Molecular characterization and resistance profile of nosocomial Acinetobacter baumannii in intensive care unit of tertiary care hospital in Bangladesh

Khatun, Nazia; Farzana, Refath; Lopes, Bruno Silvester; Shamsuzzaman, SM

doi:10.3329/bmrcb.v41i2.29991

Cited by 11 publications

(9 citation statements)

References 26 publications

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“…27 A study conducted at the Dhaka Medical College Hospital (DMCH) showed 96% strains of A. baumannii isolated from endotracheal aspirates collected from patients, were multidrug resistant. 28 Another study carried in Square Hospitals Ltd. showed 90 % of the A. baumannii strains isolated from the patients with lower respiratory tract infections, were multidrug resistant. 29 In this study, 57% A. baumannii were MDR and 29% were XDR indicating an alarming situation.…”

Section: Discussionmentioning

confidence: 99%

Multidrug resistant clinical strains isolated from tracheal aspirates of patients in Dhaka, Bangladesh

et al. 2020

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Background: Antimicrobial resistance poses a major threat in the treatment of respiratory disease especially in developing countries like Bangladesh. Multidrug-resistant (MDR) bacteria along with extremely drug resistant (XDR) bacteria have emerged as major clinical and therapeutic dilemma in the treatment of tracheal infections here. Thus, the aim of this study is to assess multidrug resistance among clinical strains isolated from tracheal aspirates of patients in Dhaka, Bangladesh.Methods: Total 200 clinical isolates from tracheal aspirates were identified and their antibiotic susceptibility profiles were evaluated by using the VITEK 2 system following the Clinical and Laboratory Standards Institute guidelines. Patient information on diagnosis, sex, age was obtained from hospital data.Results: Of 200 clinical isolates obtained, Pseudomonas aeruginosa was the most frequent pathogens (30.5%) followed by Acinetobacter baumannii (29%), Klebsiella pneumoniae (22.5%), Streptococcus pneumoniae (7.5%), Escherichia coli (5%), Staphylococcus aureus (2%), Proteus spp (1.5%), Enterobacter spp (1%), Citrobacter spp (0.5%), Providencia spp (0.5%). Of 20 different antibiotics tested, highest number of isolates (86%) showed resistance to third generation cephalosporin cefixime, however least number of isolates showed resistance to polymixin antibiotics- colistin (12.5%) and polymixin B (6%). Tracheal infection was found to be more prevalent in males rather than in females although this difference was not statistically significant. The prevalence of infections was highest among the patients of age-group (old adults) ≥60 years (61.5%). Of 200 clinical isolates, 43 (21.5%) were XDR and 125 (62.5%) were MDR bacteria. Of 200 clinical isolates, the synthesis of extended spectrum β-lactamases (ESBL) and carbepenemase were detected in 59 (29.5%) and 98 (49%) strains respectively.Conclusions: Tracheal infections caused by β-lactamase producing MDR and XDR pathogens among patients are high in Dhaka, Bangladesh. Therefore, there is an urgent need for constant surveillance and interventions in Bangladesh in order to prevent further spreading of those resistant organisms.

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Section: Discussionmentioning

confidence: 99%

Multidrug resistant clinical strains isolated from tracheal aspirates of patients in Dhaka, Bangladesh

et al. 2020

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“…One more study examined endotracheal aspirates among patients of ICU reported that 96% of Acinetobacter baumannii were multidrug-resistant. The AMR (Antimicrobial resistance) genes such as bla ampC and carbapenemase genes ( bla MBLs and bla KPC ) were found in 95.83%, and 100% of A. baumanni were cephalosporin and imipenem resistant, respectively [63]. After that, the current study showed the MBL-producing gram-negative microorganism presence through multiplex PCR among VAP patients were most likely.…”

Section: Discussionmentioning

confidence: 62%

Comparative Study of CDST & Multiplex PCR to Detect MBL Producing Gram-Negative Bacilli among VAP Patients Admitted in a Public Medical College Hospital of Bangladesh

Nusrat¹,

Akter²,

Haque

et al. 2019

Pathogens

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: Background: Ventilator-associated pneumonia (VAP) is the most common nosocomial infection in intensive care units (ICU), which accounts for 25% of all ICU infection. Documenting carbapenem-resistant gram-negative bacilli is very important as these strains may often cause outbreaks in the ICU setting and are responsible for the increased mortality and morbidity or limiting therapeutic options. The classical phenotypic method cannot provide an efficient means of diagnosis of the metallo-β-lactamases (MBLs) producer. PCR (Polymerase chain reaction) assays have lessened the importance of the phenotypic approach by detecting metallo--lactamase resistance genes such as NDM （New Delhi metallo-β-lactamase）, IMP （Imipenemase）, VIM （Verona integron-encoded metallo-β-lactamase）, SPM （Sao Paulo metallo-β-lactamase）, GIM (Germany imipenemase）. Objective: To compare the results of the Combined Disc Synergy Test (CDST) with that of the multiplex PCR to detect MBL-producing gram-negative bacilli. Materials and Method: A total of 105 endotracheal aspirates (ETA) samples were collected from the ICU of a public school in Bangladesh. This cross-sectional study was carried out in the Department of Microbiology, Chittagong for quantitative culture, CDST test, and multiplex PCR for blaIMP, blaVIM, blaNDM genes of MBL producers. Results: Among the 105 clinically suspected VAP cases, the quantitative culture was positive in 95 (90%) and among 95 g-negative bacilli isolated from VAP patients, 46 (48.42%) were imipenem resistant, 30 (65.22%) were MBL producers by CDST, 21 (45.65%) were identified as MBL producers by multiplex PCR. Conclusion: PCR was highly sensitive and specific for the detection of MBL producers.

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“…baumannii is an important nosocomial pathogen in healthcare facilities and has become one of the most significant microorganisms causing infections in hospitalized patients in last few decades [28]. A study conducted at the Dhaka Medical College Hospital (DMCH) showed 96% strains of A. baumannii isolated from endotracheal aspirates collected from patients, were multidrug resistant [29]. Another study carried in Square Hospitals Ltd. showed 90 % of the A. baumannii strains isolated from the patients with lower respiratory tract infections, were multidrug resistant [30].…”

Section: Discussionmentioning

confidence: 99%

Community Acquired Multi-drug Resistant Clinical Strains from Tracheal Aspirates of Patients in Hospital Settings in Dhaka, Bangladesh

Sarkar¹,

Afroz²,

Jubyda³

et al. 2019

Preprint

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Background: Antimicrobial resistance is a multi-sectoral problem which poses a major threat in the treatment of infectious diseases especially in developing countries like Bangladesh. Multidrug-resistant (MDR) bacteria along with extremely drug resistant (XDR) bacteria have emerged as major clinical and therapeutic dilemma in the treatment of tracheal infections in hospitals here. Thus the aim of this study was to document the incidence of MDR and XDR producing β-lactamases in clinical isolates from tracheal aspirates of patients in Dhaka, Bangladesh. Methods: Two hundred clinical isolates from tracheal aspirates were identified and their antibiotic susceptibility profiles were evaluated by using the VITEK 2 system following the Clinical and Laboratory Standards Institute guidelines. Patient information on diagnosis, sex, age was obtained from hospital data. Results: Of 200 clinical, non-duplicate bacterial isolates obtained, Pseudomonas aeruginosa was the most frequent pathogens (N=61/200, 30.5%) followed by Acinetobacter baumannii (N=58/200, 29%), Klebsiella pneumoniae (N=45/200, 22.5%), Streptococcus pneumoniae (N = 15/200, 7.5%), Escherichia coli (N=10/200, 5%), Staphylococcus aureus (N=4/200, 2%), Proteus spp (N=3/200, 1.5%), Enterobacter spp (N=2/100, 1%), Citrobacter spp (1/200, 0.5%), Providencia spp (N=1/200, 0.5%). Of 20 different antibiotics tested, highest number of isolates (N=172/200, 86%) showed resistance to third generation cephalosporin cefixime, however least number of isolates showed resistance to polymixin antibiotics- colistin (N=25/200, 12.5%) and polymixinB (N=12/200, 6%) . The patients’ ages ranged between 1 month to 95 years with the gender distribution of 133 (66.5%) males and 67 (33.5%) females. The prevalence of infections was highest among the patients of age-group (old adults) ≥60 years (N=123/200, 61.5%). Of 200 clinical isolates, 43 (21.5%) were XDR and 125 (62.5%) were MDR bacteria. Of 200 clinical isolates, the synthesis of extended spectrum β-lactamases (ESBL) and carbepenemase were detected in 59 (29.5%) and 98 (49%) strains respectively. Conclusion: Tracheal infections caused by MDR and XDR pathogens among patients are high at hospital settings in Bangladesh. Therefore, there is an urgent need for constant surveillance and interventions in Bangladesh in order to prevent further spreading of those resistant organisms.

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Molecular characterization and resistance profile of nosocomial Acinetobacter baumannii in intensive care unit of tertiary care hospital in Bangladesh

Cited by 11 publications

References 26 publications

Multidrug resistant clinical strains isolated from tracheal aspirates of patients in Dhaka, Bangladesh

Multidrug resistant clinical strains isolated from tracheal aspirates of patients in Dhaka, Bangladesh

Comparative Study of CDST & Multiplex PCR to Detect MBL Producing Gram-Negative Bacilli among VAP Patients Admitted in a Public Medical College Hospital of Bangladesh

Community Acquired Multi-drug Resistant Clinical Strains from Tracheal Aspirates of Patients in Hospital Settings in Dhaka, Bangladesh

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