Molecular characterization and genetic diversity analysis of selected maize inbreds using SSR markers

Sharma, T. R.; Kumar, Akhilesh; Dwivedi, SL; Vyas, R.P.

doi:10.22438/jeb/39/2/mrn-394

Cited by 5 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, 95 inbred lines were investigated using 1,536 SNPs by Hao et al [50]. Sharma et al (2018) detected 191 alleles showing a range of two to six alleles per marker and an average value of 3.82 alleles per locus. The PIC value showed a range of 0.050 (umc1069) to 0.817 (bnlg1823) with an average value of 0.585, indicating the efficiency of markers to study the level of polymorphism available in the maize inbred lines [115].…”

Section: Genetic Diversitymentioning

confidence: 99%

Untitled

2021

OBM Genet

View full text Add to dashboard Cite

The editors of OBM Genetics would like to express their sincere gratitude to the following reviewers for assessing manuscripts in 2021. We greatly appreciate the contribution of expert reviewers, which is crucial to the journal's editorial process. We aim to recognize reviewer contributions through several mechanisms, of which the annual publication of reviewer names is one. Reviewers receive a voucher entitling them to a discount on their next LIDSEN publication and can download a certificate of recognition directly from our submission system. Additionally, reviewers can sign up to the service Publons (https://publons.com) to receive recognition. Of course, in these initiatives we are careful not to compromise revi ewer confidentiality. Many reviewers see their work as a voluntary and often unseen part of their role as researchers. We are grateful to the time reviewers donate to our journals and the contribution they make.The editors of OBM Genetics would like to express their sincere gratitude to the following reviewers for assessing manuscripts in 2021.We greatly appreciate the contribution of expert reviewers, which is crucial to the journal's editorial process. We aim to recognize reviewer contributions through several mechanisms, of which the annual publication of reviewer names is one. Reviewers receive a voucher entitling them to a discount on their next LIDSEN publication and can download a certificate of recognition directly from our submission system. Additionally, reviewers can sign up to the service Publons (https://publons.com) to receive recognition. Of course, in these initiatives we are careful not to

show abstract

Section: Genetic Diversitymentioning

confidence: 99%

Untitled

2021

OBM Genet

View full text Add to dashboard Cite

show abstract

“…Inbreds P4, P7, P9 and P10 fall in cluster II, while Cluster III contained the lines P5 and P6. Among the molecular markers, use of SSR markers for heterotic grouping in maize has proven valuable (Akinwale et al, 2014;Rajendran et al, 2014;Sharma et al, 2017).…”

Section: Plant Materials and Field Evaluationmentioning

confidence: 99%

Heterotic grouping of late-maturing maize inbred lines based on combining ability and molecular marker studies

R.T.¹,

Gadge²,

Bhat³

et al. 2019

JEB

View full text Add to dashboard Cite

To examine concurrence between yield based and SSR marker based heterotic grouping of latematuring maize inbred lines. A total of 45 F 1 crosses derived from 10x10 diallel mating scheme were evaluated along with their parents and hybrid checks across two locations (New Delhi and Dharawada).To group the inbred lines at molecular level, 35 polymorphic SSR primers were used for PCR amplification of repeat sequences from the genomic DNA of each inbred isolated by CTAB method. Cluster analysis was carried out by using NTSYS-pc-2.02. Analysis of variance revealed significant (P<0.01) variation among the parents and their hybrids across two locations for all the traits. The yield SCA classified 10 latematuring maize inbred lines into t h r e e h e t e r o t i c g r o u p s. Similarly, molecular genetic d i v e r s i t y a n a l y s i s a l s o categorized the inbred lines into three major clusters. However, correlations between SCA effects and genetic distance were low (r=0.161) for grain yield. Heterosis prediction with the help of molecular markers alone was not found effective. Further, field-testing can be complimented with molecular markers for the elimination of inferior crosses.

show abstract

“…Genotyping techniques by using genetic markers have allowed assessment of the genetic diversity among maize inbred lines to synthesize most heterozygotic hybrid combinations (35). Among the various types of DNA-based markers, microsatellites or simple sequence recurrent (SSR), which are short sequences containing tandemly repeated copies of one to six nucleotide fragments, are currently considered as the molecular markers of choice and used extensively to assess genetic diversity (34). SSRs rapidly adapted by plant researchers due to their simplicity, high levels of polymorphism, high reproducibility, and codominant inheritance patterns (21).…”

mentioning

confidence: 99%

The Genetic Diversity of Maize Inbred Lines Grown in Iraq Using Simple Sequence Recurrent (Ssr) Markers

Alaa K.H. Al-Hazemawi,

Dhia S. Hassawi,

Abbas A. mohammed

2024

Iraqi J. Agric. Sci.

View full text Add to dashboard Cite

This study was conducted to determine the genetic variability among maize inbred lines grown in Iraq by using Simple Sequence Recurrent (SSR) markers. For estimating the genetic relationships, 10 SSR primers were used with 10 maize inbred lines. Two hundred and sixteen alleles (bands) with a range of 13 to 41 were identified for the SSR loci. Polymorphism information content (PIC) of the 10 SSR loci ranged from 0.60% to 100%. Primers ‘umc1566’, ‘umc1542’, and ‘umc2189’ showed the maximum PIC value; by contrast, ‘umc2225’showed the lowest PIC value. The 10 maize inbreds were clustered based on the matrix of genetic similarity using the UPGMA algorithm. Cluster analysis placed the inbreds lines in three clusters based on SSR data. The studied inbreds lines divided to groups and subgroups with cluster analysis that revealed agreement with their geographical origin. The results indicated that SSR marker has a high degree of polymorphism that allows efficient identification of maize genotypes, and this could be used in determining their heterotic groups.

show abstract

Molecular characterization and genetic diversity analysis of selected maize inbreds using SSR markers

Cited by 5 publications

References 10 publications

Untitled

Untitled

Heterotic grouping of late-maturing maize inbred lines based on combining ability and molecular marker studies

The Genetic Diversity of Maize Inbred Lines Grown in Iraq Using Simple Sequence Recurrent (Ssr) Markers

Contact Info

Product

Resources

About