Molecular characterisation and antimicrobial activity of bacteria associated with submerged lactic acid cassava fermentation

Anyogu, Amarachukwu; Awamaria, Brigitte; Sutherland, Jane P.; Ouoba, L.I.I.

doi:10.1016/j.foodcont.2013.11.005

Cited by 38 publications

(33 citation statements)

References 30 publications

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“…Bacteriocins of LAB are active against Grampositive bacteria, such as LAB (García-Ruiz et al, 2013) and S. aureus (Anyogu et al, 2014;Omar et al, 2008;Sebastià et al, 2011). However, plantaricins produced by L. plantarum strains had broad spectra of inhibition activity against Gram-negative bacteria including E. coli and Salmonella enterica (Anyogu et al, 2014;Omar et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…However, plantaricins produced by L. plantarum strains had broad spectra of inhibition activity against Gram-negative bacteria including E. coli and Salmonella enterica (Anyogu et al, 2014;Omar et al, 2008). Strain L. sakei C2 producing a bacteriocin strongly inhibited S. aureus and E. coli (Gao et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Recent research revealed that LAB can produce antibacterial substances including organic acids, hydrogen peroxide, diacetyl, inhibitory enzymes and bacteriocins (Herna´ndez et al, 2005) to inhibit the growth of a wide range of intestinal pathogens (García-Ruiz et al, 2013). Bacteriocins were defined as antimicrobial peptides or proteins have been observed in many genera of bacteria, including many strains of LAB, which are directed mainly to inhibit the growth of related species (Anyogu et al, 2014). Lactobacillus rhamnosus has been reported to interact with intestinal epithelium and prevent the internalization of enterohemorrhagic E. coli (Moorthy et al, 2007).…”

Section: Escherichia Coli Andmentioning

confidence: 99%

“…Bacterial cells were removed by centrifugation (17,000 g, 10 min, 4°C) and the resulting solution were subjected to filtration with 0.22 µm filter, then, the diameters of inhibition zones were measured using the agar diffusion assay method (Anyogu et al, 2014). Overnight test cultures of E. coli and S. aureus were diluted in saline solution into 10 8 CFU/ml and 100 µl of dilution were inoculated in nutrient agar medium.…”

Section: Screening Antibacterial Activity Of Labmentioning

confidence: 99%

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Antibacterial activity Lactobacillus plantarum isolated from fermented vegetables and investigation of the plantaricin genes

Chang¹,

Hong²,

Chen³

et al. 2016

Afr. J. Microbiol. Res.

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Escherichia coli and Staphylococcus aureus are two food pathogens that cause severe food poisonings. Another problem found on a global level is the continuous increase of antimicrobial resistance in bacteria isolated from food. This study aimed to evaluate the antibacterial activity of Lactobacillus plantarum against pathogenic bacteria including E. coli and S. aureus and to study if L. plantarum with antibacterial activity contained the most plantaricin genes or not. A total of 50 lactic acid bacteria isolates (LAB) were evaluated for antibacterial activity and identified plantaricin genes by polymerase chain reaction (PCR) methods. Seven LAB isolates with antibacterial activity against S. aureus and E. coli were identified as Lactobacillus based on morphological physiological and biochemical properties. Using species-specific PCR and 16S rRNA gene sequencing, B0039 was identified as Lactobacillus paracasei, other isolates were identified as L. plantarum. 3 strains tested positive for all the genes in the plnABCD operon. The plnEFI operon was detected in four strains. Genes encoding for the two-peptide plnJ/K were detected only in 2 strains. Finally, the plnG/V was also found in 3 strains of L. plantarm. The plantarum gene sequences of B0055 were 97 to 100% similarity with the L. plantarum WCFS1. The findings suggest that LAB with bacteriocin genes can be used as an alternative mechanism to control drug resistant foodborne pathogens.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Escherichia Coli Andmentioning

confidence: 99%

Section: Screening Antibacterial Activity Of Labmentioning

confidence: 99%

See 2 more Smart Citations

Antibacterial activity Lactobacillus plantarum isolated from fermented vegetables and investigation of the plantaricin genes

Chang¹,

Hong²,

Chen³

et al. 2016

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…Bioactive peptides play an important role in metabolic regulation and modulation, including antioxidant, antihypertensive, antimicrobial and immunomodulatory activities (Anyogu et al, 2014;Kim & Wijesekara, 2010;Korhonen & Pihlanto, 2006;Zheng et al, 2014). Immunomodulatory peptides can enhance immune cell functions such as lymphocyte proliferation, natural killer (NK) cell activity, antibody synthesis, and cytokine regulation (Singh et al, 2014).…”

Section: Introductionmentioning

confidence: 99%