Molecular characterization and phylogenetic analysis of transmissible gastroenteritis virus HX strain isolated from China

Hu, Xiaoliang; Li, Nannan; Tian, Zhen; Yin, Xin; Qu, Liandong; Qu, Juanjuan

doi:10.1186/s12917-015-0387-8

Cited by 35 publications

(63 citation statements)

References 17 publications

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“…Piglets were fed a mixture of skim milk powder (Inner Mongolia Yi Li Industrial Group Co., Ltd, China) with warm water. Prior to inoculation, piglets were confirmed negative for the major porcine enteric viruses (PDCoV, PEDV, TGEV, PRoV) by testing of rectal swabs using specific RT-PCR according to previously described method (Hu, Li et al, 2015;Jeong et al, 2009;Saeng-Chuto et al, 2017). After 1-day acclimation, piglets in group 1 were orally inoculated with 5 ml of maintenance medium and served as uninfected controls.…”

Section: Experimental Infection With the Peav Gds04 Strain In Newbomentioning

confidence: 99%

Isolation and characterization of a highly pathogenic strain ofPorcine enteric alphacoronaviruscausing watery diarrhoea and high mortality in newborn piglets

Zhang

Gong

et al. 2018

Transbound Emerg Dis

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Porcine enteric alphacoronavirus (PEAV) was first discovered in China in February 2017, and the origin and virulence of this novel porcine coronavirus were not fully characterized. Here, we isolated a strain of PEAV, named GDS04 that is identified by immunofluorescence and typical crown-shaped particles observed with electron microscopy. Genomic analysis reveals that PEAV GDS04 shares a close relationship with SADS-CoV and SeACoV. Furthermore, newborn piglets orally challenged with PEAV GDS04 developed typical clinical symptoms as watery diarrhoea in neonatal piglets. Viral RNA was detected in faeces and various tissues of the infected piglets. Moreover, macroscopic and microscopic lesions in whole intestinal tract were observed, and viral antigen could be detected in the small intestines by immunohistochemical staining and electron microscopy. Importantly, the mortality rate of inoculated-newborn piglets was 100% and half of the cohabiting piglets died. Collectively, we demonstrate that PEAV is highly pathogenic in newborn piglets.

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Section: Experimental Infection With the Peav Gds04 Strain In Newbomentioning

confidence: 99%

Isolation and characterization of a highly pathogenic strain ofPorcine enteric alphacoronaviruscausing watery diarrhoea and high mortality in newborn piglets

Zhang

Gong

et al. 2018

Transbound Emerg Dis

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“…To detect PDCoV genome in collected fecal samples, the previously reported RT-PCR primers (41 F: 5'-TTT CAGGTGCTCAAAGCTCA-3' and 735R: 5'-GCGAAA AGCATTTCCTGAAC-3') targeting the nucleocapsid (N) gene with reaction conditions (50°C for 30 min and 95°C for 15 min for the reverse transcription reaction, followed by 40 cycles of PCR amplification at 95°C for 15 s, 55°C for 45 s, and 72°C for 1 min, with a final extension at 72°C for 7 min) were used [15]. In addition, molecular detection of the three diarrhea-related enteric viruses (Porcine epidemic diarrhea virus, PEDV; Porcine transmissible gastroenteritis virus, TGEV; Porcine rotavirus group C, Rota C) was performed in accordance with previous methods [20][21][22] for further evaluation of the possible co-infection status with PDCoV in investigated pig samples.…”

Section: Reverse Transcription Polymerase Chain Reaction (Rt-pcr) Detmentioning

confidence: 99%

Occurrence and sequence analysis of porcine deltacoronaviruses in southern China

Zhai

Wei

et al. 2016

Virol J

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Background: Following the initial isolation of porcine deltacoronavirus (PDCoV) from pigs with diarrheal disease in the United States in 2014, the virus has been detected on swine farms in some provinces of China. To date, little is known about the molecular epidemiology of PDCoV in southern China where major swine production is operated. Results: To investigate the prevalence of PDCoV in this region and compare its activity to other enteric disease of swine caused by porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis coronavirus (TGEV), and porcine rotavirus group C (Rota C), 390 fecal samples were collected from swine of various ages from 15 swine farms with reported diarrhea. Fecal samples were tested by reverse transcription-PCR (RT-PCR) that targeted PDCoV, PEDV, TGEV, and Rota C, respectively. PDCoV was detected exclusively from nursing piglets with an overall prevalence of approximate 1.28 % (5/390), not in suckling and fattening piglets. Interestingly, all of PDCoV-positive samples were from 2015 rather than 2012-2014. Despite a low detection rate, PDCoV emerged in each province/region of southern China. In addition, compared to TGEV (1.54 %, 5/390) or Rota C (1.28 %, 6/390), there were highly detection rates of PEDV (22.6 %, 88/390) in those samples. Notably, all five PDCoV-positive piglets were co-infected by PEDV. Furthermore, phylogenetic analysis of spike (S) and nucleocapsid (N) gene sequences of PDCoVs revealed that currently circulating PDCoVs in southern China were more closely related to other Chinese strains of PDCoVs than to those reported in United States, South Korea and Thailand. Conclusions: This study demonstrated that PDCoV was present in southern China despite the low prevalence, and supported an evolutionary theory of geographical clustering of PDCoVs.

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“…Fifteen primer pairs were designed based on the conserved regions of CCoV strain TN-449 (Table S1). The RT-PCR assay was performed as described previously [13].…”

Section: Isolation Identification and Purification Of The Virusmentioning

confidence: 99%

Molecular characterization of HLJ-073, a recombinant canine coronavirus strain from China with an ORF3abc deletion

et al. 2019

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Canine enteric coronaviruses (CCoVs) are important enteric pathogens of dogs. CCoVs with different variations are typically pantropic and pathogenic in dogs. In this study, we isolated a CCoV, designated HLJ-073, from a dead 6-week-old male Pekingese with gross lesions and diarrhea. Interestingly, sequence analysis suggested that HLJ-073 contained a 350-nt deletion in ORF3abc compared with reference CCoV isolates, resulting in the loss of portions of ORF3a and ORF3c and the complete loss of ORF3b. Phylogenetic analysis based on the S gene showed that HLJ-073 was more closely related to members of the FCoV II cluster than to members of the CCoV I or CCoV II cluster. Furthermore, recombination analysis suggested that HLJ-073 originated from the recombination of FCoV 79-1683 and CCoV A76, which were both isolated in the United States. Cell tropism experiments suggested that HLJ-073 could effectively replicate in canine macrophages/ monocytes and human THP-1 cells. This is the first report of the isolation of strain HLJ-073 in China, and this virus has biological characteristics that are different from those of other reported CCoVs.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Molecular characterization and phylogenetic analysis of transmissible gastroenteritis virus HX strain isolated from China

Cited by 35 publications

References 17 publications

Isolation and characterization of a highly pathogenic strain ofPorcine enteric alphacoronaviruscausing watery diarrhoea and high mortality in newborn piglets

Isolation and characterization of a highly pathogenic strain ofPorcine enteric alphacoronaviruscausing watery diarrhoea and high mortality in newborn piglets

Occurrence and sequence analysis of porcine deltacoronaviruses in southern China

Molecular characterization of HLJ-073, a recombinant canine coronavirus strain from China with an ORF3abc deletion

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