Molecular Characterization and Comparative Genomic Analysis of vB_PaeP_YA3, a Novel Temperate Bacteriophage of Pseudomonas aeruginosa

Yu, Xinyan; Xu, Jianping; Gu, Yu; Zhang, Ruiyang; Zhu, Yefei; Liu, Xiaoqiu

doi:10.3389/fmicb.2020.00947

Cited by 6 publications

(3 citation statements)

References 64 publications

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“…The phage infects Pseudomonas aeruginosa , an important opportunistic pathogen that can cause chronic infections leading to significant morbidity and mortality [ 20 , 21 , 22 ]. Recent studies have discovered new temperate bacteriophages infecting P. aeruginosa [ 23 , 24 ]. A GenBank database search has revealed even more unpublished bacteriophages with a lambdoid organisation of the genome.…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas Phage MD8: Genetic Mosaicism and Challenges of Taxonomic Classification of Lambdoid Bacteriophages

Evseev

Lukianova

Сыкилинда

et al. 2021

IJMS

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Pseudomonas phage MD8 is a temperate phage isolated from the freshwater lake Baikal. The organisation of the MD8 genome resembles the genomes of lambdoid bacteriophages. However, MD8 gene and protein sequences have little in common with classified representatives of lambda-like phages. Analysis of phage genomes revealed a group of other Pseudomonas phages related to phage MD8 and the genomic layout of MD8-like phages indicated extensive gene exchange involving even the most conservative proteins and leading to a high degree of genomic mosaicism. Multiple horizontal transfers and mosaicism of the genome of MD8, related phages and other λ-like phages raise questions about the principles of taxonomic classification of the representatives of this voluminous phage group. Comparison and analysis of various bioinformatic approaches applied to λ-like phage genomes demonstrated different efficiency and contradictory results in the estimation of genomic similarity and relatedness. However, we were able to make suggestions for the possible origin of the MD8 genome and the basic principles for the taxonomic classification of lambdoid phages. The group comprising 26 MD8-related phages was proposed to classify as two close genera belonging to a big family of λ-like phages.

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Section: Introductionmentioning

confidence: 99%

Pseudomonas Phage MD8: Genetic Mosaicism and Challenges of Taxonomic Classification of Lambdoid Bacteriophages

Evseev

Lukianova

Сыкилинда

et al. 2021

IJMS

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“…Multifunctional ORF 46, a transcriptional regulatory element, might functions as cI with Helix-turn-Helix_17 domain (HHphred probability 98.37%, E value 0.0000013) and Phage_Cox (HHphred probability 98.38%, E value 0.0000021). Phage_Cox might perform excision during lytic cycle of SGP-C ( Yu et al, 2020 ). A key phenomenon of Cro gene downregulation/Genes silencing guided by affinity-based binding of phage repressor proteins, thus decide fate of temperate phage genetic diversion ( Aggarwal et al, 1988 ; Waldor and Friedman, 2005 ; Hobbs and Abedon, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

SGP-C: A Broad Host Range Temperate Bacteriophage; Against Salmonella gallinarum

et al. 2022

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Salmonella gallinarum is a poultry restricted-pathogen causing fowl-typhoid disease in adult birds with mortality rates up-to 80% and exhibit resistance against commonly used antibiotics. In this current study, a temperate broad host range bacteriophage SGP-C was isolated against S. gallinarum from poultry digesta. It showed infection ability in all the 15 tested field strains of S. gallinarum. The SGP-C phage produced circular, turbid plaques with alternate rings. Its optimum activity was observed at pH 7.0 and 37–42°C, with a latent period of 45 min and burst size of 187 virions/bacterial cell. The SGP-C lysogens, SGPC-L5 and SGPC-L6 exhibited super-infection immunity against the same phage, an already reported feature of lysogens. A virulence index of 0.5 and 0.001 as MV50 of SGP-C suggests its moderate virulence. The genome of SGP-C found circular double stranded DNA of 42 Kbp with 50.04% GC content, which encodes 63 ORFs. The presence of repressor gene at ORF49, and absence of tRNA sequence in SGP-C genome indicates its lysogenic nature. Furthermore, from NGS analysis of lysogens we propose that SGP-C genome might exist either as an episome, or both as integrated and temporary episome in the host cell and warrants further studies. Phylogenetic analysis revealed its similarity with Salmonella temperate phages belonging to family Siphoviridae. The encoded proteins by SGP-C genome have not showed homology with any known toxin and virulence factor. Although plenty of lytic bacteriophages against this pathogen are already reported, to our knowledge SGP-C is the first lysogenic phage against S. gallinarum reported so far.

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“…Eight of these sequences are full-length lambda phage sequences; the remaining predicted prophages in this connected component are small sequences, partial representatives of the lambda phage genome (see Table S1 in the supplemental material). A lambda-like phage has recently been described for P. aeruginosa ( 80 ).…”

Section: Discussionmentioning

confidence: 99%

Diversity of Pseudomonas aeruginosa Temperate Phages

Johnson

Banerjee

Putonti

2022

mSphere

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Molecular Characterization and Comparative Genomic Analysis of vB_PaeP_YA3, a Novel Temperate Bacteriophage of Pseudomonas aeruginosa

Cited by 6 publications

References 64 publications

Pseudomonas Phage MD8: Genetic Mosaicism and Challenges of Taxonomic Classification of Lambdoid Bacteriophages

Pseudomonas Phage MD8: Genetic Mosaicism and Challenges of Taxonomic Classification of Lambdoid Bacteriophages

SGP-C: A Broad Host Range Temperate Bacteriophage; Against Salmonella gallinarum

Diversity of Pseudomonas aeruginosa Temperate Phages

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