Molecular beacon based real-time PCR assay for simultaneous detection of Listeria monocytogenes and Salmonella spp. in dairy products

Singh, Jasbir; Batish, V. K.; Grover, Sunita

doi:10.1007/s13594-011-0007-8

Cited by 7 publications

(8 citation statements)

References 30 publications

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“…The conventional methods for detection of these pathogens involve identification and confirmation based on culturing on selective media along with biochemical tests and immunological assays (Quigley et al, 2013). These methods are standard methods; however, they are extremely laborious, time consuming (requiring several days), and often inconclusive (Singh et al, 2011;Bopp et al, 2016;Yu et al, 2016). For these reasons, there is an increasing demand for more rapid methods of foodborne pathogen detection (Zhao et al, 2014), in order to complement or replace the traditional microbial culture procedures with more advanced, sensitive, and rapid microbial detection methods.…”

Section: Conventional Methodsmentioning

confidence: 99%

“…The amount of generated PCR product is proportional to the increase in a fluorescent signal, which is monitored during the exponential phase (Auvolat and Besse, 2016). This technique permits rapid identification and quantification of bacteria (Singh et al, 2011;Quigley et al, 2013).…”

Section: Real-time Quantitative Pcr (Qpcr)mentioning

confidence: 99%

“…over a wide range of food products including beef, seafood, fresh produce, and dairy products (Singh et al, 2011). The sensitivity of qPCR when applied to a food matrix is generally quite limited when compared with other enumeration methods.…”

Section: Real-time Quantitative Pcr (Qpcr)mentioning

confidence: 99%

“…Biochips may allow the simultaneous detection and identification of multiple microorganisms in a relatively short time, and they are being described as a powerful tool for the detection of foodborne pathogens (Chiang et al, 2012). However, the high cost associated with this approach is the main restriction limiting its application in the dairy and food industries on a routine basis (Singh et al, 2011).…”

Section: Dna Microarraymentioning

confidence: 99%

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Foodborne bacteria in dairy products: Detection by molecular techniques

Cancino‐Padilla

Fellenberg

Franco

et al. 2017

Ciencia e investigación agraria

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Staphylococcus aureus, Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 are the most frequent potential pathogens associated with milk or dairy products in industrialized countries and are therefore the main microbiological hazards linked to raw milk and raw cheese. This review summarizes the scientific information about outbreaks related to foodborne pathogens in dairy products and highlights the increasing application of molecular approaches to detect and identify the bacteria responsible for these outbreaks. Molecular techniques have facilitated the rapid detection and identification of foodborne pathogens, which has been crucial for current surveillance and outbreak control.

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Section: Conventional Methodsmentioning

confidence: 99%

Section: Real-time Quantitative Pcr (Qpcr)mentioning

confidence: 99%

Section: Real-time Quantitative Pcr (Qpcr)mentioning

confidence: 99%

Section: Dna Microarraymentioning

confidence: 99%

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Foodborne bacteria in dairy products: Detection by molecular techniques

Cancino‐Padilla

Fellenberg

Franco

et al. 2017

Ciencia e investigación agraria

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“…[16][17][18][19][20][21] However, the traditional molecular beacon requires modified fluorophore and quencher groups at both ends of the oligonucleotide, which leads to the long preparation time and high detection cost. 22 Hoechst 33258 is a weakly fluorescent unsymmetrical cyanine acid dye.…”

Section: Original Papersmentioning

confidence: 99%

Highly Sensitive Fluorescence Quantitative Detection of Mercury in Soil Based on Non-labeled Molecular Beacon and Fluorescent Dye Hoechst 33258

et al. 2017

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Mercury (Hg) is a highly toxic substance; 1,2 it is very difficult to degrade, and easy to accumulate in crops and soil. 3,4 Mercury is introduced into the human body through the food chain, the accumulation of mercury in the body can lead to chronic poisoning, which is a serious threat to human survival and health. 5,6 Since mercury is one of the essential detection items in environmental monitoring, so the quantitative detection of mercury has very important significance.The quantitative detection methods used for mercury include atomic emission spectroscopy, 7 atomic absorption spectroscopy, 8 atomic fluorescence spectroscopy, 9 the UV-visible absorption spectroscopy, 10 inductively coupled plasma mass spectrometry, 11 cold vapor atomic fluorescence spectroscopy and gas chromatography-pyrolysis-atomic fluorescence spectroscopy, 12 liquid chromatography-cold vapor atomic absorption spectrometry and molecular fluorescence spectroscopy. [13][14][15] Among these analysis methods, atomic absorption spectroscopy is the most common, but this method needs a higher requirement for sample preparation and a long detection time. Atomic emission spectroscopy has good selectivity, low detection limit, small matrix effects and high precision, but it requires a highly delicate and very expensive instrument. Atomic fluorescence method has many advantages, such as simple spectral lines, high sensitivity, less interference and a low detection limit. However, the health of operators might be threatened because of mercury vapor generated during operation. UV-visible absorption spectroscopy has a high detection sensitivity and simple experimental equipment, but the method not only requires a long reaction time, but also the specificity of the chromogenic agent is poor. Liquid chromatography-cold vapor atomic absorption spectrometry has a high detection sensitivity and good selectivity. However, the application of this method is limited in most laboratories because it requires expensive equipment. Molecular fluorescence spectroscopy has good prospects because of it's simple operation, fast detection speed, high sensitivity, good selectivity, good reproducibility and low detection limit.

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Molecular methods for the detection of waterborne pathogens

Gilbride¹

2021

Waterborne Pathogens

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Molecular beacon based real-time PCR assay for simultaneous detection of Listeria monocytogenes and Salmonella spp. in dairy products

Abstract: International audienc

Cited by 7 publications

References 30 publications

Foodborne bacteria in dairy products: Detection by molecular techniques

Foodborne bacteria in dairy products: Detection by molecular techniques

Highly Sensitive Fluorescence Quantitative Detection of Mercury in Soil Based on Non-labeled Molecular Beacon and Fluorescent Dye Hoechst 33258

Molecular methods for the detection of waterborne pathogens

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