoriatic drug, was characterized by multimodal analytical techniques including high-performance liquid chromatography (HPLC), fluorescence spectroscopy and molecular docking for the first time. Using an HSA chiral stationary phase, the APR enantiomers were well separated, indicating enantioselective binding between the protein and the analytes. The influence of chromatographic parameters—type and concentration of the organic modifier, buffer type, pH, ionic strength of the mobile phase, flow rate and column temperature—on the chromatographic responses (retention factor and selectivity) was analyzed in detail. The results revealed that the eutomer S-APR bound to the protein to a greater extent than the antipode. The classical van ’t Hoff method was applied for thermodynamic analysis, which indicated that the enantioseparation was enthalpy-controlled. The stability constants of the protein–enantiomer complexes, determined by fluorescence spectroscopy, were in accordance with the elution order observed in HPLC (KR-APR-HSA = 6.45 × 103 M−1, KS-APR-HSA = 1.04 × 104 M−1), showing that, indeed, the later-eluting S-APR displayed a stronger binding with HSA. Molecular docking was applied to study and analyze the interactions between HSA and the APR enantiomers at the atomic level. It was revealed that the most favored APR binding occurred at the border between domains I and II of HSA, and secondary interactions were responsible for the different binding strengths of the enantiomers.

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“…In the past, several studies have been published about the interaction between different molecules and proteins of the blood [1][2][3]. There is a growing interest in the formation of the bimolecular complex between the human serum albumin (HSA) and different exogenous and endogenous compounds.…”

Section: Introductionmentioning

confidence: 99%

Anti-Aggregative and Protective Effects of Vicenin-2 on Heat and Oxidative Stress-Induced Damage on Protein Structures

Patanè,

Lombardo,

Putaggio

et al. 2023

IJMS

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Vicenin-2, a flavonoid categorized as a flavones subclass, exhibits a distinctive and uncommon C-glycosidic linkage. Emerging evidence challenges the notion that deglycosylation is not a prerequisite for the absorption of C-glycosyl flavonoid in the small intestine. Capitalizing on this experimental insight and considering its biological attributes, we conducted different assays to test the anti-aggregative and antioxidant capabilities of vicenin-2 on human serum albumin under stressful conditions. Within the concentration range of 0.1–25.0 μM, vicenin-2 effectively thwarted the heat-induced HSA fibrillation and aggregation of HSA. Furthermore, in this study, we have observed that vicenin-2 demonstrated protective effects against superoxide anion and hydroxyl radicals, but it did not provide defense against active chlorine. To elucidate the underlying mechanisms, behind this biological activity, various spectroscopy techniques were employed. UV-visible spectroscopy revealed an interaction between HSA and vicenin-2. This interaction involves the cinnamoyl system found in vicenin-2, with a peak of absorbance observed at around 338 nm. Further evidence of the interaction comes from circular dichroism spectrum, which shows that the formation of bimolecular complex causes a reduction in α-helix structures. Fluorescence and displacement investigations indicated modifications near Trp214, identifying Sudlow’s site I, similarly to the primary binding site. Molecular modeling revealed that vicenin-2, in nonplanar conformation, generated hydrophobic interactions, Pi-pi stacking, and hydrogen bonds inside Sudlow’s site I. These findings expand our understanding of how flavonoids bind to HSA, demonstrating the potential of the complex to counteract fibrillation and oxidative stress.

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Molecular Basis of Interactions between the Antibiotic Nitrofurantoin and Human Serum Albumin: A Mechanism for the Rapid Drug Blood Transportation

Cited by 12 publications

References 47 publications

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Enantioselective Human Serum Albumin Binding of Apremilast: Liquid Chromatographic, Fluorescence and Molecular Docking Study

Anti-Aggregative and Protective Effects of Vicenin-2 on Heat and Oxidative Stress-Induced Damage on Protein Structures

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