Molecular characterization and tissue distribution of ferritin M in kelp grouper, Epinephelus bruneus

Harikrishnan, Ramasamy; Kim, Ju-Sang; Jang, Ik-Soo; Kim, Man-Chul; Balasundaram, Chellam; Heo, Moon‐Soo

doi:10.1016/j.jbiosc.2011.07.023

Cited by 4 publications

(5 citation statements)

References 19 publications

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“…In this study, it was observed that the increase in MCV especially in pesticide‐exposed and self‐healing groups was a macrocytic result due to swelling of erythrocytes and pointed to anemia. This situation increases the affinity to the blood oxygen …”

Section: Discussionmentioning

confidence: 99%

The effect of N‐acetylcysteine supplementation on the oxidative stress levels, apoptosis, DNA damage, and hematopoietic effect in pesticide‐exposed fish blood

Uçar

Özgeriş

Yeltekin

et al. 2019

J Biochem & Molecular Tox

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Cysteine is important for protein synthesis, detoxification, and diverse metabolic functions.However, cysteine metabolism has been poorly described in fish, and the role of the therapeutic effect in pesticide toxicology on aquatic organisms is unknown. The aim of this study was to determine the effects of regular cysteine treatment on the hematology, biochemistry, apoptosis, oxidative DNA damage, and antioxidant parameters in fish blood after chemical application. Therefore, fish were exposed to cypermethrin for 2 weeks. Then two different concentrations of N-acetylcysteine (NAC) were applied for a 4-day treatment period and compared with the group of the self-healing process. At the end of the treatment, the hematological index, blood biochemical parameters, paraoxonase (PON), arylesterase (ARE), and myeloperoxidase (MPO) activities in the fish blood samples were investigated. With regard to the hematological parameters, statistical differences were obtained except for mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) (P < 0.05). Enzyme activities (ARE, PON, and MPO), as well as some biochemical parameters (creatinin [Cre], alanine amino transferase, total glyceride, alkaline phosphatase, iron, calcium, low density lipoprotein-cholesterol [LDL-C],sodium, and potassium), were found to be importantly different among all groups at the P < 0.05 level, while 8-hydroxydeoxyguanosine and caspase-3 levels were determined to be high in the pesticide group but decreased significantly in NAC-treated groups (P < 0.05).According to the results of the study, acute cysteine treatment showed an ameliorative effect on the hematological index, biochemical parameters, PON, MPO, and ARE in the blood in the all the treatment group fish. The positive effect of NAC on protein synthesis, detoxification, and diverse metabolic functions against cypermethrin toxicity was more effective in 1.0 mM NAC. NAC has an important therapeutic effect on pesticide-induced hematoxicity for fish in terms of all the data. K E Y W O R D S caspase-3, fish, hematology, MPO, NAC, 8-OHdG

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Section: Discussionmentioning

confidence: 99%

The effect of N‐acetylcysteine supplementation on the oxidative stress levels, apoptosis, DNA damage, and hematopoietic effect in pesticide‐exposed fish blood

Uçar

Özgeriş

Yeltekin

et al. 2019

J Biochem & Molecular Tox

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“…Ferritin synthesis is known to be regulated by pathogen infection (Andersen et al 1995(Andersen et al , 1998Mignogna et al 2002;Zhang et al 2010;Zheng et al 2010;Harikrishnan et al 2011;Wang et al 2011;Elvitigala et al 2013;Lee et al 2014). For example, upon exposure to bacterial pathogens, the expressions of the M ferritins of rock bream, tongue sole, turbot, and large yellow croaker are enhanced (Zhang et al 2010;Zheng et al 2010;Wang et al 2011;Elvitigala et al 2013), whereas the expression of the M ferritin of Atlantic salmon is inhibited .…”

Section: Discussionmentioning

confidence: 99%

“…In fish, M ferritin has been identified in a number of species including Oplegnathus fasciatus, Chionodraco rastrospinosus, Epinephelus bruneus, Cynoglossus semilaevis, Scophthalmus maximus, Pseudosciaena crocea, Trematomus bernacchii, and Salmo salar (Andersen et al 1995(Andersen et al , 1998Mignogna et al 2002;Giorgi et al 2008;Zhang et al 2010;Harikrishnan et al 2011;Wang et al 2011;Elvitigala et al 2013;Scudiero et al 2013;Lee et al 2014). However, only a few of these ferritins were shown to possess iron binding and/or antioxidative potential.…”

Section: Introductionmentioning

confidence: 99%

Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties

Wang

Sun

2015

Fish Physiol Biochem

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Ferritin is an evolutionarily conserved protein that plays a vital role in maintaining iron homeostasis. In this study, we identified a ferritin M (PoFerM) from Japanese flounder (Paralichthys olivaceus) and analyzed its biological property. PoFerM is composed of 176 amino acid residues and contains the conserved ferroxidase diiron center and the ferrihydrite nucleation center typical of M ferritins. Expression of PoFerM occurred in multiple tissues and was most abundant in blood. Bacterial infection upregulated PoFerM expression in head kidney, spleen, and liver in a time-dependent manner. Recombinant PoFerM (rPoFerM) purified from Escherichia coli exhibited iron-chelating activity and inhibited bacterial growth, whereas rPoFerMM, the mutant protein that bears alanine substitution at two conserved residues of the ferroxidase center and the ferrihydrite nucleation center, failed to do so. Oxidative protection analysis showed that rPoFerM, but not rPoFerMM, was able to alleviate the deleterious effect of H2O2-induced free radicals on plasmid DNA and primary flounder cells. Together these results indicate that PoFerM is an iron chelator with antimicrobial and antioxidative properties, all which depend on the conserved ferroxidase center and the ferrihydrite nucleation site.

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“…In order to confirm that the DNAs were not contaminated, no-cDNA controls (NC) and no-reverse-transcriptase controls (RT−) for each sample were thermocycled under the same conditions. Primers for the β-actin gene [44] were used as a positive control (Appendix 1 in the ESM). The conditions for this experiment followed the RT-PCR method described in the "RT-PCR" section above.…”

Section: Opsin Gene Expressionmentioning

confidence: 99%

Sequence analysis and expression patterns of opsin genes in the longtooth grouper Epinephelus bruneus

Matsumoto

Ishibashi

2015

Fish Sci

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Molecular characterization and tissue distribution of ferritin M in kelp grouper, Epinephelus bruneus

Cited by 4 publications

References 19 publications

The effect of N‐acetylcysteine supplementation on the oxidative stress levels, apoptosis, DNA damage, and hematopoietic effect in pesticide‐exposed fish blood

The effect of N‐acetylcysteine supplementation on the oxidative stress levels, apoptosis, DNA damage, and hematopoietic effect in pesticide‐exposed fish blood

Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties

Sequence analysis and expression patterns of opsin genes in the longtooth grouper Epinephelus bruneus

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