Molecular characterization and tissue distribution of carnitine palmitoyltransferases in Chinese mitten crab Eriocheir sinensis and the effect of dietary fish oil replacement on their expression in the hepatopancreas

Abstract

The carnitine palmitoyltransferase (CPT) family includes CPT 1 and CPT 2 that transport long-chain fatty acids into the mitochondrial compartment for β-oxidation. In this study, three isoforms (CPT 1α, CPT 1β and CPT 2) of the CPT family were cloned from Chinese mitten crab (Eriocheir sinensis) and their complete coding sequences (CDS) were obtained. Sequence analysis revealed deduced amino acid sequences of 915, 775 and 683 amino acids, respectively. Gene expression analysis revealed a broad tissue distribution for all three isoforms, with high CPT 1α and CPT 2 mRNA levels in the hepatopancreas of males and females. In males, CPT 1β was highly expressed in gill, heart, brain ganglia and muscle, while in females, CPT 1β-mRNA levels were relatively high in muscle, hepatopancreas and ovary tissue. The effects of dietary fish oil replacement on the expression of the three CPT isoforms in the hepatopancreas during gonadal development were investigated using five experimental diets formulated with replacement of 0, 25, 50, 75 and 100% fish oil by 1:1 rapeseed oil: soybean oil. The results showed that Diets 2# and 5# yielded higher CPT 1α and CPT 2 mRNA expression in males (P < 0.05), while in females, expression of all three CPT isoforms increased then declined in the hepatopancreas with increasing dietary fish oil replacement. The observed changes in CPT gene expression varied in different isoforms and gender, suggesting the three CPT genes might play different roles in fatty acid β-oxidation in E. sinensis.

Effects of fish oil replacement by blending vegetable oils in fattening diets on nonvolatile taste substances of swimming crab ( Portunus trituberculatus )

“…About 75% vegetable oils replacing fish oil can appropriately increase the spawning and fertility of river crabs (Liu, Jiang, Wu, Deng, & Cheng, 2017). Fish oil replacement by vegetable oils (canola oil: soybean oil = 1:1) affects the mRNA levels in the hepatopancreas for carnitine palmitoyl transferase isoforms which may associate with fatty acid β‐oxidation in Chinese mitten crab (Liu, Long, Deng, Cheng, & Wu, 2018).…”

Multi-omics analysis to examine microbiota, host gene expression and metabolites in the intestine of black tiger shrimp (Penaeus monodon) with different growth performance

“…Acylcarnitines play vital role in balancing sugar and lipid catabolism. They act as carriers for transportation of long-chain fatty acids to catabolize through fatty acid β-oxidation, leading to energy production in Zebrafish (Li et al, 2017) and in Chinese mitten crab (Liu et al, 2018). Malic acid is an intermediate metabolite in TCA cycle involved in energy metabolism playing a role on growth performance in crustaceans (Hervant, 1996), fish and crayfish (Skorkowski, 1988).…”

Molecular characteristic of activin receptor IIB and its functions in growth and nutrient regulation in Eriocheir sinensis

“…Similarly, at the end of the RNAi experiment, six crabs from the RNAi and control groups were sampled, and their hepatopancreas and leg muscle tissues were collected for qRT-PCR detection of the target genes, including five ActRIIB signaling pathway genes ( ActRI , SMAD3 , SMAD4 , forkhead box O ( FoxO ), and mammalian target of rapamycin ( mTOR )) ( Han et al, 2013 ; Guru et al, 2019 ), and three lipid metabolism-related genes (carnitine palmitoyltransferases 1β ( CPT1 β ), fatty acid synthase ( FAS ), and fatty acid elongation ( FAE )) ( Liu et al, 2016 ; DeBose-Boyd, 2018 ; Liu et al, 2018 ). The qRT-PCR procedures were similar to those for Es-ActRIIB expression detection.…”