Molecular Characterization and Expression Profiles of Sp-uchl3 and Sp-uchl5 during Gonad Development of Scylla paramamosain

Abstract

Ubiquitin C-terminal hydrolases (UCHLs) are a subset of deubiquitinating enzymes, and are involved in numerous physiological processes. However, the role of UCHLs during gonad development has not been studied in crustaceans. In this study, we have first cloned and analyzed expression profiling of Sp-uchl3 and Sp-uchl5 genes from mud crab Scylla paramamosain. The full-length cDNA of Sp-uchl3 is of 1804 bp. Its expression level in the ovary was significantly higher than in other tissues (p < 0.01), and during gonadal development, its expression in both O1 and O5 stages was significantly higher than in the other three stages of ovaries (p < 0.05), while in T3 it was higher than in the former two stages of testes (p < 0.05). Meanwhile, the full-length cDNA of Sp-UCHL5 is 1217 bp. The expression level in the ovary was significantly higher than in other tissues (p < 0.01). Its expression in ovaries was higher than in testes during gonadal development (p < 0.05). The expression level in the O5 stage was the highest, followed by the O3 stage in ovarian development, and with no significant difference in the testis development (p > 0.05). These results provide basic data showing the role of Sp-UCHL3 and Sp-UCHL5 in the gonad development of the crab.

Comparative Transcriptome Analysis Reveals the Process of Ovarian Development and Nutrition Metabolism in Chinese Mitten Crab, Eriocheir Sinensis

“…UPP is involved in oocyte meiotic maturation as a key intracellular protein degradation system in the cell cycle ( Bebington et al, 2001 ). In crustaceans, Ubiquitin C-terminal hydrolases L3 ( UCHL3 ), UCHL5 , ubiquitin ribosomal protein S27 ( UbS27 ), ubiquitin ribosomal protein L40, and UPP system-related factors participate in the regulation of oogenesis ( Han et al, 2010 ; Wang et al, 2012 ; Han et al, 2018 ). In this study, we detected many genes in the pathway that changed in the ovary and hepatopancreas during ovarian development, such as UbS27a , ubiquitin-conjugating enzyme, ubiquitin carboxyl-terminal hydrolase isozyme L5, and ubiquitin-protein ligase.…”

Silencing UCHL3 enhances radio-sensitivity of non-small cell lung cancer cells by inhibiting DNA repair

“…UCHL3 is a subset of deubiquitinating enzymes, belonging to the ubiquitin c-terminal hydrolase family [ 19 ]. In recent years, the relationship between UCHL3 and DNA damage repair has received increased attention.…”

The Regulatory Mechanism of Sexual Development in Decapod Crustaceans

“…However, little information is available regarding the role of UCHLs in gonadal development of crustaceans. UCHLs have been identified only in S. paramamosain (Han et al, 2018). Han et al (2018) reported that the expression level of UCHL3 and UCHL5 in the ovary was significantly higher than in other tissues which provides basic data indicating that UCHL3 and UCHL5 may play important roles in the ovarian development of S. paramamosain.…”