Molecular characterization and expression analysis of two farnesyl pyrophosphate synthase genes involved in rubber biosynthesis in Hevea brasiliensis

ChunTai, Wu; Sun, Lina; Yu, Li; Zeng, Rizhong

doi:10.1016/j.indcrop.2017.06.042

Cited by 12 publications

(7 citation statements)

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“…HbPMD expression in the latex of newly tapped young H. brasiliensis trees from clone PR 107 at the 1st and 7th tappings after tree opening for tapping [ 25 ] and its expression in latex from this clone when it reached the second tapping year were all greater than in ‘RRIM 600’, ‘CATAS 73397’, ‘CATAS 72059’ or ‘CATAS 879, but there was no close correlation among the HbPMD gene expression levels. HbFPS1 ’s expression level in latex of the initially tapped young H. brasiliensis trees of ‘PR 107’ at the first tapping after opening of trees and HbFPS ’s expression in the latex of tapped Hevea trees from the same clone during its second tapping year were all greater compared with in ‘RRIM 600’, ‘CATAS 73397′, ‘CATAS 72059′ or ‘CATAS 879′, and there was a correlation among HbFPS expression levels ( R = 0.898, P < 0.05), which was not the case for HbFPS1 ’s expression at the 7th tapping or HbFPS2 ’s expression at the 1st and 7th tappings [ 34 ]. Thus, the dynamic changes of rubber synthesis-related gene mRNA expression levels varied with the clonal varieties, as well as the tapping times and genes.…”

Section: Discussionmentioning

confidence: 99%

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The relationship between latex metabolism gene expression with rubber yield and related traits in Hevea brasiliensis

ChunTai

Lan

et al. 2018

BMC Genomics

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BackgroundExpression patterns of many laticifer-specific gens are closely correlative with rubber yield of Hevea brasiliensis (para rubber tree). To unveil the mechanisms underlying the rubber yield, transcript levels of nine major latex metabolism-related genes, i.e., HMG-CoA synthase (HMGS), HMG-CoA reductase (HMGR), diphosphomevalonate decarboxylase (PMD), farnesyl diphosphate synthase (FPS), cis-prenyltransferase (CPT), rubber elongation factor (REF), small rubber particle protein (SRPP), dihydroxyacid dehydratase (DHAD) and actin depolymerizing factor (ADF), were dertermined, and the relationship between rubber yield with their expression levels was analysed.ResultsExcept HbHMGR1, HbPMD and HbDHAD, most of these genes were predominantly expressed in latex, and bark tapping markedly elevated the transcript abundance of the analyzed genes, with the 7th tapping producing the greatest expression levels. Both ethephon (ETH) and methyl jasmonate (MeJA) stimulation greatly induced the expression levels of the examined genes, at least at one time point, except HbDHAD, which was unresponsive to MeJA. The genes’ expression levels, as well as the rubber yields and two yield characteristics differed significantly among the different genotypes examined. Additionally, the latex and dry rubber yields increased gradually but the dry rubber content did not. Rubber yields and/or yield characteristics were significantly positively correlated with HbCPT, HbFPS, HbHMGS, HbHMGR1 and HbDHAD expression levels, negatively correlated with that of HbREF, but not significantly correlated with HbPMD, HbSRPP and HbADF expression levels. In addition, during rubber production, significantly positive correlations existed between the expression level of HbPMD and the levels of HbREF and HbHMGR1, between HbSRPP and the levels of HbHMGS and HbHMGR1, and between HbADF and HbFPS.ConclusionsThe up-regulation of these genes might be related to the latex production of rubber trees under the stress of bark tapping and latex metabolism. The various correlations among the genes implied that there are differences in their synergic interactions. Thus, these nine genes might be related to rubber yield and yield-related traits in H. brasiliensis, and this work increases our understanding of their complex functions and how they are expressed in both high-and medium-yield rubber tree varieties and low-yield wild rubber tree germplasm.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5242-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

“…Tender leaves, male and female flowers, stem bark and latex used for the qRT-PCR experiments were collected from seven-year-old untapped clonal rubber trees of ‘CATAS 73397’. ETH and MeJA treatments before opening the rubber tree for tapping and fresh latex collection and preservation were conducted as previously described [ 25 , 34 ].…”

Section: Methodsmentioning

confidence: 99%

The relationship between latex metabolism gene expression with rubber yield and related traits in Hevea brasiliensis

ChunTai

Lan

et al. 2018

BMC Genomics

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“…There are three acetyl‐CoA acetyltransferase genes, two HMGS genes and four HMG‐CoA reductase (HMGR) genes (Figure 4). All these MVA pathway genes are highly expressed in latex (Wu et al, 2017; see also Asawatreratanakul et al, 2003).…”

Section: Genes/proteins Involved In Rubber Biosynthesismentioning

confidence: 99%

Molecular markers to devise predictive models for juvenile selection in Hevea rubber

Priyadarshan

2022

Plant Breeding

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Natural rubber is extracted from the Hevea tree, through shaving the bark. As a major raw material source for many industries especially the tyre sector, Hevea rubber clones are intensively cultivated in the tropics. The world production of Hevea rubber is almost 13.64 million metric tonnes, and the productivity is around 1200 kg ha−1. However, the field production achieved so far is still well below the theoretical yield estimated (7000–12,000 kg ha−1). Even though this is a calculated estimate, efforts can be kept live to achieve this target, because Hevea rubber is being increasingly cultivated under several suboptimal environments after the 1980s. In this era of climate change, newer biotic and abiotic stresses encounter Hevea rubber culture that demands new clones at definite intervals. Hence, like any other species, Hevea rubber breeding is a continuous process in quest of new clones achieved through exploiting new sources of genetic variation. Hevea rubber breeding is to produce high‐yielding clones with good timber value. First priority is to explore and exploit genetic variation in clones, half‐sib, full‐sib and illegitimate (open‐pollinated) seedlings, where dry rubber yield and wood volume are the target traits. Next is to maximize genetic gains per unit time, through understanding juvenile–mature correlations and to practice selection of high‐yielding genotypes at the juvenile stage. Hevea rubber had a long breeding cycle of 33 years but could be reduced to 17 years after culling non‐essential steps of deriving clones. However, this could be reduced to 10 years, if a DNA marker‐assisted selection system is applied to seedlings. This system must also ensure significant juvenile–mature correlations on yield. This is a formidable task because under given circumstances, such a correlation is almost absent. However, this can be turned into a reality through employing yield‐related DNA markers with genomic selection (GS). The impact of DNA markers linked with REF, SRPP, acetyl‐CoA acetyltransferase, HMGS, HMGR and JAZ need to be specifically evaluated. GS that aims to improve quantitative traits by using genome‐wide marker data without requiring identification of markers associated with quantitative trait loci (QTLs) of interest can be used in Hevea also. Subsequently, by a predictive model, genomic estimated breeding values (GEBVs) can be calculated for untested individuals from a ‘testing population’. GS captures the total additive genetic variance with genome‐wide marker coverage and effect estimates. Therefore, selection of an individual without phenotypic data can be performed based on the individual's predicted breeding value. This review makes an assessment of the molecular markers in Hevea rubber and aims to streamline an efficient selection system to be applied onto seedlings through GS that owes a significant correlation with the mature phase.

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“…Genes of the MEP and MVA pathways from rubber-producing plants were cloned and functionally analyzed [84,85,86,87]. While MVA pathway was thought to be the main provider of the IPP monomer, there were different opinions about whether MEP pathway was directly involved in rubber biosynthesis.…”

Section: Biosynthesis Of Natural Rubber and The Involved Genes/promentioning

confidence: 99%

Biosynthesis of Natural Rubber: Current State and Perspectives

Men

Wang

Chen

et al. 2018

IJMS

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Natural rubber is a kind of indispensable biopolymers with great use and strategic importance in human society. However, its production relies almost exclusively on rubber-producing plants Hevea brasiliensis, which have high requirements for growth conditions, and the mechanism of natural rubber biosynthesis remains largely unknown. In the past two decades, details of the rubber chain polymerization and proteins involved in natural rubber biosynthesis have been investigated intensively. Meanwhile, omics and other advanced biotechnologies bring new insight into rubber production and development of new rubber-producing plants. This review summarizes the achievements of the past two decades in understanding the biosynthesis of natural rubber, especially the massive information obtained from the omics analyses. Possibilities of natural rubber biosynthesis in vitro or in genetically engineered microorganisms are also discussed.

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Molecular characterization and expression analysis of two farnesyl pyrophosphate synthase genes involved in rubber biosynthesis in Hevea brasiliensis

Cited by 12 publications

References 36 publications

The relationship between latex metabolism gene expression with rubber yield and related traits in Hevea brasiliensis

The relationship between latex metabolism gene expression with rubber yield and related traits in Hevea brasiliensis

Molecular markers to devise predictive models for juvenile selection in Hevea rubber

Biosynthesis of Natural Rubber: Current State and Perspectives

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