Molecular characterization and genetic variability at κ-casein gene (CSN3) in camels

Pauciullo, Alfredo; Shuiep, E. S.; Cosenza, G.; Ramunno, L.; Erhardt, G.

doi:10.1016/j.gene.2012.10.083

Cited by 30 publications

(41 citation statements)

References 36 publications

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“…The nucleotide sequences of κ-CN alleles C and T were submitted to GenBank with the accession numbers; KU055605 and KU055606, respectively. Pauciullo et al (2013) reported the same SNPT>C in exon 1 of C. dromedaries k-CN after the digestion of amplified fragment with AluI restriction enzyme in four Sudanese breeds. They detected three different genotypes; CC (18.09%), TT (42.55%) and CT (39.36%).…”

Section: Resultsmentioning

confidence: 77%

“…The camel populations in Somalia and Sudan constitute a half of world camel populations (Pauciullo et al, 2013). The camel population in Egypt was estimated to be 120.000 headsand its ecotypes serve numerous functions in their respective production systems (e.g.…”

Section: Resultsmentioning

confidence: 99%

“…The primers used in this study amplified 488-bp fragments (Pauciullo et al, 2013) which spans from -137 bp of 5'-flanking region to +351 bp of the camel κ-CN gene. The amplified fragments were digested with two different restriction enzymes; SmlI and AluI.…”

Section: Resultsmentioning

confidence: 99%

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Genetic Variations in Two Casein Genes Among Maghrabi Camels Reared in Egypt

Othman¹,

Nowier²,

El-Denary³

2016

Biosci., Biotech. Res. Asia

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Camels play an important socio-economic role within the pastoral and agricultural system in the dry and semidry zones of Asia and African where they are dual purpose animals (meat and milk). In spite of the effective role of casein genes with their polymorphisms on quantitative traits and technological properties of milk, the studies on genetic polymorphism of camel milk genes are limited. This work aimed to identify the genetic polymorphisms and SNPs of two casein genes in Maghrabi camel breed in Egypt. The amplified fragments at 488-bp of κ κ κ κ κ-CN gene were digested with AluI endonuclease. The results showed the presence of three genotypes; CC (12%), TT (48%) CT (40%). The sequence analysis of two detected alleles declared the presence of a SNP (C→ → → → →T) at position 121 in amplified fragments. The nucleotide sequences of κ κ κ κ κ-CN alleles C and T were submitted to GenBank with accession numbers; KU055605 and KU055606, respectively. The primers used in this study amplified 942-bp fragments of ás1-casein gene. The results of SmlI digestion did not showed any restriction site whereas the digestion with AluI endonuclease revealed the presence of two restriction sites AG^CT at positions 68^69 and 631^632 in amplified fragments. The nucleotide sequence of monomorphic α α α α αs1-casein gene was submitted to GenBank with accession number KU145820.In conclusion, the genetic characterization of genes associated with milk yield and composition in camel is considered an essential step towards its genetic improvement through the selection of superior animals depending on the favorable alleles and genotypes; marker assisted selection (MAS).

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Section: Resultsmentioning

confidence: 77%

Section: Resultsmentioning

confidence: 99%

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Genetic Variations in Two Casein Genes Among Maghrabi Camels Reared in Egypt

Othman¹,

Nowier²,

El-Denary³

2016

Biosci., Biotech. Res. Asia

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“…) and non‐ruminant (Pauciullo et al . , ) species, the buffalo PRLR gene also is characterized by the presence of several retroposons located in the introns, representing about 13% of the entire gene. According to Lenstra et al .…”

Section: Resultsmentioning

confidence: 99%

Remarkable genetic diversity detected at river buffalo prolactin receptor (PRLR) gene and association studies with milk fatty acid composition

et al. 2018

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Prolactin is an anterior pituitary peptide hormone involved in many different endocrine activities and is essential for reproductive performance. This action is mediated by its receptor, the prolactin receptor, encoded by the PRLR gene. In this study, we sequenced and characterized the Mediterranean river buffalo PRLR gene (from exon 3 to 10), and we found remarkable genetic diversity. In particular, we found 24 intronic polymorphisms and 13 exonic SNPs, seven of which were non-synonymous. Furthermore, the polymorphisms identified in the 3'-UTR were investigated to establish their possible influence on microRNA binding sites. Considering all the amino acid changes and the observed allelic combinations, it is possible to deduce at least six different translations of the buffalo prolactin receptor and, consequently, the presence at the PRLR gene of at least six alleles. Furthermore, we identified a deletion of a CACTACC heptamer between nucleotides 1102 and 1103 of exon 10 (3'-UTR), and we developed an allele-specific PCR to identify the carriers of this genetic marker. Finally, the SNP g.11188A>G, detected in exon 10 and responsible for the amino acid replacement p.His328Arg, was genotyped in 308 Italian Mediterranean river buffaloes, and an association study with milk fat traits was carried out. The statistical analysis showed a tendency that approached significance for the AA genotype with higher contents of odd branched-chain fatty acids. Thus, our results suggest that the PRLR gene is a good candidate for gene association studies with qualitative traits related to buffalo milk production.

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“…Moreover unlike cow milk, camel milk is reported to have antidiabetic (Pauciullo et al, 2012) and antihypertensive effects. Camel milk fat lacks -lactoglobulin and is rich in immunoglobulins which are compatible with human milk (Shamsia, 2009).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and genetic variability of Alpha Casein gene, CSN1S1 in Bikaneri camel (Camelus dromedarius) milk

Singh

Jayakumar

Dixit

et al. 2018

IJAR

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Camel milk is an important protein source for the nomadic communities living in the arid lands of the world, In recent years there has been an increase in consumption of non-bovine milk as an alternative protein source for humans. Camel milk seems to be containing larger amount of total proteins, such as lactoferrin and immunoglobulins as compared to the cow milk, which may be responsible for the better antimicrobial properties. The casein fraction of milk proteins consists of four caseins, namely s1-casein, s2-casein, -casein, and -casein. Casein genetic polymorphisms are important due to their effects on quantitative traits and technological properties of milk. This work was designed to study occurrence of polymorphism of -casein in native Bikaneri camel (Camelus dromedarius) raw milk sample and to characterize these variants on molecular level.

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Molecular characterization and genetic variability at κ-casein gene (CSN3) in camels

Cited by 30 publications

References 36 publications

Genetic Variations in Two Casein Genes Among Maghrabi Camels Reared in Egypt

Genetic Variations in Two Casein Genes Among Maghrabi Camels Reared in Egypt

Remarkable genetic diversity detected at river buffalo prolactin receptor (PRLR) gene and association studies with milk fatty acid composition

Molecular characterization and genetic variability of Alpha Casein gene, CSN1S1 in Bikaneri camel (Camelus dromedarius) milk

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