Molecular characteristics and temperature tolerance function of the transient receptor potential in the native Bemisia tabaci AsiaII3 cryptic species

Ji, Shun-Xia; Shen, Xiantao; Liang, Lin; Wang, Xiaodi; Liu, Wan‐Xue; Wan, Fang‐Hao; Lu, Zengkui

doi:10.1016/s2095-3119(20)63226-9

Cited by 4 publications

(6 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ankyrin repeats are about 33 amino acids in length and have at least four contiguous copies that are involved in protein-protein interactions. Interestingly, there are eight ankyrin repeats in T. absoluta but less in other reported insects [52,54]. According to the reported genome sequence of Tribolium castaneum [54], the Painless protein of T. castaneum was found to have six ankyrin repeats by SMART analysis, and TcPain played a role in the rapid acclimation to high temperature [55]; while only two ankyrin repeats were found in the TRP study of B. tabaci, and BtTRP played a role in thermal tolerance at 35 • C [52].…”

Section: Discussionmentioning

confidence: 93%

“…Interestingly, there are eight ankyrin repeats in T. absoluta but less in other reported insects [52,54]. According to the reported genome sequence of Tribolium castaneum [54], the Painless protein of T. castaneum was found to have six ankyrin repeats by SMART analysis, and TcPain played a role in the rapid acclimation to high temperature [55]; while only two ankyrin repeats were found in the TRP study of B. tabaci, and BtTRP played a role in thermal tolerance at 35 • C [52]. It can be calculated that the number of anchors varies greatly among different species.…”

Section: Discussionmentioning

confidence: 93%

“…In the study of two cryptic species of Bemisia tabaci AsiaII3 and MEAM1 (Middle East Asia Minor 1), it was found that temperature stress could induce the expression of BtTRP mRNA, and played an important role in heat tolerance. According to this characteristic, researchers infer that BtTRP also belongs to the TRPA subfamily [51][52][53]. Therefore, the TRPA subfamily plays an important role in temperature sensing and the heat escape injury response.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Characterization of TRPA Subfamily Genes and Function in Temperature Preference in Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Wang

Lin

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

To reveal the mechanism of temperature preference in Tuta absoluta, one of the top 20 plant pests in the world, we cloned and identified TaTRPA1, TaPain, and TaPyx genes by RACE and bioinformatic analysis, and clarified their expression profiles during different development stages using real-time PCR, and revealed their function in preference temperature by RNAi. The full-length cDNA of TaPain was 3136 bp, with a 2865-bp open reading frame encoding a 259.89-kDa protein; and the partial length cDNA of TaPyx was 2326-bp, with a 2025-bp open reading frame encoding a 193.16-kDa protein. In addition, the expression of TaTRPA1 and TaPyx was significantly lower in larvae than other stages, and it was significantly higher in pupae and newly emerging males for TaPain. After feeding target double-stranded RNA (dsRNA), the preferred temperature decreased 2 °C more than the control group. In conclusion, the results firstly indicated the molecular characterization of TRPA subfamily genes and their key role in temperature perception in T. absoluta, and the study will help us to understand the temperature-sensing mechanism in the pest, and will provide some basis for study of other Lepidoptera insects’ temperature preference. Moreover, it is of great significance in enriching the research progress of “thermos TRP”.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Characterization of TRPA Subfamily Genes and Function in Temperature Preference in Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Wang

Lin

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The invasive MED and native Asia II 1 used in this study were reared separately in the laboratory, and species were confirmed every month by the mitochondrial cytochrome oxidase I gene sequence according to the primer sequences reported by Ji et al (2020) . Whiteflies were reared on healthy cotton plants, Gossypium hirsutum (L.) (Zhong No.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Chromatin Remodeling Genes Involved in Thermal Tolerance of Biologically Invasive Bemisia tabaci

Wang

Lin

et al. 2022

Front. Physiol.

Self Cite

View full text Add to dashboard Cite

As an invasive species, Bemisia tabaci Mediterranean (MED) has notable potential to adapt to a wide range of environmental temperatures, which enables it to successfully spread after invasion and occupy habitats over a wide latitude range. It has been postulated that chromatin remodeling mechanisms are related to the rapid acquisition of adaptive traits and thermal resistance in invasive species; however, relevant experimental evidence is scarce. To identify the molecular characteristics and assess the role of chromatin remodelers in thermal stress within invasive MED and native Asia II 1 of the B. tabaci species complex, we identified 13 switching defective/sucrose non-fermenting (SWI/SNF) and 10 imitation switch (ISWI) family members in the B. tabaci genome, analyzed their molecular characteristics and structures, and identified key mutation sites between MED and Asia II 1, then cloned the catalytic subunits, and revealed the difference in thermal tolerance function. The results showed that the expression levels of Bt-BRM-1 and Bt-BRM-2 were significantly higher in MED than in Asia II 1 during heat stress, and Bt-BRM-2 expression was significantly higher during cold stress. In addition, RNA interference results indicated that the two target genes had similar temperature tolerance function in the both two cryptic species. This study is the first to identify and analyze the molecular characteristics of SWI/SNF and ISWI family members and reveal their potential key roles in temperature tolerance in poikilothermic ectotherms. The results will assist in understanding the underlying temperature adaptation mechanism of invasive insects and will enrich stress adaptation research systems from an epigenetic perspective.

show abstract

“…Similarly, an organism adapts to a natural climatic condition, fluctuating between day and night (Buckley & Huey, 2016; Somero, 2010). B. tabaci MED is a high heat‐tolerant organism capable of surviving at high temperatures (Dai et al, 2018; Han et al, 2013; Ji et al, 2020; Xiao et al, 2016; Yu et al, 2012). This unique ability help whiteflies to survive in hot weather in open fields as well as closed systems, such as greenhouses, warehouses, and storage facilities.…”

Section: Introductionmentioning

confidence: 99%

Developing extreme heat acclimation in Bemisia tabaci Mediterranean (Hemiptera: Aleyrodidae)

Jhan

Lee

2022

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Bemisia tabaci Mediterranean (MED) is a highly invasive cryptic species complex found in the world's tropical, subtropical, and temperate regions. It is a severe pest of various crops and a vector of plant pathogenic viruses, particularly geminiviruses. Thermal acclimation of insects is a critical for the survival in unfavorable temperature condition. We observed that great survival rate of B. tabaci MED at the uncontrolled greenhouse which had fluctuating temperature condition (FTC) from 10°C to 60°C in spring and summer season. Our study showed that while B. tabaci MED reared under FTC for 10 weeks from April to June, its survival rate was gradually increased when heat shock was treated 50°C for 0.5 h. In contrast, the same heat shock treatment was lethal in the colony reared under constant temperature condition (CTC) at the controlled insectary. After being acclimated, the lethal temperatures LT50, LT95, and LT100 under CTC were 47.7°C, 50.1°C, and 50.3°C, whereas those under FTC were 59.8°C, 62.7°C, and 63.0°C, respectively. In addition, we observed that the transcript levels of three investigated heat shock protein (HSP) genes (hsp20, hsp70, and hsp90) were lower under FTC than under CTC. This study suggests that B. tabaci MED retains high heat acclimation ability, making it tolerant of extreme thermal conditions.

show abstract

Molecular characteristics and temperature tolerance function of the transient receptor potential in the native Bemisia tabaci AsiaII3 cryptic species

Cited by 4 publications

References 43 publications

Molecular Characterization of TRPA Subfamily Genes and Function in Temperature Preference in Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Molecular Characterization of TRPA Subfamily Genes and Function in Temperature Preference in Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Characterization of Chromatin Remodeling Genes Involved in Thermal Tolerance of Biologically Invasive Bemisia tabaci

Developing extreme heat acclimation in Bemisia tabaci Mediterranean (Hemiptera: Aleyrodidae)

Contact Info

Product

Resources

About