Molecular characterization, genomic structure and expressional profiles of a CXC chemokine receptor 4 (CXCR4) from rock bream Oplegnathus fasciatus

Thulasitha, William Shanthakumar; Umasuthan, Navaneethaiyer; Revathy, Kasthuri Saranya; Whang, Ilson; Lee, Jehee

doi:10.1016/j.fsi.2015.03.012

Cited by 14 publications

(13 citation statements)

References 50 publications

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“…Similarly, GPCRs can respond to diverse sensory ligands, including many odorants, light sensitive compounds, pheromones, and neurotransmitters (Brody and Cravchik 2000;Hewes and Taghert 2001). In line with other researchers, we assume that the levels of GPCR mRNAs give an indication of the activation of GPCR proteins (e.g., Churcher et al 2015;Thulasitha et al 2015;Quandt et al 2016), though it is also possible that GPCR genes could be transcriptionally activated by a pathway not involving the same GPCR. There was a strong and consistent profile of early up-regulation followed by later down-regulation and then dampening of expression, again consistent with the activation of alternative sensory modalities.…”

Section: Thorax (Ht) and An Inconsistent Up-regulation Of Ejaculate Csupporting

confidence: 53%

Genomic responses to the socio-sexual environment in male Drosophila melanogaster exposed to conspecific rivals

Mohorianu

Bretman

Smith

et al. 2017

RNA

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Socio-sexual environments have profound effects on fitness. Local sex ratios can alter the threat of sexual competition, to which males respond via plasticity in reproductive behaviors and ejaculate composition. In Drosophila melanogaster, males detect the presence of conspecific, same-sex mating rivals prior to mating using multiple, redundant sensory cues. Males that respond to rivals gain significant fitness benefits by altering mating duration and ejaculate composition. Here we investigated the underlying genome-wide changes involved. We used RNA-seq to analyze male transcriptomic responses 2, 26, and 50 h after exposure to rivals, a time period that was previously identified as encompassing the major facets of male responses to rivals. The results showed a strong early activation of multiple sensory genes in the head-thorax (HT), prior to the expression of any phenotypic differences. This gene expression response was reduced by 26 h, at the time of maximum phenotypic change, and shut off by 50 h. In the abdomen (A), fewer genes changed in expression and gene expression responses appeared to increase over time. The results also suggested that different sets of functionally equivalent genes might be activated in different replicates. This could represent a mechanism by which robustness is conferred upon highly plastic traits. Overall, our study reveals that mRNA-seq can identify subtle genomic signatures characteristic of flexible behavioral phenotypes.

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Section: Thorax (Ht) and An Inconsistent Up-regulation Of Ejaculate Csupporting

confidence: 53%

Genomic responses to the socio-sexual environment in male Drosophila melanogaster exposed to conspecific rivals

Mohorianu

Bretman

Smith

et al. 2017

RNA

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“…Cxcr4a and cxcr4b have been identified in zebrafish ( Danio rerio ) [ 25 , 26 ], Asian swamp eel ( Monopterus albus ) [ 27 ], and channel catfish ( Ictalurus punctatus ) [ 28 ]. In addition, one paralog of cxcr4 has been characterized in several telesots [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]: medaka ( Oryzias latipes ) [ 29 ], turbot ( Scophthalmus maximus ) [ 30 ], orange-spotted grouper ( Epinephelus coioides ) [ 31 ], and so on. The expression and function of cxcl12s - cxcr4s have been well documented in zebrafish [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…The expression and function of cxcl12s - cxcr4s have been well documented in zebrafish [ 4 ]. Additional studies have focused on the dynamic expression changes in response to pathogens or hypoxia stress [ 28 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. However, detailed research on comparative synteny and evolution analysis, and divergent expression patterns and function of the two cxcr4 paralogs in teleost is scarce.…”

Section: Introductionmentioning

confidence: 99%

Divergent Expression Patterns and Function of Two cxcr4 Paralogs in Hermaphroditic Epinephelus coioides

Zhou

Gao

et al. 2018

IJMS

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Chemokine receptor Cxcr4 evolved two paralogs in the teleost lineage. However, cxcr4a and cxcr4b have been characterized only in a few species. In this study, we identified two cxcr4 paralogs from the orange-spotted grouper, Epinephelus coioides. The phylogenetic relationship and gene structure and synteny suggest that the duplicated cxcr4a/b should result from the teleost-specific genome duplication (Ts3R). The teleost cxcr4 gene clusters in two paralogous chromosomes exhibit a complementary gene loss/retention pattern. Ec_cxcr4a and Ec_cxcr4b show differential and biased expression patterns in grouper adult tissue, gonads, and embryos at different stages. During embryogenesis, Ec_cxcr4a/b are abundantly transcribed from the neurula stage and mainly expressed in the neural plate and sensory organs, indicating their roles in neurogenesis. Ec_Cxcr4a and Ec_Cxcr4b possess different chemotactic migratory abilities from the human SDF-1α, Ec_Cxcl12a, and Ec_Cxcl12b. Moreover, we uncovered the N-terminus and TM5 domain as the key elements for specific ligand–receptor recognition of Ec_Cxcr4a-Ec_Cxcl12b and Ec_Cxcr4b-Ec_Cxcl12a. Based on the biased and divergent expression patterns of Eccxcr4a/b, and specific ligand–receptor recognition of Ec_Cxcl12a/b–Ec_Cxcr4b/a, the current study provides a paradigm of sub-functionalization of two teleost paralogs after Ts3R.

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“…The CXCR4 expression level of kidney, gill, spleen and blood was higher when compared with other fish species such as rock bream, turbot, miiuy croaker and rainbow trout. 51,52,55 Liver, kidney had shown a higher expression of CXCR4 in Megalobrama amblycephala. 50 From previous studies, it has been established that CXCR4 can be controlled with the help of negative regulation of immune factors by avoiding the excess inflammation.…”

Section: Discussionmentioning

confidence: 96%

“…In fish, CXCR3 molecules have been characterized in Megalobrama amblycephala, Scophthalmus maximus, Oplegnathus fasciatus, Epinephelus coioides, Monopterus albus, miiuy croaker, Ctenopharyngodon idella and medaka Oryzias latipes. [50][51][52][53][54][55][56][57] CXCR3 was first reported in myeloid cell development of zebra fish 58 and in medaka it was found to respond in wound healing. 22 CXCR4 gene was also characterized in rainbow trout, starlet, zebra fish and turbot 22,59,60 which proved to show its expression in regulation of immune system due to cytokine growth factors and intracellular second messengers.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, characterization and expression analysis of MHCI and chemokines CXCR3 and CXCR4 gene from freshwater carp,Catla catla

Banerjee

Roy

Samanta

et al. 2019

Microbiology and Immunology

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The immune system with large number of molecules protects the host against a plethora of continuously evolving microbes. Major histocompatibility complex (MHC) molecules serve as cardinal elements of the adaptive immune system responsible for the activation of the adaptive immunity in the host. The present study reports MHCI molecule in freshwater carp, Catla catla, and its differential expression in immunologically relevant tissues post-infection with Gramnegative and Gram-positive bacteria. The MHCI sequence of C. catla had 502 bp nucleotides encoding putative 146 amino acids. The phylogenetic analysis exhibited its evolutionary conservation within the Cyprinidae family and formed a different clade with the higher vertebrates. Simultaneously, CXCR3 and CXCR4 chemokines were cloned and characterized for their expression in infected tissues. Analysis of immunologically relevant tissues of the infected fish exhibited an increase of MHCI gene expression and the down-regulation of CXCR3 and CXCR4 chemokines, indicating a tricky interaction between the innate and adaptive immune system. It was found that intestine, skin and spleen played a crucial role in the contribution of the defense activity which instigated the self-immunity. These immune activities can provide useful information to understand the interaction of self and non-self-immune system in freshwater fish, Catla catla.

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Molecular characterization, genomic structure and expressional profiles of a CXC chemokine receptor 4 (CXCR4) from rock bream Oplegnathus fasciatus

Cited by 14 publications

References 50 publications

Genomic responses to the socio-sexual environment in male Drosophila melanogaster exposed to conspecific rivals

Genomic responses to the socio-sexual environment in male Drosophila melanogaster exposed to conspecific rivals

Divergent Expression Patterns and Function of Two cxcr4 Paralogs in Hermaphroditic Epinephelus coioides

Molecular cloning, characterization and expression analysis of MHCI and chemokines CXCR3 and CXCR4 gene from freshwater carp,Catla catla

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