Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana

Zhang, Liang; Meng, Fei; Li, Yuzhen; Kang, Mingjiang; Guo, Xingqi; Xu, Baohua

doi:10.5483/bmbrep.2012.45.5.293

Cited by 11 publications

(13 citation statements)

References 26 publications

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“…Tissue-specific expression analysis showed that AccMKK6 is expressed in various tissues but is most highly expressed in the thorax and epidermis. Similarly, the highest expression of Accp38b has been detected in the thorax [34]. The epidermis acts as the exoskeleton, conferring physical stability, and participates in the responses to various environmental stresses [41].…”

Section: Discussionmentioning

confidence: 99%

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Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Wang

Cui

et al. 2018

Bioscience Reports

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Mitogen-activated protein kinase kinases (MKKs) are important components of the MAPK signaling pathways, which play a key role in responding to stress and inflammatory stimuli. Here, a new MKK gene, AccMKK6, was identified and functionally analyzed in Apis cerana cerana. Real-time quantitative PCR (qPCR) and Western blot analysis demonstrated that the AccMKK6 expression level was up-regulated by several environmental stresses. Moreover, the knockdown of AccMKK6 by RNA interference technology altered the expression levels of some antioxidant genes. In addition, the knockdown of AccMKK6 resulted in increased malonyldialdehyde (MDA) concentration and decreased antioxidant-related enzymes activity in honeybees. To explore the MAPK signaling pathways involved in AccMKK6, we identified the transcription factor kayak in A. cerana cerana. We analyzed the interactions of AccMKK6, Accp38b, and Acckayak using the yeast two-hybrid system. AccMKK6 and Acckayak showed similar expression profiles after several stress treatments. In addition, the expression level of Acckayak was significantly increased when AccMKK6 was silenced. Therefore, we speculate that AccMKK6 may be involved in the MAPK cascades, which play a crucial role in counteracting oxidative stress caused by external stimuli.

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Section: Discussionmentioning

confidence: 99%

“…The typical MAPK member Accp38b (GenBank accession no. GU321334) was isolated as previously described [34] and ligated into the pGBKT7 vector. The ORFs of AccMKK6 and Acckayak were cloned and fused to the pGADT7 vector.…”

Section: Methodsmentioning

confidence: 99%

Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Wang

Cui

et al. 2018

Bioscience Reports

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“…). However, the higher or lower expression level of p38 is different in different animals (Livak & Schmittgen ; Hansen & Jørgensen ; Cai, Huang, Wei, Huang, Ye, Fu & Qin ; Zhang, Meng, Li, Kang, Guo & Xu ). This experiment found that the expression of Es‐p38 in muscle is significantly higher than other tissues, and it is also higher in hepatopancreas, intestine, testis, ovary, accessory gonad, but lower in other tissues.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and characterization ofp38gene in the Chinese Mitten Crab,Eriocheir sinensis

et al. 2014

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p38 is an important component of the MAPK signaling pathways as a Ser/Thr protein kinase. We cloned the full length p38 (Es-p38) cDNA sequence in Eriocheir Sinensis which contained a 1098nucleotide ORF that encoded a protein of 365 amino acids. Es-p38 protein has a p38 MAPK functional domain (STKc_p38), it is a typical serine/threonine kinase and processes all p38 protein's conservative T-G-Y motif. Tissue expression of the Es-p38 gene and expression during testis development stages were determined by quantitative real-time PCR analysis. The results show that Es-p38 gene is expressed in various tissues, but it expresses higher in the muscle, hepatopancreas, intestine, ovary and testis. The expression level of Es-p38 gene at different stages of testis development shows no significant difference between the spermatocyte phase (July-August) and spermatid stage (August-October), but it rises significantly in the mid-late period of sperm stage (December-January). This changing trend has a positive relationship with the E. Sinensis gonad development and reproduction stage, which preliminarily shows that Es-p38 may function in the E. Sinensis testis development and the process of spermatogenesis.

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“…AccMGST2 expression was the highest in the head of A. cerana cerana , followed by the muscle (Figure 4). The tissue expression characteristics of AccMGST2 were similar to those of the Accp38b gene from A. cerana cerana (Zhang et al, 2012), and AccMGST2 and Accp38b may have synergistic effects. Additionally, the tissue‐specific expression of one gene is related to its biological function.…”

Section: Discussionmentioning

confidence: 59%

Identification of an MGST2 gene and analysis of its function in antioxidant processes in Apis cerana cerana

Zhao

Cui

et al. 2021

Arch Insect Biochem Physiol

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MGST2 is a member of the MAPEG superfamily, which participates in LTC4 synthesis and plays important roles in the regulation of the oxidative stress pathway and some diseases. Here, we isolated a previously uncharacterized gene in Apis cerana cerana named AccMGST2 by reverse transcription‐polymerase chain reaction. The biological characteristics of AccMGST2 were analyzed by bioinformatics. The amino acid sequence similarity between AccMGST2 and AmMGST2 of Apis mellifera reached 96.08%. The expression characteristics of AccMGST2 were explored in several tissues. The quantitative real‐time polymerase chain reaction results showed that the AccMGST2 gene was highly expressed in the head and muscle and that AccMGST2 expression responded to oxidative stress caused by different abiotic stresses. AccMGST2 was silenced using RNA interference, which decreased the expression levels of some MAPK and antioxidant genes. Therefore, we conclude that AccMGST2 is involved in the regulation of oxidative stress in A. cerana cerana.

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Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana

Cited by 11 publications

References 26 publications

Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Molecular cloning and characterization ofp38gene in the Chinese Mitten Crab,Eriocheir sinensis

Identification of an MGST2 gene and analysis of its function in antioxidant processes in Apis cerana cerana

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