Molecular characterization and functionality of rumen-derived extracellular vesicles using a Caenorhabditis elegans animal model

Choi, Hyejin; Mun, Daye; Ryu, Sangdon; Kwak, Mi Kyung; Kim, Bum-Keun; Park, Dong-Jun; Oh, Sangnam; Kim, Young-Hoon

doi:10.5187/jast.2022.e124

Cited by 7 publications

(5 citation statements)

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“…The C. elegans strain fer-15;ferm-1 mutant was used, and they were routinely maintained on Nematode Growth Medium (NGM) agar plates. Eggs were extracted in sodium hypochlorite–sodium hydroxide solution and synchronized L1 worms were grown on NGM agar plates seeded with Escherichia coli OP50 at 25 °C to obtain sterile L4/young adult worms [ 57 ]. To perform a lifespan assay with series of methionine samples, the L1 or L4 stage of C. elegans strain fer-15;fem-1 were individually transferred with a platinum wire onto 35-mm-diameter NGM agar plates.…”

Section: Methodsmentioning

confidence: 99%

Dietary L-Methionine modulates the gut microbiota and improves the expression of tight junctions in an in vitro model of the chicken gastrointestinal tract

Kwak,

Kang,

Eor

et al. 2024

anim microbiome

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Background The poultry industry encounters a number of factors that affect growth performance and productivity; nutrition is essential for sustaining physiological status and protecting against stressors such as heat, density, and disease. The addition of vitamins, minerals, and amino acids to the diet can help restore productivity and support the body’s defense mechanisms against stress. Methionine (Met) is indispensable for poultry’s energy metabolism, physiology, performance, and feed utilization capacity. Through this study, we aimed to examine the physiological effects of methionine supplementation on poultry as well as alterations of intestinal microbiome. Methods We utilized the DL- and L- form of methionine on Caenorhabditis elegans and the FIMM (Fermentor for intestine microbiota model) in-vitro digesting system. A genomic-analysis of the transcriptome confirmed that methionine supplementation can modulate growth-related physiological metabolic pathways and immune responses in the host poultry. The C. elegans model was used to assess the general health benefits of a methionine supplement for the host. Results Regardless of the type or concentration of methionine, supplementation with methionine significantly increased the lifespan of C. elegans. Feed grade L-Methionine 95%, exhibited the highest lifespan performance in C. elegans. Methionine supplementation increased the expression of tight junction genes in the primary intestinal cells of both broiler and laying hens, which is directly related to immunity. Feed grade L-Methionine 95% performed similarly or even better than DL-Methionine or L-Methionine treatments with upper doses in terms of enhancing intestinal integrity. In vitro microbial cultures of healthy broilers and laying hens fed methionine revealed changes in intestinal microflora, including increased Clostridium, Bacteroides, and Oscillospira compositions. When laying hens were given feed grade L-Methionine 95% and 100%, pathogenic Campylobacter at the genus level was decreased, while commensal bacteria were increased. Conclusions Supplementation of feed grade L-Methionine, particularly L-Methionine 95%, was more beneficial to the host poultry than supplementing other source of methionine for maintaining intestinal integrity and healthy microbiome.

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Section: Methodsmentioning

confidence: 99%

Dietary L-Methionine modulates the gut microbiota and improves the expression of tight junctions in an in vitro model of the chicken gastrointestinal tract

Kwak,

Kang,

Eor

et al. 2024

anim microbiome

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“…The live bacterial lawn was prepared for C. elegans feeding by centrifuging the bacterial pellet at 13,000 rpm for 1 minute, washing it twice with sterile M9 buffer (3 g KH 2 PO 4 , 6 g Na 2 HPO 4 , and 5 g NaCl mixed in 1 L distilled water, autoclaved), and then adding 1 mL of 1 M MgSO 4 (Sigma‒Aldrich, St. Louis, MO, USA). The bacterial pellet was then concentrated to a final concentration of 2.5 mg/L (wet weight) in M9 buffer and suspended on NGM plates [3.5 g Bacto Peptone (BD Difco), 3 g NaCl (Sigma‒Aldrich), and 20 g agar (BD Difco)] ( 11 ).…”

Section: Methodsmentioning

confidence: 99%

“…The transparency of C. elegans ’ body provides a distinct advantage, allowing for easy observation of lipid droplets and other potentially hazardous accumulations. This characteristic makes C. elegans a preferred model for investigating various aspects of health, including obesity, feeding behaviors, satiety, and metabolic disorders ( 11 , 12 ). The combination of these attributes positions C. elegans as a practical and informative model for the initial screening of potential probiotics.…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation with probiotics promotes weight loss by reshaping the gut microbiome and energy metabolism in obese dogs

Kang,

Kwak,

Lee

et al. 2024

Microbiol Spectr

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Obesity and overweight among companion animals are significant concerns, paralleling the issues observed in human populations. Recent research has highlighted the potential benefits of various probiotics in addressing weight-related changes, obesity, and associated pathologies. In this study, we delved into the beneficial probiotic mechanisms in high-fat-induced obese canines, revealing that Enterococcus faecium IDCC 2102 (IDCC 2102) and Bifidobacterium lactis IDCC 4301 (IDCC 4301) have the capacity to mitigate the increase in body weight and lipid accumulation in obese canines subjected to a high-fat diet and hyperlipidemic Caenorhabditis elegans ( C. elegans ) strain VS29. Both IDCC 2102 and IDCC 4301 demonstrated the ability to reduce systemic inflammation and hormonal disruptions induced by obesity. Notably, these probiotics induced modifications in the microbiota by promoting lactic acid bacteria, including Lactobacillaceae , Ruminococcaceae , and S24-7 , with concomitant activation of pyruvate metabolism. IDCC 4301, through the generation of bacterial short-chain fatty acids and carboxylic acids, facilitated glycolysis and contributed to ATP synthesis. Meanwhile, IDCC 2102 produced bacterial metabolites such as acetic acid and butyric acid, exhibiting a particular ability to stimulate dopamine synthesis in a canine model. This stimulation led to the restoration of eating behavior and improvements in glucose and insulin tolerance. In summary, we propose novel probiotics for the treatment of obese animals based on the modifications induced by IDCC 2102 and IDCC 4301. These probiotics enhanced systemic energy utilization in response to high caloric intake, thereby preventing lipid accumulation and restoring stability to the fecal microbiota. Consequently, this intervention resulted in a reduction in systemic inflammation caused by the high-fat diet. IMPORTANCE Probiotic supplementation affected commensal bacterial proliferation, and administering probiotics increased glycolysis and activated pyruvate metabolism in the body, which is related to propanate metabolism as a result of pyruvate metabolism activation boosting bacterial fatty acid production via dopamine and carboxylic acid specialized pathways, hence contributing to increased ATP synthesis and energy metabolism activity.

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“…C. elegans, an extensively utilized genetic model organism capable of mimicking numerous human diseases, holds considerable value for in vivo studies at both metabolic and genetic levels [25,26]. This study employed the C. elegans lifespan assay to scrutinize the toxicity of distinct antler velvet extracts, including FAV, FFA, DAV, and CFA.…”

Section: Antler Velvet Extract Increases the Lifespan Of C Elegansmentioning

confidence: 99%

A comprehensive assessment of immunomodulatory potentials of Korean antler velvet extract in mouse and neurodegenerative Caenorhabditis elegans models

Kang,

Lee,

Eor

et al. 2024

J Anim Sci Technol

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This study conducts a comprehensive analysis of deer antler velvet's impact, with a specific emphasis on mouse models and in vitro experiments. The study navigates the intricacies of antler velvet's variability, encompassing considerations of drying methods, extraction techniques, and anatomical regions of antlers.Employing a diverse array of processing methods, this study prioritizes both food safety and the consistent intake of deer antler velvet extracts. The study scrupulously evaluates toxicity and immune-boosting properties through exhaustive assessments, utilizing Caenorhabditis elegans, immunosuppressive mouse models, and immune cells. The study unfolds the repercussions of deer antler velvet extract on the lifespan, neuromuscular functions, and cognitive abilities of C. elegans. Additionally, this study explores the extract's potential to alleviate toxicity induced by cyclophosphamide (CPA) in a mouse model, with a focus on inflammation, metabolic disorders, and gut microbiota composition. The antler velvet extract prevents immune dysregulation caused by CPA treatment by ameliorating systemic inflammation and restoring energy metabolism. Furthermore, antler velvet extract treatment significantly transforms the gut microbiota of an immunosuppressive mouse model by fostering the proliferation of commensal bacteria such as Lactobacillus, Akkermansia, and Lachnospiraceae at the genus level. Moreover, antler velvet treatment enhances the activity of natural killer cells against YAC-1 lymphoma while tempering overactivity among immune cells to baseline levels. In conclusion, this study provides nuanced insights into the diverse benefits of antler velvet extract, encouraging sustained research to unveil its complete potential in the realms of mouse models and in vitro experiments.

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Molecular characterization and functionality of rumen-derived extracellular vesicles using a Caenorhabditis elegans animal model

Abstract: Molecular characterization and functionality of rumen-derived extracellular vesicles using a Caenorhabditis elegans animal model Running

Cited by 7 publications

References 46 publications

Dietary L-Methionine modulates the gut microbiota and improves the expression of tight junctions in an in vitro model of the chicken gastrointestinal tract

Dietary L-Methionine modulates the gut microbiota and improves the expression of tight junctions in an in vitro model of the chicken gastrointestinal tract

Dietary supplementation with probiotics promotes weight loss by reshaping the gut microbiome and energy metabolism in obese dogs

A comprehensive assessment of immunomodulatory potentials of Korean antler velvet extract in mouse and neurodegenerative Caenorhabditis elegans models

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