Molecular characterization and functional analysis of daf‐16‐2b gene in the pine wood nematode, Bursaphelenchus xylophilus

Abstract

The Forkhead box O transcription factor DAF-16 is an important downstream transcription factor in the well-conserved insulin signalling (IIS) pathway in Caenorhabditis elegans. The nuclear localization of DAF-16 can activate a series of genes that mediate oxidative stress, heat shock, xenobiotic responses, innate immunity, metabolism and autophagy. In this study, a Bxdaf-16-2b gene encoding 510 amino acids was

Effect of Associated Bacteria GD1 on the Low-Temperature Adaptability of Bursaphelenchus xylophilus Based on RNA-Seq and RNAi

“…This phenomenon is called RNAi [ 34 ]. In recent years, RNAi technology has been frequently used to assess PWN gene function, such as identifying the role of cathepsin L-like cysteine protease in PWN reproduction and pathogenicity [ 35 ], the role of Bx-daf-22 in PWN mate attraction [ 36 ] and the role of daf-16-2b in PWN detoxification metabolism processes [ 37 ]. RNAi technology has also played an important role in the study of the low-temperature adaptation mechanism of PWNs.…”