Molecular characterization and phylogenetic analysis of Escherichia coli isolated from milk of cattle affected by mastitis

Neamah, Azhar Abdulsada; Fahad, Khilood Hamdan; Sadeq, Jenan Nadhim; Alfatlawi, Monyer Abdulameir Abd

doi:10.33899/ijvs.2021.129934.1702

Cited by 6 publications

(6 citation statements)

References 20 publications

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“…The present study coliform pathogens high investigate &highly relationship to the score of CMT which is recorded ( 82.9% ) with later research reported high agreement between result of California test with bacteria detection [25]. Among different bacterial pathogens isolated, The current results showed that E. coli at (33.8%), Its agreement pattern was noticed in Iraq by reference [26], which is 36 % as predominant coliform pathogen than other species from milk contamination .further the our result high relatively show than by [27]which is 30% in Egypt. on the other hand, k.pneumonia constituted 11.7 % are the second prevalent bacteria finally Proteus mirabilis investigate as a low presented 10.2% from total isolate similar to [28,29].…”

Section: Identification and Isolationsupporting

confidence: 73%

Evaluation Effects of Coliform Mastitis on Hematological and Biochemical Parameters in Dairy Cows, in Kirkuk City, Iraq

Al-Bayati

2023

IOP Conf. Ser.: Earth Environ. Sci.

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This study conducted to investigate sub clinical mastitis caused by some coliform pathogens as a common causes mastitis in the cows and to detect some hematological & biochemical parameters, for this purpose 100 milk samples and100 blood samples were collected from apparently cows brought to the veterinary hospital & from different heifer’s in Kirkuk governate, Iraq. our results showed that the incidence of coliform mastitis by using different diagnostic methods. the incidence mastitis to CMT according of management & hygiene milk of processing recorded 35 (42.6 %), 47 ( 57.3%) respectively., However the bacteriologically examination depending on isolation, biochemical manually and vitek system identification. revealed, the incidence as 82.9% positive for bacterial identification reveled that E.coli was predominant ( 33.8 %) bacteria Followed by K. pneumonia and. Proteus mirabilis (11.7 %) &(10.2)% respectively. further hematological parameters we noted that showed anemia with significant decrease (p<0.05) in Hemoglobin, Total red blood cell count, Paced cell volume, lymphocyte and slightly decrease in, while record increase in granulocyte and Wight blood cell count. On the other hand the biochemical profile we showed significant increase in ALT, AST enzymes, While decrease significant(p<0.05) in ALP, Ca, Cl, P, Mg & zn. We conclude from this study, The prevalence of pathogenic bacteria in the milk resulted in a significant alteration in the hamato-biochemical profile in the current study reveled significant monitors for Bovine mastitis and estimate the efficiency and quality of milk consumption.

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Section: Identification and Isolationsupporting

confidence: 73%

Evaluation Effects of Coliform Mastitis on Hematological and Biochemical Parameters in Dairy Cows, in Kirkuk City, Iraq

Al-Bayati

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Using the Safe-Blue Illuminator/Electrophoresis System, electrophoresis was performed for 50 minutes at 120 volts. By comparing PCR result amplicons to a 100 bp DNA ladder, migration patterns were studied (29,30).…”

Section: Oligonucleotides and Pcr Amplificationmentioning

confidence: 99%

Detection of Campylobacter fetus in aborted ewes in Sulaimani province by PCR

Arif

2022

IJVS

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Abortion is one of the most critical factors affecting lambing rates and, as a result, sheep farm profitability. It is also significant from a zoonotic viewpoint, in addition to financial losses. In sheep flocks, Campylobacter fetus causes infectious infertility, embryonic death, and miscarriages. The study investigated C. fetus from aborted fetuses and vaginal swab samples collected from sheep flocks in the Sulaimani province by the polymerase chain reaction. Thirty-eight aborted fetuses and 70 vaginal swabs were collected from sheep flocks in three districts of Sulaimani province (Kalar, Said Sadiq, and Chamchamal) from March 2018 to June 2019. The pathogen was identified in clinical specimens using conventional PCR. C. fetus was isolated in 16 of 38 aborted fetuses 42.1% and 13 of 70 vaginal swabs from aborted ewes 18.6%. The C. fetus gene 16S rRNA was sequenced and received the accession number MW694741 in NCBI GenBank. Phylogenetic analysis of 16S rRNA gene sequences designated that the C. fetus isolates formed a separate branch displayed the highest similarity and clustered with MN203686.1 and EU773268.1 accessions in a specific clade. A lower degree of affinity of C. fetus was revealed with Campylobacter coli and Campylobacter jejuni.

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“…Electrophoresis conditions of include (75 V-300 mA -1h) using Wide Mini -Sub Cell GT (BioRad, USA). After that, the gel was observed using the (Gel-doc-Ez) system to revealing the specified bands (16,17).…”

Section: Detection Of Pseudomonas Aeruginosa and Their Virulence Gene...mentioning

confidence: 99%

Molecular identification of virulence genes of Pseudomonas aeruginosa isolated from fish (Cyprinus carpio) in Mosul city

Altaee¹,

Aldabbagh²

2022

IJVS

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Depending on the nature of the fish's environment, they are susceptible to many pathogens, including bacterial causes, so the goals of the current study are isolation and molecular identification of Pseudomonas aeruginosa with its prevalence rate and detected virulence genes from fresh common carp fish. The swabs were taken from the gills, skin, intestine and muscles of 75 fish samples from variable localities from Mosul city during the period September to December in the year 2021. The prevalence percentage of bacteria was 26.66% which was confirmed by traditional microbiological tests which included (phenotype culture, microscopically features and API-test) and molecular identification. The isolates formed 42.5, 37.5, 15, 5% from gills, skin, intestine and muscles, respectively. The molecular results of forty isolates determine that Pseudomonas aeruginosa have rpoB 100%, and virulence genes oprL, toxA, and algD, which are express the outer membrane protein, exotoxin A and alginate respectively occur as 97.5% for the oprL gene and 100% of both toxA and algD genes.

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Molecular characterization and phylogenetic analysis of Escherichia coli isolated from milk of cattle affected by mastitis

Cited by 6 publications

References 20 publications

Evaluation Effects of Coliform Mastitis on Hematological and Biochemical Parameters in Dairy Cows, in Kirkuk City, Iraq

Evaluation Effects of Coliform Mastitis on Hematological and Biochemical Parameters in Dairy Cows, in Kirkuk City, Iraq

Detection of Campylobacter fetus in aborted ewes in Sulaimani province by PCR

Molecular identification of virulence genes of Pseudomonas aeruginosa isolated from fish (Cyprinus carpio) in Mosul city

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