Molecular characterization and functional activity of CXCL8_L3 in large yellow croaker Larimichthys crocea

Zhou, Shimin; Mu, Yinnan; Ao, Jingqun; Chen, Xinhua

doi:10.1016/j.fsi.2017.12.052

Cited by 39 publications

(15 citation statements)

References 33 publications

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“…SGIV was propagated in grouper spleen cells as previously described 50 , and the virus stock was stored at −80 °C until use. Macrophages were isolated from the head kidney tissues of L. crocea , as previously described 39 , and cultured at 28 °C in L-15 medium.…”

Section: Methodsmentioning

confidence: 99%

An improved genome assembly for Larimichthys crocea reveals hepcidin gene expansion with diversified regulation and function

Huo

Guan

et al. 2018

Commun Biol

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Larimichthys crocea (large yellow croaker) is a type of perciform fish well known for its peculiar physiological properties and economic value. Here, we constructed an improved version of the L. crocea genome assembly, which contained 26,100 protein-coding genes. Twenty-four pseudo-chromosomes of L. crocea were also reconstructed, comprising 90% of the genome assembly. This improved assembly revealed several expansions in gene families associated with olfactory detection, detoxification, and innate immunity. Specifically, six hepcidin genes (LcHamps) were identified in L. crocea, possibly resulting from lineage-specific gene duplication. All LcHamps possessed similar genomic structures and functional domains, but varied substantially with respect to expression pattern, transcriptional regulation, and biological function. LcHamp1 was associated specifically with iron metabolism, while LcHamp2s were functionally diverse, involving in antibacterial activity, antiviral activity, and regulation of intracellular iron metabolism. This functional diversity among gene copies may have allowed L. crocea to adapt to diverse environmental conditions.

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Section: Methodsmentioning

confidence: 99%

An improved genome assembly for Larimichthys crocea reveals hepcidin gene expansion with diversified regulation and function

Huo

Guan

et al. 2018

Commun Biol

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“…Several CXC-type chemokines, such as CXCL11 [23], CXCL8 [24], and CXCL12 [25] groups, exert chemotactic activity in bony fish. However, the CXCL20 group is part of the more recent chemokines whose function has not yet been investigated.…”

Section: Discussionmentioning

confidence: 99%

“…To date, the function of the CXCL11 [23], CXCL8 [24], and CXCL12 [25] groups has been widely studied in bony fish. Recently, many studies have indicated that bony fish CXCL20 group (especially CXCL20b) may play an important role in host defense against bacterial infections.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Antimicrobial Activity of the Teleost Chemokine CXCL20b

et al. 2020

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Fish are a potential source of diverse organic compounds with a broad spectrum of biological activities. Many fish-derived antimicrobial peptides and proteins are key components of the fish innate immune system. They are also potential candidates for development of new antimicrobial agents. CXCL20b is a grass carp (Ctenopharyngodon idella) CXC chemokine strongly transcribed at the early stage of bacterial infections, for which the immune role had not been reported to date. In the present study, we found that CXCL20b is a cationic amphipathic protein that displays potent antimicrobial activity against both Gram-positive and Gram-negative bacteria. The results of DiOC2(3) and atomic force microscopy (AFM) assays indicated that CXCL20b could induce bacterial membrane depolarization and disruption in a short time. By performing further structure-activity studies, we found that the antimicrobial activity of CXCL20b was mainly relative to the N-terminal random coil region. The central part of this cytokine representing β-sheet region was insoluble in water and the C-terminal α-helical region did not show an antimicrobial effect. The results presented in this article support the poorly understood function of CXCL20b, which fulfills an important role in bony fish antimicrobial immunity.

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“…CXCL8-L2 is a cyprinid-specific lineage and has been identified in common carp [ 14 , 15 ], zebra fish [ 24 ], and grass carp [ 11 , 25 ]. Meanwhile, CXCL8-L3 is a recently identified lineage, which has been reported in large yellow croaker [ 26 ] and rainbow trout [ 11 ]. While the majority of the previous studies were primarily focused on transcriptional responses of cxcl8 [ 12 , 17 , 22 , 27 , 28 ], some groups have also examined the biological functions of fish CXCL8, such as chemotaxis [ 11 , 13 , 14 , 15 , 18 , 19 , 20 , 21 , 23 , 26 , 29 , 30 , 31 , 32 , 33 ], cell proliferation [ 20 , 21 , 31 ], superoxide production/respiratory burst [ 15 , 22 , 23 , 26 ], and phagocytosis [ 30 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

“…In zebrafish, both Cxcl8-l1 and Cxcl8-l2 were required for normal neutrophil migratory behavior [ 24 ]. CXCL8-L3 from large yellow croaker was chemotactically active and increased the respiratory burst [ 26 ]. Besides these findings, the genomic arrangement of cxcl8 has not been investigated in a comparative context in detail to understand the genomic evolutionary aspects of cxcl8 [ 22 , 27 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Characterization of Two cxcl8 Homologs in Oplegnathus fasciatus: Genomic, Transcriptional and Functional Analyses

et al. 2020

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CXCL8 (interleukin-8, IL-8) is a CXC family chemokine that recruits specific target cells and mediates inflammation and wound healing. This study reports the identification and characterization of two cxcl8 homologs from rock bream, Oplegnathus fasciatus. Investigation of molecular signature, homology, phylogeny, and gene structure suggested that they belonged to lineages 1 (L1) and 3 (L3), and designated Ofcxcl8-L1 and Ofcxcl8-L3. While Ofcxcl8-L1 and Ofcxcl8-L3 revealed quadripartite and tripartite organization, in place of the mammalian ELR (Glu-Leu-Arg) motif, their peptides harbored EMH (Glu-Met-His) and NSH (Asn-Ser-His) motifs, respectively. Transcripts of Ofcxcl8s were constitutively detected by Quantitative Real-Time PCR (qPCR) in 11 tissues examined, however, at different levels. Ofcxcl8-L1 transcript robustly responded to treatments with stimulants, such as flagellin, concanavalin A, lipopolysaccharide, and poly(I:C), and pathogens, including Edwardsiella tarda, Streptococcus iniae, and rock bream iridovirus, when compared with Ofcxcl8-L3 mRNA. The differences in the putative promoter features may partly explain the differential transcriptional modulation of Ofcxcl8s. Purified recombinant OfCXCL8 (rOfCXCL8) proteins were used in in vitro chemotaxis and proliferation assays. Despite the lack of ELR motif, both rOfCXCL8s exhibited leukocyte chemotactic and proliferative functions, where the potency of rOfCXCL8-L1 was robust and significant compared to that of rOfCXCL8-L3. The results, taken together, are indicative of the crucial importance of Ofcxcl8s in inflammatory responses and immunoregulatory roles in rock bream immunity.

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Molecular characterization and functional activity of CXCL8_L3 in large yellow croaker Larimichthys crocea

Cited by 39 publications

References 33 publications

An improved genome assembly for Larimichthys crocea reveals hepcidin gene expansion with diversified regulation and function

An improved genome assembly for Larimichthys crocea reveals hepcidin gene expansion with diversified regulation and function

Characterization and Antimicrobial Activity of the Teleost Chemokine CXCL20b

Comparative Characterization of Two cxcl8 Homologs in Oplegnathus fasciatus: Genomic, Transcriptional and Functional Analyses

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