Molecular Characterization and their Phylogenetic Relationship Based on Mitochondrial Cytochrome Oxidase I of Brinjal Shoot and Fruit Borer, Leucinodes orbonalis (Guenée) (Lepidoptera: Pyralidae)

Murali, S.; Jalali, S. K.; Shylesha, A. N.; Swamy, T.M. Shivalinga; Baskar, Reeta; Mruthunjayaswamy, Prathibha

doi:10.20546/ijcmas.2017.607.298

Cited by 5 publications

(6 citation statements)

References 23 publications

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“…The values of pairwise F for all the populations were low ST to moderate, except in Bengaluru, which showed very high genetic differentiation from others (Table 4). The overall mean genetic distance was also found to be very low (0.004 ± 0.001), requirement based genetically distinct populations in Indian L. orbonalis as also suggested by Murali et al (2017) and Palraju et al (2018). All the populations also displayed a good number of mitochondrial haplotypes for the COI gene.…”

Section: Sequence Data Analysesmentioning

confidence: 69%

“…¨Journal of Environmental Biology, January 2022D etailed knowledge regarding genetic variations existing among insect pest populations along with understanding the mechanisms that drive genetic changes is prerequisite for devising long term pest management strategies (Murali et al, 2017). Study of pest population structure is also a more accurate means to determine phenotypic and genetic changes they undertake to tackle various survival hurdles (Sambathkumar et al, 2017).…”

Section: O N L I N E C O P Ymentioning

confidence: 99%

“…Thus, due to substantial gene flow, many of them often exhibit low genetic differentiation over their wide geographical distributional ranges (Sun et al, 2015). In case of L. orbonalis, both the active migration (because of good flight capacity) and passive migration (because of long distance transport of infested brinjal fruits) occurs, which might be helping it to maintain a steady state of gene flow (Murali et al, 2017), thereby minimizing genetic variation. The second reason can be that L. orbonalis has a narrow host range, and most of the commercially grown varieties of brinjal show little variation amongst themselves for their susceptibility against this pest species (Marimuthu et al, 2009).…”

Section: Sequence Data Analysesmentioning

confidence: 99%

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Genetic variability and population structure of Leucinodes orbonalis (Guenée), a severe insect pest of brinjal in India

Padwal¹,

Chakravarty²,

Srivastava³

2022

JEB

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Aim: The present study was undertaken to provide valuable insights regarding population genetic structure of Leucinodes orbonalis from diverse agro-ecologies of India. Methodology: Molecular characterization of L. orbonalis populations collected from five major agro-climatic zones of India was carried out using mitochondrial cytochrome oxidase I (COI) gene. Collected specimens were subjected to DNA extractions, partial PCR amplification and sequencing of the target gene, and multiple sequence alignments. Results: The results showed very less diversity in the nucleotide positions of the COI sequences of 79 studied specimens, with a low number of segregating sites (30), nucleotide diversity (0.00438) and overall mean genetic distance (0.004 ± 0.001). The significant negative values of neutrality tests and unimodal mismatch distribution supported the demographic expansion theory in Indian L. orbonalis. Analysis of the molecular variance revealed that 93.13% of the genetic variation was within populations, and the variation among populations was only 6.87%. The pairwise genetic differentiation was also found to be low to moderate between most of the populations. Multiple haplotypes were recorded from all the populations, and both neighbour-joining tree as well as the haplotype network showed that clustering of the haplotypes was independent of the geographical location. Interpretation: Thus, it can be inferred that Indian populations of L. orbonalis have very low genetic variation levels concerning the COI gene. There is a possible occurrence of stable inherited gene flow among populations, thereby reducing genetic variation in India.

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Section: Sequence Data Analysesmentioning

confidence: 69%

Section: O N L I N E C O P Ymentioning

confidence: 99%

Section: Sequence Data Analysesmentioning

confidence: 99%

See 1 more Smart Citation

Genetic variability and population structure of Leucinodes orbonalis (Guenée), a severe insect pest of brinjal in India

Padwal¹,

Chakravarty²,

Srivastava³

2022

JEB

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“…Systematic research such as collection, conservation, characterization and evaluation, and utilization of brinjal germplasm has been done at PGRC of BARI. Few studies on both morpho-genetic and molecular level have been done in different countries but few or none reports included accessions from Bangladesh (Murali et al, 2017;Sunseri et al, 2010;Demir et al, 2010;Islam andUddin 2009, Khorsheduzzaman et al, 2008;Kumer et al, 2008). Characterization and evaluation of plant germplasm is imperative for categorization of germplasm and identification of desirable genotypes carried out in precision fields under sufficient growth and plant protection conditions for utilization in breeding programs (Upadhyaya et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Characterization and genetic diversity of brinjal germplasm

Islam

Chhanda

Pervin

et al. 2018

Bangladesh J. Agric. Res

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The experiment was carried out in a non replicated design with 40 accessions of brinjal (Solanum melongena L.) at Plant Genetic Resources Centre (PGRC), BARI Gazipur to characterize and study the genetic diversity and identify the useful traits. Each accession of nine plants was grownin a 3 X 2 m size plot. The accessions were collected from 16 districts of Bangladesh. Two to four classes were found for plant growth habit, leaf blade lobing, leaf prickles, flower colour, fruit shape, fruit apex shape and fruit colour distribution. The accessions exhibited 64% straight, 13% both slightly curved and curved, and 10% snaked shaped edible fruit along with 55% purple and 45% green fruit. Fruit length of accessions ranged from 5 to 31.23 cm, breadth 2.4 to 10.6 cm, weight 13 to 95.2 g and 3 to 30 fruits per plant. The accessions were grouped into five clusters. The inter and intra cluster distances ranged from 4.01 to 8.32 and 0.94 to 1.36, respectively. Accessions collected from the same districts felt into different clusters. The results obtained by D2 analysis were also confirmed by canonical analysis. Crosses BD-7327 (Rangpur) of cluster II with BD-9954 (Chittagong) of Cluster-III and BD-7319 (Panchagarh) of Cluster-I with BD-11732 (Bandarban) of cluster V, could be performed for obtaining wider variability in the segregating generations. The breeders could use the selected accessions for varietal improvement of brinjal.Bangladesh J. Agril. Res. 43(3): 499-512, September 2018

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“…A part of the mitochondrial cytochrome oxidase I (COI) gene was proposed to serve as ‘DNA Barcode’ to identify animal species based on their unique group of DNA Barcode sequences (Hebert et al., 2003 ). Therefore, ‘DNA Barcode’ sequences were compiled from several publications on the Austral‐Asian L. orbonalis (Chang et al., 2014 ; Mally et al., 2015 ; Sagarbarria et al., 2018 ; Shashank et al., 2015 ; Murali et al., 2017 ; Palraju et al., 2018 ; Karthika et al., 2019 ; Natikar et al., 2022 ; Padwal et al., 2022 ), DNA Barcode sequences available in the Barcode of Life Database and sequences not published in the literature but submitted as ‘unpublished’ to GenBank. We assembled a total of 398 sequences into a DNA alignment (Data S1 ) and used the R packages APE (Paradis et al., 2004 ) and SPIDER (Brown et al., 2012 ) as well as the FigTree v1.4.4 software (Rambaut, 2006–2018 ) to analyse the data and illustrate a neighbour‐joining tree (Figure A.1 ).…”

Section: Figure A1mentioning

confidence: 99%

Pest risk assessment of Leucinodes orbonalis for the European Union

Bragard,

Baptista,

Chatzivassiliou

et al. 2024

EFS2

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Following a request from the European Commission, the EFSA Panel on Plant Health performed a quantitative risk assessment of Leucinodes orbonalis (Lepidoptera: Crambidae), the eggplant fruit and shoot borer, for the EU. The assessment focused on potential pathways for entry, climatic conditions favouring establishment, spread and impact. Options for risk reduction are discussed but effectiveness was not quantified. L. orbonalis is a key pest of eggplant (aubergine/brinjal) in the Indian subcontinent and occurs throughout most of southern Asia with records mostly from India and Bangladesh. The main pathway of entry is fruit of solanaceous plants, primarily exotic varieties of eggplant, Solanum melongena and turkey berry, S. torvum. The trade in both commodities from Asia is small but nevertheless dwarfs the trade in other Solanum fruits from Asia (S. aethiopicum, S. anguivi, S. virginianum, S. aculeatissimum, S. undatum). Other Solanum fruits were therefore not further assessed as potential pathways. The trade in eggplant from Asia consists of special fruit types and caters mostly to niche markets in the EU, while most eggplant consumed in Europe is produced in southern European and northern African countries, where L. orbonalis does not occur. Using expert knowledge elicitation (EKE) and pathway modelling, the Panel estimated that approximately 3-670 infested fruit (90% certainty range, CR) of S. melongena or fruit bunches of S. torvum enter into regions of the EU that are suitable for L. orbonalis establishment each year. Based on CLIMEX modelling, and using two possible thresholds of ecoclimatic index (EI) to indicate uncertainty in establishment potential, climates favouring establishment occur mostly in southern Europe, where, based on human population, approximately 14% of the imported produce is distributed across NUTS2 regions where EI ≥ 30; or 23% of the produce is distributed where EI ≥ 15. Escape of adult moths occurs mostly from consumer waste. By analysing results of different scenarios for the proportion of S. melongena and S. torvum in the trade, and considering uncertainties in the climatic suitability of southern Europe, adult moth emergence in areas suitable for establishment is expected to vary between 84 individuals per year and one individual per 40 years (based on 90% CR in different scenarios). In the baseline scenario, 25% of the solanaceous fruit from Asia

show abstract

Molecular Characterization and their Phylogenetic Relationship Based on Mitochondrial Cytochrome Oxidase I of Brinjal Shoot and Fruit Borer, Leucinodes orbonalis (Guenée) (Lepidoptera: Pyralidae)

Cited by 5 publications

References 23 publications

Genetic variability and population structure of Leucinodes orbonalis (Guenée), a severe insect pest of brinjal in India

Genetic variability and population structure of Leucinodes orbonalis (Guenée), a severe insect pest of brinjal in India

Characterization and genetic diversity of brinjal germplasm

Pest risk assessment of Leucinodes orbonalis for the European Union

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