Molecular characterization and phylogenetic analysis of Rhynchophorus ferrugineus (Olivier) in Eastern Province, Saudi Arabia

Abstract

Red palm weevil (RPW) is the most aggressive date palm parasite in the Middle East, and especially in the Gulf region. Originated in Southeast Asia, this pest has been detected in the entire Arabian Peninsula, North Africa, Italy, Latin America, and other territories. It is important to local from obtrusive species, which help augmenting the pest control strategies. In the present study we collected 21 RPW samples from 21 different locations in the Eastern Province, Saudi Arabia to genetically characterize them using RAPD- and ISSR-based clustering. Unweighted pair group method with arithmetic mean (UPGMA) for RAPD data categorized the 21 accessions into seven distinct groups, with Al-Oyonn and Juaymah each categorized in solitary group, meanwhile, UPGMA for ISSR indicated six different groups, with Battaliyah, Al-Oyoon, and Juaymah each assigned to a separate group. Combining RAPD and ISSR data revealed two accession; Al-Oyoon and Juaymah that might be considered obtrusive species. Based on distance calculations, we proposed that the potential origins of RPW collected from these locations are Iran and the United Arab Emirates. However, this assumption needs further studies for confirmation.

Omics in the Red Palm Weevil Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae): A Bridge to the Pest

“…All RNAi/CRISPR techniques could also benefit from improved reference genomes and some knowledge of the genetics of invasive RPW populations. To this day, population genetics of the RPW are restricted to the use of a single/few markers, mostly mitochondrial genes and microsatellite loci [ 98 , 99 , 100 , 101 ]. In this context, whole-genome sequencing of multiple field-collected individuals from diverse locations would be an important step toward understanding RPW population history and genetic diversity.…”

Identification of Morphology and Molecular PCR-RAPD Bactrocera spp. in the Location of Red Guava Crops, Deli Serdang District

“…According to Pramudi et al (2013) that the slightest change in the reaction can change the amount and intensity of the amplification product so repeatability is difficult to maintain. (Muladno 2021) also added that RAPD cannot distinguish homozygous and heterozygous individuals because it is a dominant marker and difficult to detect small changes in the structure of DNA (Boomibalagan et al 2021;Sabit et al 2021).…”

Effect of nutrient media enhanced with plant growth regulators on genetic stability in sub-cultures of Digitalis purpurea callus

“…The importance of studying genetic diversity among berry cultivars in selecting varieties with higher yields in fruits was previously reported [22]. It has been found that genetic variation was positive between genotypes and is useful for selecting appropriate groups for genomic mapping [14], and these genetic variations accumulated as a result of adaptation to different nutrient environments in artificial media, biotechnology methods that adopt bacteria to transfer the desirable traits in corps such as rice [38], thus stimulating physical, genetic variations as a result of adaptation, or non-adaptation to the components of the nutrient medium [10]. These findings support our results that showed different values of genetic stability in the studied media containing different types of plant growth regulators.…”

“…With the advancement of genetics and technologies used in molecular biology, many researches have focused on biotechnological methods adopted in the study of laboratory-bred plants and comparison of their production of effective biological materials in wild species and the resulting cell strains, and these technologies proved their effectiveness in determining genetic patterns [10,11]. The previous findings showed different concentrations of cardiac glycosides in wild, cultivated, and bred species of digitalis plants [12].…”